Differences in berry primary and secondary metabolisms identified by transcriptomic and metabolic profiling of two table grape color somatic variants

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dc.contributor.authorSantibáñez, Claudia
dc.contributor.authorMeyer Regueiro, Carlos José
dc.contributor.authorMartínez, Litsy
dc.contributor.authorMoyano, Tomás
dc.contributor.authorLunn, John
dc.contributor.authorFeil, Regina
dc.contributor.authorDai, Zhanwu
dc.contributor.authorCarrasco, David
dc.contributor.authorArroyo García, Rosa
dc.contributor.authorHilbert,Ghislaine
dc.contributor.authorRenaud, Christel
dc.contributor.authorDelrot, Serge
dc.contributor.authorManke Nachtigall, Fabiane
dc.contributor.authorGutiérrez Ilabaca, Rodrigo Antonio
dc.contributor.authorMatus, José Tomás
dc.contributor.authorGomès, Eric
dc.contributor.authorArce Johnson, Jorge Patricio
dc.date.accessioned2023-06-02T19:55:12Z
dc.date.available2023-06-02T19:55:12Z
dc.date.issued2019
dc.description.abstractAnthocyanins are flavonoids responsible for the color of berries in skin-pigmented grapevine (Vitis vinifera L.). Due to the widely adopted vegetative propagation of this species, somatic mutations occurring in meristematic cell layers can be fixed and passed into the rest of the plant when cloned. In this study we focused on the transcriptomic and metabolic differences between two color somatic variants. Using microscopic, metabolic and mRNA profiling analyses we compared the table grape cultivar (cv.) ‘Red Globe’ (RG, with purplish berry skin) and cv. ‘Chimenti Globe’ (CG, with a contrasting reddish berry skin color). As expected, significant differences were found in the composition of flavonoids and other phenylpropanoids, but also in their upstream precursors’ shikimate and phenylalanine. Among primary metabolites, sugar phosphates related with sucrose biosynthesis were less accumulated in cv. ‘CG’. The red-skinned cv. ‘CG’ only contained di-hydroxylated anthocyanins (i.e. peonidin and cyanidin) while the tri-hydroxylated derivatives malvidin, delphinidin and petunidin were absent, in correlation to the reddish cv. ‘CG’ skin coloration. Transcriptomic analysis showed alteration in flavonoid metabolism and terpenoid pathways and in primary metabolism such as sugar content. Eleven flavonoid 3’5’-hydroxylase gene copies were down-regulated in cv. ‘CG’. This family of cytochrome P450 oxidoreductases are key in the biosynthesis of tri-hydroxylated anthocyanins. Many transcription factors appeared down-regulated in cv. ‘CG’ in correlation to the metabolic and transcriptomic changes observed. The use of molecular markers and its confirmation with our RNA-seq data showed the exclusive presence of the null MYBA2 white allele (i.e. homozygous in both L1 and L2 layers) in the two somatic variants. Therefore, the differences in MYBA1 expression seem sufficient for the skin pigmentation differences and the changes in MYBA target gene expression in cv. ‘Chimenti Globe’.
dc.fechaingreso.objetodigital2023-06-02
dc.fuente.origenORCID
dc.identifier.doi10.1101/861120
dc.identifier.urihttp://doi.org/10.1101/861120
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/70514
dc.information.autorucFacultad de Ciencias Biológicas;Gutiérrez Ilabaca Rodrigo Antonio;0000-0002-5961-5005;86782
dc.information.autorucFacultad de Ciencias Biológicas; Arce Johnson, Jorge Patricio;s/i;54718
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleDifferences in berry primary and secondary metabolisms identified by transcriptomic and metabolic profiling of two table grape color somatic variants
dc.typepreprint
sipa.codpersvinculados86782
sipa.codpersvinculados54718
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