Localization of the Sodium-Taurocholate cotransporting polypeptide in membrane rafts and modulation of its activity by cholesterol in vitro

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dc.contributor.authorMolina, Hector
dc.contributor.authorAzocar, Lorena
dc.contributor.authorAnanthanarayanan, Meenakshisundaram
dc.contributor.authorArrese, Marco
dc.contributor.authorMiquel, Juan Francisco
dc.date.accessioned2024-01-10T12:40:06Z
dc.date.available2024-01-10T12:40:06Z
dc.date.issued2008
dc.description.abstractBackground: The relevance of discrete localization of hepatobiliary transporters in specific membrane microdomains is not well known. Aim: To determine whether the Na+/taurocholate cotransporting polypeptide (Ntcp), the main hepatic sinusoidal bile salt transporter, is localized in specific membrane microdomains. Methods: Presence of Ntcp in membrane rafts obtained from mouse liver was studied by immunoblotting and immunofluorescence. HEK-293 cells stably transfected with rat Ntcp were used for in vitro studies. Expression, localization and function of Ntcp in these cells were assessed by immunoblotting, immunofluorescence and biotinylation studies and Na+-dependent taurocholate uptake assays, respectively. The effect of cholesterol depletion/repletion assays on Ntcp function was also investigated. Results: Ntcp localized primarily to membrane rafts in in vivo studies and localized partially in membrane rafts in transfected HEK-293 cells. In these cells, membrane cholesterol depletion resulted in a shift of Ntcp localization into non-membrane rafts, which correlated with a 2.5-fold increase in taurocholate transport. Cholesterol repletion shifted back part of Ntcp into membrane rafts, and normalized taurocholate transport to values similar to control cells. Conclusion: Ntcp localizes in membrane rafts and its localization and function are regulated by membrane cholesterol content. This may serve as a novel regulatory mechanism of bile salt transport in liver. (C) 2008 Elsevier B.V. All rights reserved.
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
dc.description.funderNICHD NIH HHS
dc.fechaingreso.objetodigital19-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.bbamem.2008.01.020
dc.identifier.issn0005-2736
dc.identifier.pubmedidMEDLINE:18328802
dc.identifier.urihttps://doi.org/10.1016/j.bbamem.2008.01.020
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77272
dc.identifier.wosidWOS:000256211700010
dc.information.autorucMedicina;Arrese M;S/I;76095
dc.information.autorucMedicina;Azócar L;S/I;1006324
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.information.autorucMedicina;Molina H;S/I;1001203
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1291
dc.pagina.inicio1283
dc.publisherELSEVIER SCIENCE BV
dc.revistaBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
dc.rightsacceso restringido
dc.subjectbile acid
dc.subjectcaveolin
dc.subjectlipid microdomain
dc.subjectlipid raft
dc.subjectmembrane transport
dc.subjectP-GLYCOPROTEIN ACTIVITY
dc.subjectLIPID RAFTS
dc.subjectPLASMA-MEMBRANE
dc.subjectRAT-LIVER
dc.subjectTRANSPORT-SYSTEMS
dc.subjectEPITHELIAL-CELLS
dc.subjectBILE SECRETION
dc.subjectMICRODOMAINS
dc.subjectTRAFFICKING
dc.subjectNTCP
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLocalization of the Sodium-Taurocholate cotransporting polypeptide in membrane rafts and modulation of its activity by cholesterol in vitro
dc.typeartículo
dc.volumen1778
sipa.codpersvinculados76095
sipa.codpersvinculados1006324
sipa.codpersvinculados72216
sipa.codpersvinculados1001203
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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