GUTI: a new antigen in the Cromer blood group system

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dc.contributor.authorStorry, JR
dc.contributor.authorSausais, L
dc.contributor.authorHue Roye, K
dc.contributor.authorMudiwa, F
dc.contributor.authorFerrer, Z
dc.contributor.authorBlajchman, MA
dc.contributor.authorLublin, DM
dc.contributor.authorMa, BW
dc.contributor.authorMiquel, JE
dc.contributor.authorNervi, F
dc.contributor.authorPereira, J
dc.contributor.authorReid, ME
dc.date.accessioned2024-01-10T13:48:56Z
dc.date.available2024-01-10T13:48:56Z
dc.date.issued2003
dc.description.abstractBACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI.
dc.description.abstractSTUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using Maell was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI.
dc.description.abstractRESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4.
dc.description.abstractCONCLUSION: This study describes a novel high-incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.
dc.description.funderNHLBI NIH HHS
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.fechaingreso.objetodigital2024-05-02
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1046/j.1537-2995.2003.00319.x
dc.identifier.issn0041-1132
dc.identifier.pubmedidMEDLINE:12675719
dc.identifier.urihttps://doi.org/10.1046/j.1537-2995.2003.00319.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79409
dc.identifier.wosidWOS:000181732600009
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.information.autorucMedicina;Nervi F;S/I;99156
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final344
dc.pagina.inicio340
dc.publisherWILEY-BLACKWELL
dc.revistaTRANSFUSION
dc.rightsacceso restringido
dc.subjectDECAY-ACCELERATING FACTOR
dc.subjectMOLECULAR-BASIS
dc.subjectPHENOTYPE
dc.subjectDEFICIENCY
dc.subjectEPITOPES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGUTI: a new antigen in the Cromer blood group system
dc.typeartículo
dc.volumen43
sipa.codpersvinculados72216
sipa.codpersvinculados99156
sipa.codpersvinculados99371
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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