Expert elicitation of the diagnostic performance of two tests for Bacterial Kidney Disease (BKD) surveillance in Atlantic salmon (Salmo salar L.) broodstock in Chile

dc.article.number735274
dc.catalogadorpva
dc.contributor.authorVerdugo, C.
dc.contributor.authorZimin Veselkoff, Natalia
dc.contributor.authorGardner, I.A.
dc.contributor.authorMardones, F.O.
dc.date.accessioned2024-03-04T15:19:37Z
dc.date.available2024-03-04T15:19:37Z
dc.date.issued2020
dc.description.abstractThe objective of this study was to obtain diagnostic performance estimates for immunofluorescence antibody test (IFAT) and quantitative polymerase chain reaction (qPCR) tests, for Bacterial Kidney Disease (BKD) surveillance in farmed Atlantic salmon (Salmo salar.) broodstock without clinical signs compatible with the disease in Chile. To obtain diagnostic sensitivity (DSe) and specificity (DSp) estimates of those tests, an expert elicitation process was conducted using the trial roulette method during a workshop with 18 national experts on BKD diagnostics and control. A questionnaire was given to the experts to gather information on their previous experience with BKD, and 12 experts who received an introduction to the expert-elicitation process and graphical representation of statistical distributions. Experts were divided into three groups and requested to draw distributions of DSe and DSp parameters using an online tool. Then figures where projected and discussed among participants. After the discussion round, experts were requested to redraw their distributions (if considered necessary). The agreement between groups was assessed by calculating the overlapping areas between the three distributions for each parameter. Finally, a consensus distribution was generated. In general, groups had moderate to high agreement with overlapping areas between 53% to 93% for DSpIFAT, DSeqPCR, and DSpqPCR. Conversely, no agreement was reached for the DSeIFAT distribution. Specifically, median estimates of 89.5%, 90.5%, and 95.9%, were obtained for DSpIFAT, DSeqPCR, & DSpqPCR, respectively. In the case of DSeIFAT, the first group provided a median estimate of 28%, whereas groups 2 and 3 reached a consensus value of approximately 62%. This study provides information that is not available in the country, and will help to adjust current BKD control programs, which mainly rely on the use of qPCR assays.
dc.fechaingreso.objetodigital2024-02-26
dc.format.extent5 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.aquaculture.2020.735274
dc.identifier.eissn1873-5622
dc.identifier.issn0044-8486
dc.identifier.urihttps://doi.org/10.1016/j.aquaculture.2020.735274
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/82431
dc.information.autorucEscuela de Medicina Veterinaria; Mardones Loyola, Fernando Otoniel; 0000-0003-1228-5392; 1084534
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final5
dc.pagina.inicio1
dc.revistaAquaculture
dc.rightsacceso restringido
dc.subjectBacterial kidney disease
dc.subjectBKD
dc.subjectDiagnostic tests
dc.subjectSurveillance
dc.subjectExpert elicitation
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleExpert elicitation of the diagnostic performance of two tests for Bacterial Kidney Disease (BKD) surveillance in Atlantic salmon (Salmo salar L.) broodstock in Chile
dc.typeartículo
dc.volumen525
sipa.codpersvinculados1084534
sipa.trazabilidadORCID;2024-01-15
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