In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents

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dc.contributor.authorGuzman, Leda
dc.contributor.authorVillalon, Katherine
dc.contributor.authorJose Marchant, Maria
dc.contributor.authorElena Tarnok, Maria
dc.contributor.authorCardenas, Pilar
dc.contributor.authorAquea, Gisela
dc.contributor.authorAcevedo, Waldo
dc.contributor.authorPadilla, Leandro
dc.contributor.authorBernal, Giuliano
dc.contributor.authorMolinari, Aurora
dc.contributor.authorCorvalan, Alejandro
dc.date.accessioned2024-01-10T13:14:06Z
dc.date.available2024-01-10T13:14:06Z
dc.date.issued2020
dc.description.abstractThe cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC50 values: 7.1 mu M (QS-21) and 13.6 mu M (QA) on SNU1 cells; 7.4 mu M (QS-21) and 67 mu M (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (G(bin)) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer.
dc.description.funderDireccion de Investigacion de la Vicerrectoria de Investigacion y Estudios Avanzados
dc.description.funderPontificia Universidad Catolica de Valparaiso, Chile
dc.description.funderCONICYT-FONDAP
dc.description.funderCONICY
dc.description.funderDESERT KING Chile
dc.fechaingreso.objetodigital2024-05-09
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41598-020-67442-3
dc.identifier.issn2045-2322
dc.identifier.pubmedidMEDLINE:32601436
dc.identifier.urihttps://doi.org/10.1038/s41598-020-67442-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78373
dc.identifier.wosidWOS:000548359400007
dc.information.autorucArquitectura;Corvalan Rodriguez Alejandro;S/I;63885
dc.information.autorucCiencias Biológicas;Guzman Maluenda Leda;S/I;104061
dc.information.autorucCiencias Biológicas;Padilla Iglesias Leandro Mauri;S/I;93966
dc.issue.numero1
dc.language.isoen
dc.nota.accesoSin adjunto
dc.publisherNATURE RESEARCH
dc.revistaSCIENTIFIC REPORTS
dc.rightsregistro bibliográfico
dc.subjectCELL-DEATH
dc.subjectAPOPTOSIS
dc.subjectSAPONINS
dc.subjectGROWTH
dc.subjectFAS
dc.subjectSTAUROSPORINE
dc.subjectINDUCTION
dc.subjectLIFE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIn vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
dc.typeartículo
dc.volumen10
sipa.codpersvinculados63885
sipa.codpersvinculados104061
sipa.codpersvinculados93966
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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