Microribonucleic acid-21 increases aldosterone secretion and proliferation in H295R human adrenocortical cells

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dc.contributor.authorRomero, Damian G.
dc.contributor.authorPlonczynski, Maria W.
dc.contributor.authorCarvajal, Cristian A.
dc.contributor.authorGomez Sanchez, Elise P.
dc.contributor.authorGomez Sanchez, Celso E.
dc.date.accessioned2024-01-10T13:50:31Z
dc.date.available2024-01-10T13:50:31Z
dc.date.issued2008
dc.description.abstractMicroRNAs (miRNAs) are endogenous small noncoding RNAs that decrease the expression levels of specific genes by translational repression, sequestration, and degradation of their mRNAs. Angiotensin II is an important modulator of adrenal zona glomerulosa cell physiology, including steroidogenesis and proliferation among many other physiological processes. Because each miRNA may regulate the expression levels of multiple genes, thereby resembling the transcription regulatory networks triggered by transcription factors, we hypothesize that specific miRNAs may be involved in angiotensin II-mediated adrenocortical cell physiology. The human adrenocortical cell line H295R is the only adrenal cell line available with a steroid secretion pattern and regulation similar to freshly isolated adrenocortical cells. We screened for miRNAs regulated by angiotensin II in H295R cells and found that miRNA-21 expression levels were specifically modulated by angiotensin II. Angiotensin II time dependently increased miRNA-21 expression reaching a 4.4-fold induction after 24 h. Angiotensin II-mediated miRNA-21 expression resulted in biologically active miRNA-21, determined using a fusion mRNA reporter system carrying miRNA-21 target sequences in its 3' untranslated region. Up-regulation of miRNA-21 intracellular levels increased aldosterone secretion but not cortisol. Elevation of miRNA-21 levels also increased cell proliferation in H295R cells. In summary, miRNA-21 is an endogenously expressed miRNA in human adrenal cells. miRNA-21 expression is up-regulated by angiotensin II, and its overexpression caused an increase in aldosterone secretion and cell proliferation. Alterations in miRNA-21 expression levels or function may be involved in dysregulation of angiotensin II signaling and abnormal aldosterone secretion by adrenal glands in humans.
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.description.funderNHLBI NIH HHS
dc.fechaingreso.objetodigital2024-05-15
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1210/en.2007-1686
dc.identifier.eissn1945-7170
dc.identifier.issn0013-7227
dc.identifier.pubmedidMEDLINE:18218696
dc.identifier.urihttps://doi.org/10.1210/en.2007-1686
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79536
dc.identifier.wosidWOS:000255200000048
dc.information.autorucMedicina;Carvajal C;S/I;8586
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final2483
dc.pagina.inicio2477
dc.publisherENDOCRINE SOC
dc.revistaENDOCRINOLOGY
dc.rightsacceso restringido
dc.subjectVASCULAR INJURY
dc.subjectGENE-REGULATION
dc.subjectBLOOD-PRESSURE
dc.subjectSMALL RNAS
dc.subjectMICRO-RNA
dc.subjectHYPERTENSION
dc.subjectMICRORNA-21
dc.subjectMIRNAS
dc.subjectMODEL
dc.subjectINVOLVEMENT
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMicroribonucleic acid-21 increases aldosterone secretion and proliferation in H295R human adrenocortical cells
dc.typeartículo
dc.volumen149
sipa.codpersvinculados8586
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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