Pregnancy imparts distinct systemic adaptive immune function

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dc.contributor.authorDemery-Poulos, Catherine
dc.contributor.authorRomero, Roberto
dc.contributor.authorXu, Yi
dc.contributor.authorArenas-Hernandez, Marcia
dc.contributor.authorMiller, Derek
dc.contributor.authorTao, Li
dc.contributor.authorGalaz, Jose
dc.contributor.authorFarias-Jofre, Marcelo
dc.contributor.authorBhatti, Gaurav
dc.contributor.authorGarcia-Flores, Valeria
dc.contributor.authorSeyerle, Megan
dc.contributor.authorTarca, Adi L.
dc.contributor.authorGomez-Lopez, Nardhy
dc.date.accessioned2024-01-10T12:37:16Z
dc.date.available2024-01-10T12:37:16Z
dc.date.issued2022
dc.description.abstractProblem Pregnancy represents a state of systemic immune activation that is primarily driven by alterations in circulating innate immune cells. Recent studies have suggested that cellular adaptive immune components, T cells and B cells, also undergo changes throughout gestation. However, the phenotypes and functions of such adaptive immune cells are poorly understood. Herein, we utilized high-dimensional flow cytometry and functional assays to characterize T-cell and B-cell responses in pregnant and non-pregnant women. Methods Peripheral blood mononuclear cells from pregnant (n = 20) and non-pregnant (n = 25) women were used for phenotyping of T-cell and B-cell subsets. T-cell proliferation and B-cell activation were assessed by flow cytometry after in vitro stimulation, and lymphocyte cytotoxicity was evaluated by using a cell-based assay. Statistical comparisons were performed with linear mixed-effects models. Results Pregnancy was associated with modestly enhanced basal activation of peripheral CD4(+) T cells. Both CD4(+) and CD8(+) T cells from pregnant women showed increased activation-induced proliferation; yet, a reduced proportion of these cells expressed activation markers compared to non-pregnant women. There were no differences in peripheral lymphocyte cytotoxicity between study groups. A greater proportion of B cells from pregnant women displayed memory-like and activated phenotypes, and such cells exhibited higher activation following stimulation. Conclusion Maternal circulating T cells and B cells display distinct responses during pregnancy. The former may reflect the unique capacity of T cells to respond to potential threats without undergoing aberrant activation, thereby preventing systemic inflammatory responses that can lead to adverse perinatal consequences.
dc.description.funderPerinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and
dc.fechaingreso.objetodigital2024-05-28
dc.fuente.origenWOS
dc.identifier.doi10.1111/aji.13606
dc.identifier.eissn1600-0897
dc.identifier.issn1046-7408
dc.identifier.urihttps://doi.org/10.1111/aji.13606
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76801
dc.identifier.wosidWOS:000850242500001
dc.information.autorucFacultad de Medicina; Farias Jofre, Marcelo Enrique; S/I; 12286
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherWILEY
dc.revistaAMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
dc.rightsacceso restringido
dc.subjectadaptive immunity
dc.subjectB cell
dc.subjectcytotoxicity
dc.subjectflow cytometry
dc.subjectmaternal circulation
dc.subjectT cell
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titlePregnancy imparts distinct systemic adaptive immune function
dc.typeartículo
sipa.codpersvinculados12286
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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