Differences in lung glutathione metabolism may account for rodent susceptibility in elastase-induced emphysema development

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dc.contributor.authorBorzone, Gisella R.
dc.contributor.authorLiberona, Leonel F.
dc.contributor.authorBustamante, Andrea P.
dc.contributor.authorSaez, Claudia G.
dc.contributor.authorOlmos, Pablo R.
dc.contributor.authorVecchiola, Andrea
dc.contributor.authorVillagran, Andrea
dc.contributor.authorSerrano, Carolina
dc.contributor.authorReyes, Tatiana P.
dc.date.accessioned2024-01-10T13:47:34Z
dc.date.available2024-01-10T13:47:34Z
dc.date.issued2009
dc.description.abstractBorzone GR, Liberona LF, Bustamante AP, Saez CG, Olmos PR, Vecchiola A, Villagran A, Serrano C, Reyes TP. Differences in lung glutathione metabolism may account for rodent susceptibility in elastase-induced emphysema development. Am J Physiol Regul Integr Comp Physiol 296: R1113-R1123, 2009. First published January 14, 2009; doi: 10.1152/ajpregu.90361.2008.-Syrian Golden hamsters develop more severe emphysema than Sprague-Dawley rats after intratracheal instillation of the same dose of elastase/body weight. Although species variations in antielastase defenses may largely explain these results, other variables, such as differences in lung antioxidants, cannot be overlooked since oxidative stress modulates antiprotease activity. We propose that elastase instillation might affect lung glutathione (GSH) metabolism differently in these species. Our aim was to study in hamsters and rats, lung glutathione metabolism at different times, from the stage of diffuse alveolar damage to advanced emphysema. We measured total and oxidized glutathione content as well as activity and expression of enzymes related to GSH synthesis and redox cycling: gamma-glutamylcysteine synthetase, glutathione peroxidase, and glutathione reductase. Whereas rats showed no significant changes in these measurements, hamsters showed significant derangement in GSH metabolism early after elastase instillation: 25% fall in total GSH (P < 0.05) with no increase in oxidized glutathione associated with reduced enzyme activities 24 h after elastase [60% for gamma-glutamylcysteine synthetase (P < 0.01), 30% for glutathione peroxidase (P < 0.01), and 75% for glutathione reductase (P < 0.001)]. GSH homeostasis was restored at the end of the first week, involving transient increased expression of these enzymes. We conclude that elastase induces significant alterations in GSH metabolism of hamster lungs and no overall change in rat lungs. Although differences in disease severity may account for our findings, the hamster becomes vulnerable to functional inhibition of alpha(1)-antitrypsin by oxidants and thus, even more susceptible to injury than it would be, considering only its low alpha(1)-antitrypsin level.
dc.description.funderNational Fund for Scientific and Technological Development (FONDECYT)
dc.fechaingreso.objetodigital2024-05-06
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1152/ajpregu.90361.2008
dc.identifier.eissn1522-1490
dc.identifier.issn0363-6119
dc.identifier.pubmedidMEDLINE:19144757
dc.identifier.urihttps://doi.org/10.1152/ajpregu.90361.2008
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79284
dc.identifier.wosidWOS:000264565100031
dc.information.autorucMedicina;Borzone GR ;S/I;99892
dc.information.autorucMedicina;Bustamante AP;S/I;5311
dc.information.autorucMedicina;Liberona LF;S/I;1000482
dc.information.autorucMedicina;Olmos PR;S/I;98949
dc.information.autorucMedicina;Reyes TP;S/I;4013
dc.information.autorucMedicina;Saez CG ;S/I;102375
dc.information.autorucMedicina;Vecchiola A ;S/I;123319
dc.information.autorucMedicina;Villagran A;S/I;1004414
dc.issue.numero4
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherAMER PHYSIOLOGICAL SOC
dc.revistaAMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
dc.rightsregistro bibliográfico
dc.subjectdiffuse alveolar damage
dc.subjectgamma-glutamyl-cysteine synthetase
dc.subjectglutathione peroxidase
dc.subjectglutathione reductase
dc.subjectlung susceptibility to elastase
dc.subjectpulmonary emphysema
dc.subjectGAMMA-GLUTAMYLCYSTEINE SYNTHETASE
dc.subjectINDUCED PULMONARY-EMPHYSEMA
dc.subjectOXIDATIVE STRESS
dc.subjectCIGARETTE-SMOKE
dc.subjectRAT LUNG
dc.subjectINHIBITORY CAPACITY
dc.subjectGENE-EXPRESSION
dc.subjectENZYME-ACTIVITIES
dc.subjectPEROXIDASE
dc.subjectREDUCTASE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferences in lung glutathione metabolism may account for rodent susceptibility in elastase-induced emphysema development
dc.typeartículo
dc.volumen296
sipa.codpersvinculados99892
sipa.codpersvinculados5311
sipa.codpersvinculados1000482
sipa.codpersvinculados98949
sipa.codpersvinculados4013
sipa.codpersvinculados102375
sipa.codpersvinculados123319
sipa.codpersvinculados1004414
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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