Prednisolone has a positive effect on the kidney but not on the liver of brain dead rats: a potencial role in complement activation

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dc.contributor.authorRebolledo Acevedo, Rolando Arturo
dc.contributor.authorLiu, Bo
dc.contributor.authorAkhtar, Mohammed Z.
dc.contributor.authorOttens, Petra J.
dc.contributor.authorZhang, Jian-Ning
dc.contributor.authorPloeg, Rutger J.
dc.contributor.authorLeuvenink, Henri G. D.
dc.date.accessioned2023-01-13T19:14:42Z
dc.date.available2023-01-13T19:14:42Z
dc.date.issued2014
dc.description.abstractBackground: Contradictory evidence has been published on the effects of steroid treatments on the outcomes of kidney and liver transplantation from brain dead (BD) donors. Our study aimed to evaluate this disparity by investigating the effect of prednisolone administration on BD rats. Methods: BD induction was performed in ventilated rats by inflating a Fogarty catheter placed in the epidural space. Prednisolone (22.5 mg/kg) was administered 30 min prior to BD induction. After four hours of determination of BD: serum, kidney and liver tissues samples were collected and stored. RT-qPCR, routine biochemistry and immunohistochemistry were performed. Results: Prednisolone treatment reduced circulating IL-6 and creatinine plasma levels but not serum AST, ALT or LDH. Polymorphonuclear influx assessed by histology, and inflammatory gene expression were reduced in the kidney and liver. However, complement component 3 (C3) expression was decreased in kidney but not in liver. Gene expression of HSP-70, a cytoprotective protein, was down-regulated in the liver after treatment. Conclusions: This study shows that prednisolone decreases inflammation and improves renal function, whilst not reducing liver injury. The persistence of complement activation and the negative effect on protective cellular mechanisms in the liver may explain the disparity between the effects of prednisolone on the kidney and liver of BD rats. The difference in the molecular and cellular responses to prednisolone administration may explain the contradictory evidence of the effects of prednisolone on different organ types from brain dead organ donors.
dc.fuente.origenSIPA
dc.identifier.doi10.1186/1479-5876-12-111
dc.identifier.issn1479-5876
dc.identifier.urihttps://doi.org/10.1186/1479-5876-12-111
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/66303
dc.information.autorucEscuela de medicina ; Rebolledo Acevedo, Rolando Arturo ; 0000-0003-3501-4042 ; 127520
dc.issue.numero111
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final10
dc.pagina.inicio1
dc.revistaJournal of Translational Medicinees_ES
dc.rightsacceso abierto
dc.subjectBrain deathes_ES
dc.subjectOrgan donationes_ES
dc.subjectSteroidses_ES
dc.subjectPrednisolonees_ES
dc.subjectKidney transplantationes_ES
dc.subjectLiver transplantationes_ES
dc.titlePrednisolone has a positive effect on the kidney but not on the liver of brain dead rats: a potencial role in complement activationes_ES
dc.typeartículo
dc.volumen12
sipa.codpersvinculados127520
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