Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon

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dc.contributor.authorTerry Lorenzo, Ryan T.
dc.contributor.authorTorres, Viviana I.
dc.contributor.authorWagh, Dhananjay
dc.contributor.authorGalaz, Jose
dc.contributor.authorSwanson, Selene K.
dc.contributor.authorFlorens, Laurence
dc.contributor.authorWashburn, Michael P.
dc.contributor.authorWaites, Clarissa L.
dc.contributor.authorGundelfinger, Eckart D.
dc.contributor.authorReimer, Richard J.
dc.contributor.authorGarner, Craig C.
dc.date.accessioned2024-01-10T13:44:02Z
dc.date.available2024-01-10T13:44:02Z
dc.date.issued2016
dc.description.abstractSynaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the pre synaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis.
dc.description.funderNIH
dc.description.funderDeutsches Zentrum fur Neurodegenerative Erkrankung (DZNE)
dc.description.funderDeutsche Forschungsgemeinschaft
dc.description.funderMax Planck Award of the Alexander von Humboldt Foundation/Max Planck Society
dc.description.funderStowers Institute for Medical Research
dc.format.extent29 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pone.0167535
dc.identifier.issn1932-6203
dc.identifier.pubmedidMEDLINE:27907191
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0167535
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78821
dc.identifier.wosidWOS:000389482700187
dc.information.autorucCiencias Biológicas;Torres V;S/I;1025347
dc.issue.numero12
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherPUBLIC LIBRARY SCIENCE
dc.revistaPLOS ONE
dc.rightsregistro bibliográfico
dc.subjectSYNAPTIC VESICLE EXOCYTOSIS
dc.subjectNUCLEOTIDE-EXCHANGE FACTORS
dc.subjectDEVELOPING RAT-BRAIN
dc.subjectIDENTIFICATION TECHNOLOGY
dc.subjectNEUROTRANSMITTER RELEASE
dc.subjectEXCITATORY SYNAPSES
dc.subjectHIPPOCAMPAL-NEURONS
dc.subjectSHOTGUN PROTEOMICS
dc.subjectPRECURSOR VESICLES
dc.subjectNEURITE OUTGROWTH
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTrio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon
dc.typeartículo
dc.volumen11
sipa.codpersvinculados1025347
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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