Transcriptional repression of equilibrative nucleoside transporter 1 by D-glucose involves nitric oxide and Sp1 in human foetal endothelium

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dc.catalogadorpau
dc.contributor.authorPuebla Aracena, Carlos Alberto
dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorVecchiola Cárdenas, Andrea Paola
dc.contributor.authorCasanello Toledo, Paola Cecilia
dc.contributor.authorSobrevía Luarte, Luis Alberto
dc.date.accessioned2023-07-21T17:46:17Z
dc.date.available2023-07-21T17:46:17Z
dc.date.issued2007
dc.description.abstractAdenosine uptake via the human equilibrative nucleoside transporters 1 (hENT1) is reduced in human umbilical vein endothelial cells (HUVEC) exposed to high D-glucose. Since specific mechanisms by which D-glucose reduces hENT1 expression and activity in these cells are unknown, we examined the role of nitric oxide (NO) and Sp1 as modulators of SLC29A1 (for hENT1) promoter activity in response to D-glucose. HUVEC from normal pregnancies were isolated and exposed (24 h) to 5 mM (normal) or 25 mM (high) D-glucose in absence or presence of NG-nitro-L-arginine methyl ester (LNAME, 100 mM). Sp1 protein levels were evaluated by western blot in nuclear fractions. Reporter activity of plasmid constructs containing a promoter region of SLC29A1 (-1114 bp to ATG, pGL3-hENT1-1114), Sp1 overexpression experiments (co-transfection with the expression vector pCGN-Sp1) and Sp1 binding to this region (chromatin immunoprecipitation) was assayed. pGL3-hENT1-1114 transcriptional activity was reduced by high Dglucose and in cells overexpressing Sp1. Nuclear Sp1 abundance and specific binding to its consensus sequence in SLC29A1 promoter (-815 to -801 bp) were higher in high D-glucose (1.9- and 1.7-fold, respectively) compared with normal D-glucose. All these effects were blocked by L-NAME. Thus, a NO-dependent Sp1 repression on SLC29A1 promoter could explain the reduced hENT1 expression and activity exhibited by HUVEC in high extracellular D-glucose. FONDECYT 1070865, VRAID2007-PUC (Chile). AECI A/5484/06 (Spain). C. Puebla holds a PUC-VRAID PhD fellowship. M. Farías hold CONICYT and PUC-School of Medicine PhD fellowships
dc.format.extent1 página
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.placenta.2007.06.001
dc.identifier.issn0143-4004
dc.identifier.urihttps://publons.com/wos-op/publon/18766750/
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74285
dc.information.autorucFacultad de Ciencias Biológicas ; Puebla Aracena, Carlos Alberto ; 0000-0001-6648-2135 ; 124965
dc.information.autorucEscuela de Medicina ; Farías Jofré, Marcelo Enrique ; 0000-0003-0473-2295 ; 12286
dc.information.autorucEscuela de Medicina ; Vecchiola Cárdenas, Andrea Paola ; S/I ; 123319
dc.information.autorucEscuela de Medicina ; Casanello Toledo, Paola Cecilia ; 0000-0002-2355-1476 ; 146772
dc.information.autorucEscuela de Medicina ; Sobrevía Luarte, Luis Alberto ; 0000-0001-5802-2243 ; 1002656
dc.issue.numero8-9
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.finalA24
dc.pagina.inicioA24
dc.relation.ispartofInternational Federation of Placenta Associations Meetings (13° ; 2007 ; Ontario, Canada)
dc.revistaPlacentaes_ES
dc.rightsacceso restringido
dc.titleTranscriptional repression of equilibrative nucleoside transporter 1 by D-glucose involves nitric oxide and Sp1 in human foetal endotheliumes_ES
dc.typecomunicación de congreso
dc.volumen28
sipa.codpersvinculados124965
sipa.codpersvinculados12286
sipa.codpersvinculados123319
sipa.codpersvinculados146772
sipa.codpersvinculados1002656
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