Chemogenetic inhibition of NTS astrocytes normalizes cardiac autonomic control and ameliorate hypertension during chronic intermittent hypoxia

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dc.article.number57
dc.catalogadoraba
dc.contributor.authorPereyra Florián, Katherin Vanessa
dc.contributor.authorLas Heras, Alexandra
dc.contributor.authorToledo, Camilo
dc.contributor.authorDíaz-Jara, Esteban
dc.contributor.authorIturriaga, Rodrigo
dc.contributor.authorRío Troncoso, Rodrigo Andre del
dc.date.accessioned2023-11-14T15:32:23Z
dc.date.available2023-11-14T15:32:23Z
dc.date.issued2023
dc.date.updated2023-11-12T01:03:08Z
dc.description.abstractAbstract Background Obstructive sleep apnea (OSA) is characterized by recurrent episodes of chronic intermittent hypoxia (CIH), which has been linked to the development of sympathoexcitation and hypertension. Furthermore, it has been shown that CIH induced inflammation and neuronal hyperactivation in the nucleus of the solitary tract (NTS), a key brainstem region involved in sympathetic and cardiovascular regulation. Since several studies have proposed that NTS astrocytes may mediate neuroinflammation, we aimed to determine the potential contribution of NTS-astrocytes on the pathogenesis of CIH-induced hypertension. Results Twenty-one days of CIH induced autonomic imbalance and hypertension in rats. Notably, acute chemogenetic inhibition (CNO) of medullary NTS astrocytes using Designer Receptors Exclusively Activated by Designers Drugs (DREADD) restored normal cardiac variability (LF/HF: 1.1 ± 0.2 vs. 2.4 ± 0.2 vs. 1.4 ± 0.3, Sham vs. CIH vs. CIH + CNO, respectively) and markedly reduced arterial blood pressure in rats exposed to CIH (MABP: 82.7 ± 1.2 vs. 104.8 ± 4.4 vs. 89.6 ± 0.9 mmHg, Sham vs. CIH vs. CIH + CNO, respectively). In addition, the potentiated sympathoexcitation elicit by acute hypoxic chemoreflex activation in rats exposed to CIH was also completely abolished by chemogenetic inhibition of NTS astrocytes using DREADDs. Conclusion Our results support a role for NTS astrocytes in the maintenance of heightened sympathetic drive and hypertension during chronic exposure to intermittent hypoxia mimicking OSA.
dc.fechaingreso.objetodigital2023-11-14
dc.fuente.origenBiomed Central
dc.identifier.doi10.1186/s40659-023-00463-0
dc.identifier.issn0717-6287
dc.identifier.urihttps://doi.org/10.1186/s40659-023-00463-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75303
dc.information.autorucFacultad de Ciencias Biológicas; Río Troncoso, Rodrigo Andre del; S/I; 126608
dc.information.autorucFacultad de Ciencias Biológicas; Pereyra Florián, Katherin Vanessa; S/I; 1027823
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final8
dc.pagina.inicio1
dc.revistaBiological Research
dc.rightsacceso abierto
dc.subjectObstructive sleep apnea
dc.subjectIntermittent hypoxia
dc.subjectAstrocytes
dc.subjectNucleus of the solitary tract
dc.subjectReactive astrocytes
dc.subjectNeuroinflammation
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleChemogenetic inhibition of NTS astrocytes normalizes cardiac autonomic control and ameliorate hypertension during chronic intermittent hypoxia
dc.typeartículo
dc.volumen56
sipa.codpersvinculados126608
sipa.codpersvinculados1027823
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