Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line

dc.contributor.authorBuchegger, Kurt.
dc.contributor.authorIli, Carmen.
dc.contributor.authorRiquelme, Ismael.
dc.contributor.authorLetelier, Pablo.
dc.contributor.authorCorvalán R., Alejandro
dc.contributor.authorBrebi, Priscilla.
dc.contributor.authorHuang, Tim H.
dc.contributor.authorRoa Strauch, Juan Carlos Enrique
dc.date.accessioned2019-10-17T18:22:37Z
dc.date.available2019-10-17T18:22:37Z
dc.date.issued2016
dc.date.updated2019-10-14T19:10:52Z
dc.description.abstractAbstract Background Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. Methods The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5′-Aza-2′-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. Results In the cancer methylome system (CMS) (web-based system for visualizing and analyzing genome-wide methylation data of human cancers), the CpG island region of RPRM (1.1 kb) was hypermethylated in breast cancer compared to normal breast tissue; more interesting still was that ERα(+) tumors showed higher methylation intensity than ERα(−). Downregulation of RPRM mRNA by methylation was confirmed in MDA-MB-231 and BT-20 cell lines. In addition, overexpression of RPRM in MDA-MB-231 cells resulted in decreased rates of cell migration, wound healing and invasion in vitro. However, RPRM overexpression did not alter cell viability, phosphatidylserine (PS) translocation or G2/M cell cycle transition. Conclusion Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.
dc.fuente.origenBiomed Central
dc.identifier.citationBiological Research. 2016 Jan 22;49(1):5
dc.identifier.doi10.1186/s40659-016-0066-7
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26842
dc.issue.numeroNo.5
dc.language.isoen
dc.pagina.final10
dc.pagina.inicio1
dc.revistaBiological Researches_ES
dc.rightsacceso abierto
dc.rights.holderBuchegger et al.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherCáncer de mamaes_ES
dc.subject.otherCáncer de mama - Terapeaes_ES
dc.subject.otherCélulas cancerosas - Análisises_ES
dc.titleReprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell linees_ES
dc.typeartículo
dc.volumenVol.49
sipa.codpersvinculados84743
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