Brewer\'s spent grain extract modulates the immune, intercellular junctions and antioxidant gene expression disrupting the Piscirickettsia salmonis infection in rainbow trout intestinal epithelial RTgutGC cells

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dc.article.number110933
dc.catalogadorjlo
dc.contributor.authorCárcamo, Diego
dc.contributor.authorPérez, Tatiana
dc.contributor.authorSánchez, Fabiola
dc.contributor.authorOliver, Cristián
dc.contributor.authorGonzález, Luisbel
dc.contributor.authorRomero, Alex
dc.contributor.authorRavanal Espinosa, María Cristina
dc.date.accessioned2025-12-11T12:38:00Z
dc.date.available2025-12-11T12:38:00Z
dc.date.issued2026
dc.description.abstractSustainable mitigation of piscirickettsiosis, the leading infectious cause of mortality in Chilean salmon aquaculture and a key driver of antibiotic dependence, demands green alternatives that fortify the intestinal health of fish. Here we show that brewer's spent grain (BSG), an abundant lignocellulosic by-product, can be upgraded into a functional feed additive through a fully aqueous, green-chemistry workflow comprising enzymatic hydrolysis with cellulase/hemicellulase, microwave-vacuum drying, and ultrasound-assisted water extraction. The process yields a solvent-free extract [bagasse extract–PBS (BEP)1860 μg/mL gallic acid equivalents (GAE)] that preserves ≥90 % RTgutGC cell viability up to 35 μg/mL GAE. A 24 h pre-exposure of rainbow trout intestinal epithelial cells to 5–25 μg/mL GAE of BEP markedly enhanced the inflammatory response (il-1β and il-8 up-regulated up to 4.3-fold), strengthened barrier integrity (zo-1 up-regulated 3.1-fold), and activated antioxidant defenses (sod, gpx, gr elevated 2–5-fold). Importantly, pre-treatment with 25–100 μg/mL GAE of BEP curtailed Piscirickettsia salmonis induced cellular lysis by ∼45 % and reduced bacterial translocation across epithelial monolayers by ∼1 log CFU, with the strongest protection against the prevalent EM-90-like genotype. This is the first report demonstrating that an agro-industrial residue can be converted, within a biocircular and sustainable-pharmacy framework, into an immunonutraceutical capable of simultaneously promoting the optimal immune system activation, the modulation of intercellular junctions, and the oxidative stress in this intestinal epithelial cell line. The present study suggests the use of BEP as a potential additive capable of modulating innate immune responses and contributing to future mitigation of the impact of P. salmonis infection. However, further studies are necessary to test this important issue for the aquaculture industry in Chile.
dc.format.extent13 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.fsi.2025.110933
dc.identifier.issn1095-9947
dc.identifier.urihttps://doi.org/10.1016/j.fsi.2025.110933
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/107351
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal||Instituto para el Desarrollo Sustentable; Ravanal Espinosa, María Cristina; 0000-0003-2462-6270; 1400221
dc.language.isoen
dc.nota.accesocontenido parcial
dc.revistaFish and Shellfish Immunology
dc.rightsacceso restringido
dc.subjectBrewer's spent grain (BSG)
dc.subjectPiscirickettsia salmonis
dc.subjectFunctional additive
dc.subjectPolyphenols and RTgutGC
dc.subject.ddc570
dc.subject.ods14 Life below water
dc.subject.ods03 Good health and well-being
dc.subject.odspa14 Vida submarina
dc.subject.odspa03 Salud y bienestar
dc.titleBrewer\'s spent grain extract modulates the immune, intercellular junctions and antioxidant gene expression disrupting the Piscirickettsia salmonis infection in rainbow trout intestinal epithelial RTgutGC cells
dc.typeartículo
dc.volumen168
sipa.codpersvinculados1400221
sipa.trazabilidadORCID;2025-12-09
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