NO production and eNOS phosphorylation induced by epinephrine through the activation of β-adrenoceptors

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dc.contributor.authorFigueroa, Xavier
dc.contributor.authorCortés Mora, Víctor Antonio
dc.contributor.authorHuidobro-Toro, Juan Pablo.
dc.contributor.authorPoblete, Inés
dc.contributor.authorFernández Acevedo, Ricardo Hernán
dc.contributor.authorPedemonte Trewhela, Juan Cristóbal
dc.date.accessioned2017-04-25T19:24:43Z
dc.date.available2017-04-25T19:24:43Z
dc.date.issued2009
dc.description.abstractEpinephrine plays a key role in the control of vasomotor tone; however, the participation of the NO/cGMP pathway in response to β-adrenoceptor activation remains controversial. To evaluate the involvement of the endothelium in the vascular response to epinephrine, we assessed NO production, endothelial NO synthase phosphorylation, and tissue accumulation of cGMP in the perfused arterial mesenteric bed of rat. Epinephrine elicited a concentration-dependent increase in NO (EC50 of 45.7 pM), which was coupled to cGMP tissue accumulation. Both NO and cGMP production were blocked by either endothelium removal (saponin) or NO synthase inhibition (Nω-nitro-l-arginine). Blockade of β1- and β2-adrenoceptors with 1 μM propranolol or β3-adrenoceptor with 10 nM SR 59230A displaced rightward the concentration-NO production curve evoked by epinephrine. Selective stimulation of β1-, β2-, or β3-adrenoceptors also resulted in NO and cGMP production. Propranolol (1 μM) inhibited the rise in NO induced by isoproterenol or the β2-adrenoceptor agonists salbutamol, terbutaline, or fenoterol. Likewise, 10 nM SR 59230A reduced the effects of the β3-adrenoceptor agonists BRL 37344, CGP 12177, SR 595611A, or pindolol. The NO production induced by epinephrine and BRL 37344 was associated with the activation of the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of eNOS in serine 1177. In addition, in anaesthetized rats, bolus administration of isoproterenol, salbutamol, or BRL 37344 produced NO-dependent reductions in systolic blood pressure. These findings indicate that β1-, β2-, and β3-adrenoceptors are coupled to the NO/cGMP pathway, highlighting the role of the endothelium in the vasomotor action elicited by epinephrine and related β-adrenoceptor agonists.
dc.identifier.doi10.1152/ajpheart.00023.2009
dc.identifier.issn0363-6135
dc.identifier.urihttps://doi.org/10.1152/ajpheart.00023.2009
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/20442
dc.information.autorucFacultad de ciencias biológicas ; Figueroa, Xavier ; 0000-0001-9656-9100 ; 1437
dc.information.autorucFacultad de ciencias biológicas ; Fernández Acevedo, Ricardo Hernán ; 0000-0003-4493-9362 ; 122105
dc.information.autorucEscuela de medicina ; Pedemonte T., Juan Cristóbal ; S/I ; 119581
dc.information.autorucEscuela de medicina ; Cortés Mora, Víctor Antonio ; 0000-0002-1658-0965 ; 7576
dc.issue.numeroNo. 1
dc.language.isoen
dc.nota.accesoContenido completo
dc.relation.isformatofAmerican Journal of Physiology-Heart and Circulatory Physiology Vol. 297, no. 1 (2009), p. [H134]-H143
dc.revistaAmerican Journal of Physiology-Heart and Circulatory Physiologyes_ES
dc.rightsacceso abierto
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.otherReceptores adrenérgicoses_ES
dc.subject.otherEndotelio vasculares_ES
dc.subject.otherEpinefrinaes_ES
dc.titleNO production and eNOS phosphorylation induced by epinephrine through the activation of β-adrenoceptorses_ES
dc.typeartículo
dc.volumenVol. 297
sipa.codpersvinculados1437
sipa.codpersvinculados7576
sipa.codpersvinculados98862
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