beta-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle

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dc.contributor.authorMonsalves Alvarez, Matias
dc.contributor.authorMorales, Pablo Esteban
dc.contributor.authorCastro Sepulveda, Mauricio
dc.contributor.authorSepulveda, Carlos
dc.contributor.authorRodriguez, Juan Manuel
dc.contributor.authorChiong, Mario
dc.contributor.authorEisner, Veronica
dc.contributor.authorLavandero, Sergio
dc.contributor.authorTroncoso, Rodrigo
dc.date.accessioned2024-01-10T14:22:31Z
dc.date.available2024-01-10T14:22:31Z
dc.date.issued2020
dc.description.abstractbeta-hydroxybutyrate is the main ketone body generated by the liver under starvation. Under these conditions, it can sustain ATP levels by its oxidation in mitochondria. As mitochondria can modify its shape and function under different nutritional challenges, we study the chronic effects of beta-hydroxybutyrate supplementation on mitochondrial morphology and function, and its relation to exercise capacity. Male C57BL/6 mice were supplemented with beta-hydroxybutyrate mineral salt (3.2%) or control (CT, NaCl/KCl) for six weeks and submitted to a weekly exercise performance test. We found an increase in distance, maximal speed, and time to exhaustion at two weeks of supplementation. Fatty acid metabolism and OXPHOS subunit proteins declined at two weeks in soleus but not in tibialis anterior muscles. Oxygen consumption rate on permeabilized fibers indicated a decrease in the presence of pyruvate in the short-term treatment. Both the tibialis anterior and soleus showed decreased levels of Mitofusin 2, while electron microscopy assessment revealed a significant reduction in mitochondrial cristae shape in the tibialis anterior, while a reduction in the mitochondrial number was observed only in soleus. These results suggest that short, but not long-term, beta-hydroxybutyrate supplementation increases exercise capacity, associated with modifications in mitochondrial morphology and function in mouse skeletal muscle.
dc.description.funderFondo Nacional de Desarrollo Cientifico y Tecnologico, FONDECYT
dc.description.funderPIA
dc.description.funderFONDAP
dc.description.funderUniversity of Chile
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent18 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3390/nu12071930
dc.identifier.eissn2072-6643
dc.identifier.pubmedidMEDLINE:32610627
dc.identifier.urihttps://doi.org/10.3390/nu12071930
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79949
dc.identifier.wosidWOS:000557867400001
dc.information.autorucFacultad de Ciencias Biológicas; Eisner Sagues, Veronica Raquel; S/I; 238175
dc.issue.numero7
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI
dc.revistaNUTRIENTS
dc.rightsacceso abierto
dc.subjectketone bodies
dc.subjectbeta-hydroxybutyrate
dc.subjectmitochondrial morphology
dc.subjectskeletal muscle
dc.subjectendurance
dc.subjectKETONE-BODIES
dc.subjectOXIDATIVE STRESS
dc.subjectPERFORMANCE
dc.subjectDYNAMICS
dc.subjectFUSION
dc.subjectMODEL
dc.subjectDEGRADATION
dc.subjectBIOGENESIS
dc.subjectDEFICIENCY
dc.subjectMETABOLISM
dc.subject.ods03 Good Health and Well-being
dc.subject.ods02 Zero Hunger
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa02 Hambre cero
dc.titlebeta-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle
dc.typeartículo
dc.volumen12
sipa.codpersvinculados238175
sipa.indexWOS
sipa.indexPubmed
sipa.trazabilidadCarga SIPA;09-01-2024
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