Expression of scavenger receptors in glial cells - Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid

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dc.contributor.authorAlarcon, R
dc.contributor.authorFuenzalida, C
dc.contributor.authorSantibanez, M
dc.contributor.authorvon Bernhardi, R
dc.date.accessioned2024-01-10T12:38:54Z
dc.date.available2024-01-10T12:38:54Z
dc.date.issued2005
dc.description.abstractAstrocytes and microglia associate to amyloid plaques, a pathological hallmark of Alzheimer disease. Microglia are activated by and can phagocytose beta-amyloid ( A beta). Scavenger receptors ( SRs) are among the receptors mediating the uptake of fibrillar A beta in vitro. However, little is known about the function of the astrocytes surrounding the plaques or the nature of their interaction with A beta. It is unknown whether glial cells bind to nonfibrillar A beta and if binding of astrocytes to A beta depends on the same Scavenger receptors described for microglia. We determined the binding of glia to A beta by an adhesion assay and evaluated the presence of scavenger receptors in glial cells by immunocytochemistry, immunohistochemistry of brain sections, and immunoblot. We found that astrocytes and microglia from neonatal rats adhered in a concentration-dependent manner to surfaces coated with fibrillar A beta or nonfibrillar A beta. Fucoidan and poly( I), known ligands for SR-type A, inhibited adhesion of microglia and astrocytes to A beta and also inhibited A beta phagocytosis. In contrast, a ligand for SR-type B like low density lipoprotein, did not compete glial adhesion to A beta. Microglia presented immunodetectable SR-BI, SR-AI/AII, RAGE, and SR-MARCO ( macrophage receptor with collagenous structure, a member of the SR-A family). Astrocytes presented SR-BI and SR-MARCO. To our knowledge, this is the first description of the presence of SR-MARCO in astrocytes. Our results indicate that both microglia and astrocytes adhere to fibrillar and nonfibrillar A beta. Adhesion was mediated by a fucoidan-sensitive receptor. We propose that SR-MARCO could be the Scavenger receptor responsible for the adhesion of astrocytes and microglia to A beta.
dc.fechaingreso.objetodigital2024-04-25
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M414686200
dc.identifier.eissn1083-351X
dc.identifier.pubmedidMEDLINE:15987691
dc.identifier.urihttps://doi.org/10.1074/jbc.M414686200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77116
dc.identifier.wosidWOS:000231362500053
dc.information.autorucMedicina;Von Bernhardi R;S/I;62523
dc.issue.numero34
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final30415
dc.pagina.inicio30406
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.revistaJOURNAL OF BIOLOGICAL CHEMISTRY
dc.rightsacceso abierto
dc.subjectLOW-DENSITY-LIPOPROTEIN
dc.subjectALZHEIMERS-DISEASE BRAIN
dc.subjectPLAQUE CORE PROTEIN
dc.subjectCLASS-B
dc.subjectNERVOUS-SYSTEM
dc.subjectSENILE PLAQUES
dc.subjectHOST-DEFENSE
dc.subjectIN-VITRO
dc.subjectINFLAMMATORY RESPONSE
dc.subjectCULTURED ASTROCYTES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExpression of scavenger receptors in glial cells - Comparing the adhesion of astrocytes and microglia from neonatal rats to surface-bound beta-amyloid
dc.typeartículo
dc.volumen280
sipa.codpersvinculados62523
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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