Extracellular Vesicles derived from Apis mellifera Royal Jelly promote wound healing by modulating inflammation and cellular responses

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dc.catalogadoraba
dc.contributor.authorÁlvarez, Simón
dc.contributor.authorContreras Kallens, Pamina
dc.contributor.authorAguayo Paul, Sebastián
dc.contributor.authorRamírez, Orlando
dc.contributor.authorVallejos, Catalina
dc.contributor.authorRuiz, Jorge
dc.contributor.authorCarrasco Gallardo, Eva
dc.contributor.authorTroncoso Vera, Stephanie
dc.contributor.authorMorales, Bernardo
dc.contributor.authorSchuh, Christina
dc.date.accessioned2023-08-11T22:05:39Z
dc.date.available2023-08-11T22:05:39Z
dc.date.issued2022
dc.description.abstractApis mellifera Royal Jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. Several active compounds have been identified, however, the mechanisms of action still remain widely unknown. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs) and in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing associated effects. Molecular analysis of RJEVs verified the presence of important conserved exosomal markers such as CD63 and syntenin, as well as cargo molecules MRJP1, defensin-1 and jellein-3. RJEV internalization analysis demonstrated the involvement of membrane fusion as well as macropinocytosis or clathrin-dependent endocytosis into mammalian cells. Furthermore, RJEVs have demonstrated to modulate MSCs differentiation and secretome, as well as decrease LPS-induced inflammation in RAW 264.7 macrophages by blocking the MAPK pathway. In vivo studies confirmed anti-bacterial effects of RJEVs, and demonstrated an acceleration of wound healing in a splinted mouse model. Summarizing, this study suggests that RJEVs of potentially exosomal origin play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing.
dc.fechaingreso.objetodigital2023-07-06
dc.format.extent24 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1101/2022.07.21.501009
dc.identifier.urihttps://doi.org/10.1101/2022.07.21.501009
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74391
dc.information.autorucEscuela de Medicina; Aguayo Paul, Sebastián; 0000-0003-0900-1993; 1062336
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final24
dc.pagina.inicio1
dc.revistaBioRxiv
dc.rightsacceso restringido
dc.subjectInterkingdom Communicationes_ES
dc.subjectAnti-inflammatory Extracellular Vesicleses_ES
dc.subjectGland-derived Extracellular Vesicleses_ES
dc.subjectDefensin-1;es_ES
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleExtracellular Vesicles derived from Apis mellifera Royal Jelly promote wound healing by modulating inflammation and cellular responseses_ES
dc.typepreprint
sipa.codpersvinculados1062336
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