Sterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice

Simple item page
dc.contributor.authorZanlungo, S
dc.contributor.authorAmigo, L
dc.contributor.authorMendoza, H
dc.contributor.authorMiquel, JF
dc.contributor.authorVio, C
dc.contributor.authorGlick, JM
dc.contributor.authorRodriguez, A
dc.contributor.authorKozarsky, K
dc.contributor.authorQuinones, V
dc.contributor.authorRigotti, A
dc.contributor.authorNervi, F
dc.date.accessioned2024-01-10T13:43:39Z
dc.date.available2024-01-10T13:43:39Z
dc.date.issued2000
dc.description.abstractBackground & Aims: Sterol carrier protein 2 (SCP-2) enhances sterol cycling and facilitates cholesterol translocation between intracellular organelles and plasma membrane in cultured cells, including hepatocytes. We examined the role of SCP-2 in hepatic cholesterol and lipid trafficking through the sinusoidal and canalicular secretory pathways of the liver in vivo. Methods: Recombinant adenovirus-mediated SCP-2 gene transfer was used to obtain hepatic overexpression of SCP-2 in C57BL/6 mice. Results: SCP-2 overexpression in the mouse liver resulted in an 8-fold increase of SCP-2 protein levels and determined various effects on lipid metabolism. It decreased high-density lipoprotein cholesterol and increased low-density lipoprotein (LDL) cholesterol concentrations. The expressions of hepatic LDL receptor, apolipoprotein (apo) A-I, apoB, and apoE were decreased. SCP-2 overexpression also increased hepatic cholesterol concentration, associated with decreased cholesterol neosynthesis. Increased biliary cholesterol and bile acid secretion, bile acid pool size, and intestinal cholesterol absorption were also observed. Conclusions: These results indicate that modulation of SCP-2 expression in the liver determines important modifications on lipoprotein metabolism, hepatic cholesterol synthesis and storage, biliary lipid secretion, bile acid metabolism and intestinal cholesterol absorption.
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.description.funderNHLBI NIH HHS
dc.fechaingreso.objetodigital2024-05-02
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1053/gast.2000.20198
dc.identifier.issn0016-5085
dc.identifier.pubmedidMEDLINE:11113092
dc.identifier.urihttps://doi.org/10.1053/gast.2000.20198
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78710
dc.identifier.wosidWOS:000165833800034
dc.information.autorucMedicina;Miquel J;S/I;72216
dc.information.autorucMedicina;Nervi F;S/I;99156
dc.information.autorucMedicina;Rigotti A;S/I;68489
dc.information.autorucCiencias Biológicas;Vio C;S/I;99575
dc.information.autorucMedicina;Zanlungo S;S/I;72650
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1719
dc.pagina.inicio1708
dc.publisherW B SAUNDERS CO
dc.revistaGASTROENTEROLOGY
dc.rightsacceso restringido
dc.subjectDENSITY-LIPOPROTEIN RECEPTOR
dc.subjectBILE-ACID SYNTHESIS
dc.subjectBILIARY CHOLESTEROL SECRETION
dc.subjectRAT HEPATOCYTES
dc.subjectSR-BI
dc.subjectMEMBRANE
dc.subjectTRANSPORT
dc.subjectBIOSYNTHESIS
dc.subjectHYPERCHOLESTEROLEMIA
dc.subjectOVEREXPRESSION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice
dc.typeartículo
dc.volumen119
sipa.codpersvinculados72216
sipa.codpersvinculados99156
sipa.codpersvinculados68489
sipa.codpersvinculados99575
sipa.codpersvinculados72650
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Sterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice.pdf
Size:
2.83 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas