The absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice.

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dc.catalogadorgjm
dc.contributor.authorBerkowitz Fiebich, Loni
dc.contributor.authorPardo Roa, Catalina
dc.contributor.authorRamírez Rojas, Gigliola
dc.contributor.authorVallejos Galvez, Omar Patricio
dc.contributor.authorSebastián Quijada, Valentina Pilar
dc.contributor.authorRiedel, Claudia A.
dc.contributor.authorÁlvarez Lobos, Manuel
dc.contributor.authorBueno Ramírez, Susan
dc.date.accessioned2024-01-30T15:23:30Z
dc.date.available2024-01-30T15:23:30Z
dc.date.issued2019
dc.description.abstractPaneth cells (PCs) are specialized epithelial cells of the small bowel that contain multiple secretory granules filled with antimicrobial peptides and trophic factors, which are essential for the control of the microorganisms growth and maintaining intestinal integrity. Alterations in their function are associated with an imbalance of the normal microbiota, gastrointestinal infections and inflammatory processes, such as Crohn's disease (CD). One of the most common murine models for studying CD is IL-10-/- mouse. IL-10-/- mice when housed in conventional conditions and take contact with commensal microorganisms develop an acute enterocolitis mediated by a Th1 immune response. Even though, alterations in PCs function are related to CD, they had not been characterized yet in this mouse model. Here we show that in specific pathogen free conditions IL-10-/- mice have aberrant granules and a large number of immature PCs at the bottom of the crypt in the ileum of IL-10-/- mice before developing intestinal inflammation, along with a reduced expression of Indian Hedgehog. In addition, IL-10-/- Paneth cells presented a reduced expression of cryptidin-4, and a heterogeneous distribution of lysozyme+ granules. The alterations in the maturation of the PCs at the bottom of the crypt were not modified after the colonization by the conventional microbiota. On the other hand, depletion of microbiota altered the phenotype, but did not normalize PCs. Our results suggest that IL-10 could be necessary for the integrity of PCs. Moreover, our results help to explain why IL-10-/- mice develop enterocolitis in response to microorganisms.
dc.fechaingreso.objetodigital2024-04-02
dc.fuente.origenORCID
dc.identifier.doi10.1371/journal.pone.0221618
dc.identifier.urihttp://europepmc.org/abstract/med/31509557
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81062
dc.information.autorucFacultad de Ciencias Biológicas; Berkowitz Fiebich, Loni; S/I; 172010
dc.information.autorucFacultad de Ciencias Biológicas; Pardo Roa, Catalina; 0000-0002-5409-225X; 225251
dc.information.autorucEscuela de Medicina; Ramírez Rojas, Gigliola; S/I; 1001936
dc.information.autorucFacultad de Ciencias Biológicas; Vallejos Galvez, Omar Patricio; S/I; 215421
dc.information.autorucFacultad de Ciencias Biológicas; Sebastián Quijada, Valentina Pilar; S/I; 171925
dc.information.autorucEscuela de Medicina; Álvarez Lobos, Manuel; S/I; 6131
dc.information.autorucFacultad de Ciencias Biológicas; Bueno Ramírez, Susan; 0000-0002-7551-8088; 113541
dc.language.isoen
dc.nota.accesoContenido completo
dc.rightsacceso abierto
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleThe absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice.
dc.typeartículo
sipa.codpersvinculados172010
sipa.codpersvinculados225251
sipa.codpersvinculados1001936
sipa.codpersvinculados215421
sipa.codpersvinculados171925
sipa.codpersvinculados6131
sipa.codpersvinculados113541
sipa.trazabilidadORCID;2024-01-15
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