Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin

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dc.contributor.authorAmaya, Fernando A.
dc.contributor.authorBlondel, Carlos J.
dc.contributor.authorBarros Infante, Maria F.
dc.contributor.authorRivera, Dacil
dc.contributor.authorMoreno Switt, Andrea I.
dc.contributor.authorSantiviago, Carlos A.
dc.contributor.authorPezoa, David
dc.date.accessioned2024-01-10T12:37:00Z
dc.date.available2024-01-10T12:37:00Z
dc.date.issued2022
dc.description.abstractThe Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). In Salmonella, five T6SS gene clusters have been identified within pathogenicity islands SPI-6, SPI-19, SPI-20, SPI-21, and SPI-22, which are differentially distributed among serotypes. Salmonella enterica serotype Dublin (S. Dublin) is a cattle-adapted pathogen that harbors both T6SS(SPI-6) and T6SS(SPI-19). Interestingly, while both systems have been linked to virulence and host colonization in S. Dublin, an antibacterial activity has not been detected for T6SS(SPI-6) in this serotype. In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS(SPI-6) and T6SS(SPI-19) gene clusters in S. Dublin. In the present study, we demonstrate that T6SS(SPI-6) and T6SS(SPI-19) of S. Dublin CT_02021853 contribute to interbacterial competition. Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. Finally, we demonstrate that E/I modules SED_RS01930/SED_RS01935 (encoded in SPI-6), SED_RS06235/SED_RS06230, and SED_RS06335/SED_RS06340 (both encoded in SPI-19) contribute to interbacterial competition in S. Dublin CT_02021853.
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3389/fmicb.2022.811932
dc.identifier.eissn1664-302X
dc.identifier.pubmedidMEDLINE:35222335
dc.identifier.urihttps://doi.org/10.3389/fmicb.2022.811932
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76721
dc.identifier.wosidWOS:000760809700001
dc.information.autorucFacultad de Agronomía e Ingeniería Forestal; Moreno Switt, Andrea Isabel; S/I; 1147061
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherFRONTIERS MEDIA SA
dc.revistaFRONTIERS IN MICROBIOLOGY
dc.rightsacceso abierto
dc.subjectSalmonella Dublin
dc.subjectinterbacterial competition
dc.subjectT6SS
dc.subjecteffector
dc.subjectimmunity protein
dc.subjectESCHERICHIA-COLI
dc.subjectRHS PROTEINS
dc.subjectANTIBACTERIAL
dc.subjectSEQUENCE
dc.subjectTOXIN
dc.subjectREQUIRES
dc.subjectDOMAIN
dc.subjectVGRG
dc.subjectPAAR
dc.subjectSPECIFICITY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIdentification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
dc.typeartículo
dc.volumen13
sipa.codpersvinculados1147061
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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