Clot lysis time in platelet-rich plasma: Method assessment, comparison with assays in platelet-free and platelet-poor plasmas, and response to tranexamic acid

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dc.contributor.authorPanes, Olga
dc.contributor.authorPadilla, Oslando
dc.contributor.authorMatus, Valeria
dc.contributor.authorSaez, Claudia G.
dc.contributor.authorBerkovits, Alejandro
dc.contributor.authorPereira, Jaime
dc.contributor.authorMezzano, Diego
dc.date.accessioned2024-01-10T12:38:05Z
dc.date.available2024-01-10T12:38:05Z
dc.date.issued2012
dc.description.abstractFibrinolysis dysfunctions cause bleeding or predisposition to thrombosis. Platelets contain several factors of the fibrinolytic system, which could up or down regulate this process. However, the temporal relationship and relative contributions of plasma and platelet components in clot lysis are mostly unknown. We developed a clot lysis time (CLT) assay in platelet-rich plasma (PRP-CLT, with and without stimulation) and compared it to a similar one in platelet-free plasma (PFP) and to another previously reported test in platelet-poor plasma (PPP). We also studied the differential effects of a single dose of tranexamic acid (TXA) on these tests in healthy subjects. PFP- and PPP-CLT were significantly shorter than PRP-CLT, and the three assays were highly correlated (p < 0.0001). PFP- and PPP-, but more significantly PRP-CLT, were positively correlated with age and plasma PAI-1, von Willebrand factor, fibrinogen, LDL-cholesterol, and triglycerides (p < 0.001). All these CLT assays had no significant correlations with platelet aggregation/secretion, platelet counts, and pro-coagulant tests to explore factor X activation by platelets, PRP clotting time, and thrombin generation in PRP. Among all the studied variables, PFP-CLT was independently associated with plasma PAI-1, LDL-cholesterol, and triglycerides and, additionally, stimulated PRP-CLT was also independently associated with plasma fibrinogen. A single 1 g dose of TXA strikingly prolonged all three CLTs, but in contrast to the results without the drug, the lysis times were substantially shorter in non-stimulated or stimulated PRP than in PFP and PPP. This standardized PRP-CLT may become a useful tool to study the role of platelets in clot resistance and lysis. Our results suggest that initially, the platelets enmeshed in the clot slow down the fibrinolysis process. However, the increased clot resistance to lysis induced by TXA is overcome earlier in platelet-rich clots than in PFP or PPP clots. This is likely explained by the display of platelet pro-fibrinolytic effects. Focused research is needed to disclose the mechanisms for the relationship between CLT and plasma cholesterol and its potential pathophysiologic and clinical relevance.
dc.description.funderFONDECYT (Fondo de Desarrollo Cientifico y Tecnologico de Chile)
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3109/09537104.2011.596957
dc.identifier.eissn1369-1635
dc.identifier.issn0953-7104
dc.identifier.pubmedidMEDLINE:21787173
dc.identifier.urihttps://doi.org/10.3109/09537104.2011.596957
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76980
dc.identifier.wosidWOS:000297812900006
dc.information.autorucMedicina;Berkovits A ;S/I;2242
dc.information.autorucMedicina;Matus V ;S/I;1744
dc.information.autorucMedicina;Mezzano D ;S/I;99455
dc.information.autorucMedicina;Padilla O ;S/I;102063
dc.information.autorucMedicina;Panes O ;S/I;90271
dc.information.autorucMedicina;Pereira J;S/I;99371
dc.information.autorucMedicina;Saez C;S/I;102375
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final44
dc.pagina.inicio36
dc.publisherTAYLOR & FRANCIS INC
dc.revistaPLATELETS
dc.rightsacceso restringido
dc.subjectPlatelet function
dc.subjectplatelets and fibrinolysis
dc.subjecttranexamic acid
dc.subjectTHROMBIN-ACTIVATABLE FIBRINOLYSIS
dc.subjectTISSUE FACTOR EXPRESSION
dc.subjectPLASMINOGEN-ACTIVATOR
dc.subjectRISK-FACTOR
dc.subjectFACTOR-XI
dc.subjectMYOCARDIAL-INFARCTION
dc.subjectINHIBITS FIBRINOLYSIS
dc.subjectVENOUS THROMBOSIS
dc.subjectBLEEDING-TIME
dc.subjectATORVASTATIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleClot lysis time in platelet-rich plasma: Method assessment, comparison with assays in platelet-free and platelet-poor plasmas, and response to tranexamic acid
dc.typeartículo
dc.volumen23
sipa.codpersvinculados2242
sipa.codpersvinculados1744
sipa.codpersvinculados99455
sipa.codpersvinculados102063
sipa.codpersvinculados90271
sipa.codpersvinculados99371
sipa.codpersvinculados102375
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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