Isolation of Ciprofloxacin and Ceftazidime-Resistant Enterobacterales From Vegetables and River Water Is Strongly Associated With the Season and the Sample Type

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dc.contributor.authorDiaz Gavidia, Constanza
dc.contributor.authorBarria, Carla
dc.contributor.authorRivas, Lina
dc.contributor.authorGarcia, Patricia
dc.contributor.authorAlvarez, Francisca P.
dc.contributor.authorGonzalez Rocha, Gerardo
dc.contributor.authorOpazo Capurro, Andres
dc.contributor.authorAraos, Rafael
dc.contributor.authorMunita, Jose M.
dc.contributor.authorCortes, Sandra
dc.contributor.authorOlivares Pacheco, Jorge
dc.contributor.authorAdell, Aiko D.
dc.contributor.authorMoreno Switt, Andrea I.
dc.date.accessioned2024-01-10T13:44:52Z
dc.date.available2024-01-10T13:44:52Z
dc.date.issued2021
dc.description.abstractThe dissemination of antibiotic-resistant bacteria (ARB) from water used for crop irrigation to vegetables is poorly studied. During a year, five farmer markets in a city in Central Chile were visited, and 478 vegetable samples (parsleys, corianders, celeries, lettuces, chards, and beets) were collected. Simultaneously, 32 water samples were collected from two rivers which are used to irrigate the vegetables produced in the area. Resistant Enterobacterales were isolated and identified. Colistin resistance gene mcr-1 and extended spectrum beta-lactamases (ESBL) were molecularly detected. The association of environmental factors was evaluated, with the outcomes being the presence of Enterobacterales resistant to four antibiotic families and the presence of multidrug resistance (MDR) phenotypes. Parsley, coriander, and celery showed the highest prevalence of resistant Enterobacterales (41.9% for ciprofloxacin and 18.5% for ceftazidime). A total of 155 isolates were obtained, including Escherichia coli (n = 109), Citrobacter sp. (n = 20), Enterobacter cloacae complex (n = 8), Klebsiella pneumoniae (n = 8), and Klebsiella aerogenes (n = 1). Resistance to ampicillin (63.2%) and ciprofloxacin (74.2%) was most frequently found; 34.5% of the isolates showed resistance to third-generation cephalosporins, and the MDR phenotype represented 51.6% of the isolates. In two E. coli isolates (1.29%), the gene mcr-1 was found and ESBL genes were found in 23/62 isolates (37%), with bla(CTX-M) being the most frequently found in 20 isolates (32%). Resistant Enterobacterales isolated during the rainy season were less likely to be MDR as compared to the dry season. Understanding environmental associations represent the first step toward an improved understanding of the public health impact of ARB in vegetables and water.
dc.description.funderFONDECYT
dc.description.funderANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance, MICROB-R
dc.description.funderFONDAP-ACCDIS grant
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent14 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3389/fmicb.2021.604567
dc.identifier.eissn1664-302X
dc.identifier.pubmedidMEDLINE:34594307
dc.identifier.urihttps://doi.org/10.3389/fmicb.2021.604567
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78949
dc.identifier.wosidWOS:000733810400001
dc.information.autorucFacultad de Agronomía e Ingeniería Forestal; Moreno Switt, Andrea Isabel; S/I; 1147061
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherFRONTIERS MEDIA SA
dc.revistaFRONTIERS IN MICROBIOLOGY
dc.rightsacceso abierto
dc.subjectmultidrug resistance
dc.subjectvegetable
dc.subjectriver water
dc.subjectChile
dc.subjectenvironmental risk factors
dc.subjectEnterobacterales
dc.subjectSPECTRUM-BETA-LACTAMASE
dc.subjectESCHERICHIA-COLI
dc.subjectANTIBIOTIC-RESISTANCE
dc.subjectFRESH VEGETABLES
dc.subjectPREVALENCE
dc.subjectCONTAMINATION
dc.subjectANIMALS
dc.subjectHEALTHY
dc.subjectSPAIN
dc.subjectSOIL
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIsolation of Ciprofloxacin and Ceftazidime-Resistant Enterobacterales From Vegetables and River Water Is Strongly Associated With the Season and the Sample Type
dc.typeartículo
dc.volumen12
sipa.codpersvinculados1147061
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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