Role of plasma and liver cholesterol- and lipoprotein-metabolism determinants in LpX formation in the mouse

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dc.contributor.authorBravo, Ignacio
dc.contributor.authorAmigo, Ludwig
dc.contributor.authorCohen, David E.
dc.contributor.authorNervi, Flavio
dc.contributor.authorRigotti, Attilio
dc.contributor.authorFrancone, Omar
dc.contributor.authorZanlungo, Silvana
dc.date.accessioned2024-01-10T12:42:14Z
dc.date.available2024-01-10T12:42:14Z
dc.date.issued2007
dc.description.abstractCholestasis is characterized by hypercholesterolemia and the appearance of an abnormal lipoprotein, lipoprotein X (LpX), in plasma. The mechanisms responsible for this cholestatic plasma lipid phenotype are not fully understood. We used ATP-binding cassette A1 (ABCA1)((-/-)) and scavenger receptor class B type I (SR-BI)((-/-)) mice to test the hypothesis that hepatic sinusoidal cholesterol transporters contribute to LpX formation and hypercholesterolemia during cholestasis. Bile-duct ligation (BDL) of both ABCA1((-/-)) and SR-BI((-/-)) mice, as well as their respective controls, induced a dramatic increase in plasma cholesterol and phospholipid concentrations. Plasma fractionation revealed the presence of LpX in plasma of cholestatic mice, irrespective of their genetic background. We observed that the presence of HDL before cholestasis, a decrease in the activity of LCAT, and an increase in VLDL synthesis were not required for hypercholesterolemia and lipoprotein modifications induced by obstructive cholestasis in mice. In addition, murine cholestasis resulted in increased hepatic cholesterol synthesis that may contribute to the higher plasma free cholesterol levels found during the early hours after BDL. Together these findings indicate that hypercholesterolemia and LpX formation associated with obstructive cholestasis are correlated with an increase in hepatic cholesterol synthesis and are independent of plasma HDL levels, LCAT activity, VLDL synthesis, and ABCA1 and SR-BI expression. (c) 2007 Elsevier B.V. All rights reserved.
dc.fechaingreso.objetodigital20-03-2024
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.bbagen.2007.02.010
dc.identifier.issn0304-4165
dc.identifier.pubmedidMEDLINE:17399905
dc.identifier.urihttps://doi.org/10.1016/j.bbagen.2007.02.010
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77494
dc.identifier.wosidWOS:000246654000016
dc.information.autorucMedicina;Nervi F;S/I;99156
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.information.autorucMedicina;Zanlungo S;S/I;72650
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final988
dc.pagina.inicio979
dc.publisherELSEVIER SCIENCE BV
dc.revistaBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
dc.rightsacceso restringido
dc.subjectcholestasis
dc.subjecthypercholesterolemia
dc.subjectlipoprotein X
dc.subjectPREGNANE-X-RECEPTOR
dc.subjectCONSTITUTIVE ANDROSTANE RECEPTOR
dc.subjectLOW-DENSITY LIPOPROTEINS
dc.subjectBILE-DUCT LIGATION
dc.subjectB-TYPE-I
dc.subjectSCAVENGER-RECEPTOR
dc.subjectSR-BI
dc.subjectACYLTRANSFERASE DEFICIENCY
dc.subjectPHOSPHOLIPID-COMPOSITION
dc.subjectABCA1 TRANSPORTER
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRole of plasma and liver cholesterol- and lipoprotein-metabolism determinants in LpX formation in the mouse
dc.typeartículo
dc.volumen1770
sipa.codpersvinculados99156
sipa.codpersvinculados68489
sipa.codpersvinculados72650
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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