Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets

Simple item page
dc.catalogadorcrc
dc.contributor.authorPanzer, Jonathan J.
dc.contributor.authorRomero, Roberto
dc.contributor.authorGreenberg, Jonathan M.
dc.contributor.authorWinters, Andrew D.
dc.contributor.authorGalaz, Jose
dc.contributor.authorGomez-Lopez, Nardhy
dc.contributor.authorTheis, Kevin R.
dc.date.accessioned2023-03-27T15:11:23Z
dc.date.available2023-03-27T15:11:23Z
dc.date.issued2023
dc.date.updated2023-03-19T01:03:09Z
dc.description.abstractThe existence of a placental microbiota is debated. The human placenta has historically been considered sterile and microbial colonization was associated with adverse pregnancy outcomes. Yet, recent DNA sequencing investigations reported a microbiota in typical human term placentas. However, this detected microbiota could represent background DNA or delivery-associated contamination. Using fifteen publicly available 16S rRNA gene datasets, existing data were uniformly re-analyzed with DADA2 to maximize comparability. While Amplicon Sequence Variants (ASVs) identified as Lactobacillus, a typical vaginal bacterium, were highly abundant and prevalent across studies, this prevalence disappeared after applying likely  DNA contaminant removal to placentas from term cesarean deliveries. A six-study sub-analysis targeting the 16S rRNA gene V4 hypervariable region demonstrated that bacterial profiles of placental samples and technical controls share principal bacterial ASVs and that placental samples clustered primarily by study origin and mode of delivery. Contemporary DNA-based evidence does not support the existence of a placental microbiota. Importance Early-gestational microbial influences on human development are unclear. By applying DNA sequencing technologies to placental tissue, bacterial DNA signals were observed, leading some to conclude that a live bacterial placental microbiome exists in typical term pregnancy. However, the low-biomass nature of the proposed microbiome and high sensitivity of current DNA sequencing technologies indicate that the signal may alternatively derive from environmental or delivery-associated bacterial DNA contamination. Here we address these alternatives with a re-analysis of 16S rRNA gene sequencing data from 15 publicly available placental datasets. After identical DADA2 pipeline processing of the raw data, subanalyses were performed to control for mode of delivery and environmental DNA contamination. Both environment and mode of delivery profoundly influenced the bacterial DNA signal from term-delivered placentas. Aside from these contamination-associated signals, consistency was lacking across studies. Thus, placentas delivered at term are unlikely to be the original source of observed bacterial DNA signals.
dc.fechaingreso.objetodigital2023-03-27
dc.fuente.origenAutoarchivo
dc.identifier.citationPanzer, J.J., Romero, R., Greenberg, J.M. et al. Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets. BMC Microbiol 23, 76 (2023). https://doi.org/10.1186/s12866-023-02764-6
dc.identifier.doi10.1186/s12866-023-02764-6
dc.identifier.urihttps://doi.org/10.1186/s12866-023-02764-6
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66661
dc.information.autorucFacultad de Medicina;Galaz, José;0000-0002-8160-8581;1034327
dc.issue.numero76
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final20
dc.pagina.inicio1
dc.revistaBMC Microbiology
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject16S rRNA gene sequencing
dc.subjectLow microbial biomass sampling
dc.subjectMicrobiome
dc.subjectPlacenta
dc.subjectSterile womb
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleIs there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets
dc.typeartículo
dc.volumen23
sipa.codpersvinculados1034327
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12866_2023_Article_2764.pdf
Size:
3.24 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos de revistas