Cultured autologous bone marrow stem cells inhibit bony fusion in a rabbit model of posterolateral lumbar fusion with autologous bone graft

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dc.contributor.authorUrrutia, Julio
dc.contributor.authorMery, Pablo
dc.contributor.authorMartinez, Rafael
dc.contributor.authorPizarro, Felipe
dc.contributor.authorApablaza, Daniel
dc.contributor.authorMardones, Rodrigo
dc.date.accessioned2024-01-10T12:04:48Z
dc.date.available2024-01-10T12:04:48Z
dc.date.issued2010
dc.description.abstractMesenchymal stem cells (MSCs) have been isolated from various tissues and expanded in culture. MSCs add osteogenic potential to ceramic scaffolds when used together. A spinal fusion rabbit model was used to evaluate whether a pellet of cultured, autologous bone marrow MSCs (BMSCs) with osteogenic differentiation could increase the fusion rate when co-grafted with an autologus bone graft compared to autograft alone. Thirty rabbits were randomly assigned to two groups. Group I received bone autograft alone and Group 2 received bone autograft plus a pellet of cultured and differentiated BMSCs. Group 2 rabbits had a bone marrow puncture, after which the BMSC were cultured and osteoblastic differentiation was induced. BMSC cultures were obtained from 12 of 15 rabbits. The 27 rabbits underwent a bilateral, L4-L5 intertransverse fusion with an autograft and in Group 2 rabbits a pellet of differentiated BMSCs was added to the autograft. In Group 1, the fusion rate was 53% (8 of 15 rabbits) and ill Group 2 the fusion rate was 0% (p < 0.05). Adding differentiated BMSCs in a pellet without a scaffold not only failed to increase fusion rate, but completely inhibited bony growth. (C) 2009 Elsevier Ltd. All rights reserved.
dc.fechaingreso.objetodigital27-03-2024
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jocn.2009.06.024
dc.identifier.eissn1532-2653
dc.identifier.issn0967-5868
dc.identifier.pubmedidMEDLINE:20171892
dc.identifier.urihttps://doi.org/10.1016/j.jocn.2009.06.024
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75883
dc.identifier.wosidWOS:000276014600016
dc.information.autorucMedicina;Apablaza D;S/I;129229
dc.information.autorucMedicina;Martínez R;S/I;13386
dc.information.autorucMedicina;Mery P;S/I;14013
dc.information.autorucMedicina;Pizarro F;S/I;135686
dc.information.autorucMedicina;Urrutia J;S/I;69910
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final485
dc.pagina.inicio481
dc.publisherELSEVIER SCI LTD
dc.revistaJOURNAL OF CLINICAL NEUROSCIENCE
dc.rightsacceso restringido
dc.subjectAnimal model
dc.subjectLumbar spinal fusion
dc.subjectStem cells
dc.subjectHUMAN ADIPOSE-TISSUE
dc.subjectSPINAL-FUSION
dc.subjectIN-VIVO
dc.subjectPOROUS HYDROXYAPATITE
dc.subjectGROWTH-FACTORS
dc.subjectDIFFERENTIATION
dc.subjectCOMPOSITES
dc.subjectCERAMICS
dc.subjectIMPLANTS
dc.subjectTHERAPY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCultured autologous bone marrow stem cells inhibit bony fusion in a rabbit model of posterolateral lumbar fusion with autologous bone graft
dc.typeartículo
dc.volumen17
sipa.codpersvinculados129229
sipa.codpersvinculados13386
sipa.codpersvinculados14013
sipa.codpersvinculados135686
sipa.codpersvinculados69910
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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