Lipopolysaccharide-induced carotid body inflammation in cats: functional manifestations, histopathology and involvement of tumour necrosis factor-alpha

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dc.contributor.authorFernandez, Ricardo
dc.contributor.authorGonzalez, Sergio
dc.contributor.authorRey, Sergio
dc.contributor.authorCortes, Paula P.
dc.contributor.authorMaisey, Kevin R.
dc.contributor.authorReyes, Edison Pablo
dc.contributor.authorLarrain, Carolina
dc.contributor.authorZapata, Patricio
dc.date.accessioned2024-01-10T13:50:01Z
dc.date.available2024-01-10T13:50:01Z
dc.date.issued2008
dc.description.abstractIn the absence of information on functional manifestations of carotid body (CB) inflammation, we studied an experimental model in which lipopolysaccharide (LPS) administration to pentobarbitone-anaesthetized cats was performed by topical application upon the CB surface or by intravenous infusion (endotoxaemia). The latter caused: (i) disorganization of CB glomoids, increased connective tissue, and rapid recruitment of polymorphonuclear cells into the vascular bed and parenchyma within 4 h; (ii) increased respiratory frequency and diminished ventilatory chemoreflex responses to brief hypoxia (breathing 100% N-2 for 10 s) and diminished ventilatory chemosensory drive (assessed by 100% O-2 tests) during normoxia and hypoxia; (iii) tachycardia, increased haematocrit and systemic hypotension in response to LPS I.V.; and (iv) increased basal frequency of carotid chemosensory discharges during normoxia, but no change in maximal chemoreceptor responses to brief hypoxic exposures. Lipopolysaccharide-induced tachypnoea was prevented by prior bilateral carotid neurotomy. Apoptosis was not observed in CBs from cats subjected to endotoxaemia. Searching for pro-inflammatory mediators, tumour necrosis factor-alpha (TNF-alpha) was localized by immunohistochemistry in glomus and endothelial cells; reverse transcriptase-polymerase chain reaction revealed that the CB expresses the mRNAs for both type-1 (TNF-R1) and type-2 TNF-alpha receptors (TNF-R2); Western blot confirmed a band of the size expected for TNF-R1; and histochemistry showed the presence of TNF-R1 in glomus cells and of TNF-R2 in endothelial cells. Experiments in vitro showed that the frequency of carotid nerve discharges recorded from CBs perfused and superfused under normoxic conditions was not significantly modified by TNF-alpha, but that the enhanced frequency of chemosensory discharges recorded along responses to hypoxic stimulation was transiently diminished in a dose-dependent manner by TNF-alpha injections. The results suggest that the CB may operate as a sensor for immune signals, that the CB exhibits histological features of acute inflammation induced by LPS, that TNF-alpha may participate in LPS-induced changes in chemosensory activity and that some pathophysiological reactions to high levels of LPS in the bloodstream may originate from changes in CB function.
dc.fechaingreso.objetodigital2024-05-10
dc.format.extent16 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1113/expphysiol.2008.041152
dc.identifier.eissn1469-445X
dc.identifier.issn0958-0670
dc.identifier.pubmedidMEDLINE:18562477
dc.identifier.urihttps://doi.org/10.1113/expphysiol.2008.041152
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79499
dc.identifier.wosidWOS:000256722300012
dc.information.autorucMedicina;González S;S/I;99856
dc.information.autorucMedicina;Rey S;S/I;1083
dc.information.autorucMedicina;Zapata P;S/I;98016
dc.issue.numero7
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final907
dc.pagina.inicio892
dc.publisherWILEY-BLACKWELL
dc.revistaEXPERIMENTAL PHYSIOLOGY
dc.rightsregistro bibliográfico
dc.subjectDORSAL VAGAL COMPLEX
dc.subjectC-FOS EXPRESSION
dc.subjectRECEPTOR-TYPE-I
dc.subjectSUBDIAPHRAGMATIC VAGOTOMY
dc.subjectENDOTOXIN INFUSION
dc.subjectESCHERICHIA-COLI
dc.subjectGLOMUS CELLS
dc.subjectVAGUS NERVE
dc.subjectINTERLEUKIN-1
dc.subjectRAT
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLipopolysaccharide-induced carotid body inflammation in cats: functional manifestations, histopathology and involvement of tumour necrosis factor-alpha
dc.typeartículo
dc.volumen93
sipa.codpersvinculados99856
sipa.codpersvinculados1083
sipa.codpersvinculados98016
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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