A Deficiency in the Flavoprotein of Arabidopsis Mitochondrial Complex II Results in Elevated Photosynthesis and Better Growth in Nitrogen-Limiting Conditions

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dc.contributor.authorFuentes, Daniela
dc.contributor.authorMeneses, Marco
dc.contributor.authorNunes Nesi, Adriano
dc.contributor.authorAraujo, Wagner L.
dc.contributor.authorTapia, Rodrigo
dc.contributor.authorGomez, Isabel
dc.contributor.authorHoluigue, Loreto
dc.contributor.authorGutierrez, Rodrigo A.
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorJordana, Xavier
dc.date.accessioned2024-01-10T13:45:37Z
dc.date.available2024-01-10T13:45:37Z
dc.date.issued2011
dc.description.abstractMitochondrial complex II (succinate dehydrogenase [SDH]) plays roles both in the tricarboxylic acid cycle and the respiratory electron transport chain. In Arabidopsis (Arabidopsis thaliana), its flavoprotein subunit is encoded by two nuclear genes, SDH1-1 and SDH1-2. Here, we characterize heterozygous SDH1-1/sdh1-1 mutant plants displaying a 30% reduction in SDH activity as well as partially silenced plants obtained by RNA interference. We found that these plants displayed significantly higher CO2 assimilation rates and enhanced growth than wild-type plants. There was a strong correlation between CO2 assimilation and stomatal conductance, and both mutant and silenced plants displayed increased stomatal aperture and density. By contrast, no significant differences were found for dark respiration, chloroplastic electron transport rate, CO2 uptake at saturating concentrations of CO2, or biochemical parameters such as the maximum rates of carboxylation by Rubisco and of photosynthetic electron transport. Thus, photosynthesis is enhanced in SDH-deficient plants by a mechanism involving a specific effect on stomatal function that results in improved CO2 uptake. Metabolic and transcript profiling revealed that mild deficiency in SDH results in limited effects on metabolism and gene expression, and data suggest that decreases observed in the levels of some amino acids were due to a higher flux to proteins and other nitrogen-containing compounds to support increased growth. Strikingly, SDH1-1/sdh1-1 seedlings grew considerably better in nitrogen-limiting conditions. Thus, a subtle metabolic alteration may lead to changes in important functions such as stomatal function and nitrogen assimilation.
dc.description.funderFondecyt-Chile
dc.description.funderMillennium Nucleus for Plant Functional Genomics
dc.description.funderMillennium Scientific Initiative
dc.description.funderMideplan, Chile
dc.description.funderMax Planck Gesellschaft
dc.description.funderGerman Research Council
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent14 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1104/pp.111.183939
dc.identifier.issn0032-0889
dc.identifier.pubmedidMEDLINE:21921116
dc.identifier.urihttps://doi.org/10.1104/pp.111.183939
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79056
dc.identifier.wosidWOS:000296722300012
dc.information.autorucCiencias Biológicas;Fuentes D ;S/I;121669
dc.information.autorucCiencias Biológicas;Gutierrez R;S/I;86782
dc.information.autorucCiencias Biológicas;Holuigue L ;S/I;50349
dc.information.autorucCiencias Biológicas;Jordana X;S/I;99673
dc.information.autorucCiencias Biológicas;Meneses M ;S/I;172003
dc.information.autorucCiencias Biológicas;Tapia R ;S/I;120375
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final1127
dc.pagina.inicio1114
dc.publisherAMER SOC PLANT BIOLOGISTS
dc.revistaPLANT PHYSIOLOGY
dc.rightsacceso abierto
dc.subjectIRON-SULFUR SUBUNIT
dc.subjectSUCCINATE-DEHYDROGENASE
dc.subjectCARBON-DIOXIDE
dc.subjectNUCLEAR GENE
dc.subjectENHANCES PHOTOSYNTHESIS
dc.subjectNITRATE ASSIMILATION
dc.subjectNICOTIANA-SYLVESTRIS
dc.subjectILLUMINATED LEAVES
dc.subjectREDUCED EXPRESSION
dc.subjectRESPIRATORY-CHAIN
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleA Deficiency in the Flavoprotein of Arabidopsis Mitochondrial Complex II Results in Elevated Photosynthesis and Better Growth in Nitrogen-Limiting Conditions
dc.typeartículo
dc.volumen157
sipa.codpersvinculados121669
sipa.codpersvinculados86782
sipa.codpersvinculados50349
sipa.codpersvinculados99673
sipa.codpersvinculados172003
sipa.codpersvinculados120375
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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