Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes

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dc.contributor.authorPrieto Hontoria, Pedro L.
dc.contributor.authorPerez Matute, Patricia
dc.contributor.authorFernandez Galilea, Marta
dc.contributor.authorLopez Yoldi, Miguel
dc.contributor.authorSinal, Christopher J.
dc.contributor.authorAlfredo Martinez, J.
dc.contributor.authorMoreno Aliaga, Maria J.
dc.date.accessioned2024-01-10T12:38:34Z
dc.date.available2024-01-10T12:38:34Z
dc.date.issued2016
dc.description.abstractChemerin is a novel adipokine associated with obesity and insulin resistance. a-Lipoic acid (alpha-LA) has shown beneficial properties on diabetes and obesity. The aim of this study was to examine the effects of alpha-LA on chemerin production in adipocytes in absence or presence of TNF-alpha, insulin and AICAR. The potential signaling pathways involved in alpha-LA effects on chemerin were also analyzed. alpha-LA actions on chemerin were tested in differentiated 3T3-L1 adipocytes and in some cases in human subcutaneous and omental adipocytes. Chemerin mRNA levels were measured by RT-PCR and the amount of chemerin secreted to culture media was determined by ELISA. alpha-LA induced a concentration-dependent inhibition on both chemerin secretion and mRNA levels in 3T3-L1 adipocytes. The AMPK activator AICAR and the PI3K inhibitor LY294002 dramatically abrogated both chemerin secretion and gene expression, and further potentiated the inhibitory effect of alpha-LA on chemerin secretion. Insulin was able to partially reverse the inhibitory action of alpha-LA on chemerin secretion. alpha-LA also reduced basal chemerin secretion in both subcutaneous and omental adipocytes from overweight/obese subjects. Moreover, alpha-LA was able to abolish the stimulatory effects of the pro-inflammatory cytokine TNF-alpha on chemerin secretion. Our data demonstrated the ability of alpha-LA to inhibit chemerin production, an adipokine associated to obesity and metabolic syndrome, suggesting that the reduction of chemerin could contribute to the antiobesity/antidiabetic properties described for alpha-LA. 2015 Elsevier B.V. All rights reserved.
dc.description.funderMinistry of Science and Innovation of the Government of Spain (AGL)
dc.description.funderLinea Especial: "Nutricion, Obesidad y Salud" (University of Navarra)
dc.description.funderCanadian Institutes of Health Research
dc.description.funderInstituto Danone, Spain
dc.description.funderNavarra Goverment
dc.description.funderAsociacion de Amigos de la Universidad de Navarra
dc.fechaingreso.objetodigital20-03-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.bbalip.2015.12.011
dc.identifier.eissn0006-3002
dc.identifier.issn1388-1981
dc.identifier.pubmedidMEDLINE:26721419
dc.identifier.urihttps://doi.org/10.1016/j.bbalip.2015.12.011
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77065
dc.identifier.wosidWOS:000370097300012
dc.information.autorucMedicina; Fernandez M;S/I;242757
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final268
dc.pagina.inicio260
dc.publisherELSEVIER SCIENCE BV
dc.revistaBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
dc.rightsacceso restringido
dc.subjectLipoic acid
dc.subjectObesity
dc.subjectChemerin
dc.subjectAdipocytes
dc.subjectACTIVATED PROTEIN-KINASE
dc.subjectINDUCED INSULIN-RESISTANCE
dc.subjectHIGH-FAT DIET
dc.subjectMETABOLIC SYNDROME
dc.subjectSIGNALING PATHWAY
dc.subjectGENE-EXPRESSION
dc.subjectLEPTIN SECRETION
dc.subjectADIPOSE-TISSUE
dc.subjectRAT ADIPOCYTES
dc.subjectGLUCOSE-INTOLERANCE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes
dc.typeartículo
dc.volumen1861
sipa.codpersvinculados242757
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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