Browsing by Author "Pereyra Florián, Katherin Vanessa"
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- ItemChemogenetic inhibition of NTS astrocytes normalizes cardiac autonomic control and ameliorate hypertension during chronic intermittent hypoxia(2023) Pereyra Florián, Katherin Vanessa; Las Heras, Alexandra; Toledo, Camilo; Díaz-Jara, Esteban; Iturriaga, Rodrigo; Río Troncoso, Rodrigo Andre delAbstract Background Obstructive sleep apnea (OSA) is characterized by recurrent episodes of chronic intermittent hypoxia (CIH), which has been linked to the development of sympathoexcitation and hypertension. Furthermore, it has been shown that CIH induced inflammation and neuronal hyperactivation in the nucleus of the solitary tract (NTS), a key brainstem region involved in sympathetic and cardiovascular regulation. Since several studies have proposed that NTS astrocytes may mediate neuroinflammation, we aimed to determine the potential contribution of NTS-astrocytes on the pathogenesis of CIH-induced hypertension. Results Twenty-one days of CIH induced autonomic imbalance and hypertension in rats. Notably, acute chemogenetic inhibition (CNO) of medullary NTS astrocytes using Designer Receptors Exclusively Activated by Designers Drugs (DREADD) restored normal cardiac variability (LF/HF: 1.1 ± 0.2 vs. 2.4 ± 0.2 vs. 1.4 ± 0.3, Sham vs. CIH vs. CIH + CNO, respectively) and markedly reduced arterial blood pressure in rats exposed to CIH (MABP: 82.7 ± 1.2 vs. 104.8 ± 4.4 vs. 89.6 ± 0.9 mmHg, Sham vs. CIH vs. CIH + CNO, respectively). In addition, the potentiated sympathoexcitation elicit by acute hypoxic chemoreflex activation in rats exposed to CIH was also completely abolished by chemogenetic inhibition of NTS astrocytes using DREADDs. Conclusion Our results support a role for NTS astrocytes in the maintenance of heightened sympathetic drive and hypertension during chronic exposure to intermittent hypoxia mimicking OSA.
- ItemCorrection to : Effects of enriched-potassium diet on cardiorespiratory outcomes in experimental non-ischemic chronic heart failure(2022) Schwarz Flores, Karla Gabriele; Pereyra Florián, Katherin Vanessa; Toledo, Camilo; Andrade Andrade, David Cristóbal; Díaz, Hugo S.; Díaz Jara, Esteban; Ortolani, Domiziana; Ríos Gallardo, Angélica; Arias, Paulina; Las Heras, AlexandraAn amendment to this paper has been published and can be accessed via the original article.
- ItemDietary supplementation of a sulforaphane-enriched broccoli extract protects the heart from acute cardiac stress(2020) Pereyra Florián, Katherin Vanessa; Andrade Andrade, David Cristóbal; Toledo, C.; Schwarz Flores, Karla Gabriele; Uribe-Ojeda, A.; Ríos-Gallardo, A. P.; Quintanilla, Rodrigo A.; Contreras E., Samuel; Mahn, A.; Río Troncoso, Rodrigo Andre del
- ItemEffects of enriched-potassium diet on cardiorespiratory outcomes in experimental non-ischemic chronic heart failure(2021) Schwarz Flores, Karla Gabriele; Pereyra Florián, Katherin Vanessa; Toledo, Camilo; Andrade Andrade, David Cristóbal; Díaz, Hugo S.; Díaz Jara, Esteban; Ortiz Cisternas, Fernando Andrés; Inestrosa Cantín, Nibaldo; Vio Lagos, Carlos P.; Río Troncoso, Rodrigo Andre delChronic heart failure (CHF) is a global health problem. Increased sympathetic outfow, cardiac arrhythmogenesis and irregular breathing patterns have all been associated with poor outcomes in CHF. Several studies showed that activation of the renin-angiotensin system (RAS) play a key role in CHF pathophysiology. Interestingly, potassium (K+) supplemented diets showed promising results in normalizing RAS axis and autonomic dysfunction in vascular diseases, lowering cardiovascular risk. Whether subtle increases in dietary K+ consumption may exert similar efects in CHF has not been previously tested. Accordingly, we aimed to evaluate the efects of dietary K+ supplementation on cardiorespiratory alterations in rats with CHF. Methods: Adult male Sprague–Dawley rats underwent volume overload to induce non-ischemic CHF. Animals were randomly allocated to normal chow diet (CHF group) or supplemented K+ diet (CHF+K+ group) for 6 weeks. Cardiac arrhythmogenesis, sympathetic outfow, barorefex sensitivity, breathing disorders, chemorefex function, respiratory–cardiovascular coupling and cardiac function were evaluated. Results: Compared to normal chow diet, K+ supplemented diet in CHF signifcantly reduced arrhythmia incidence (67.8±15.1 vs. 31.0±3.7 events/hour, CHF vs. CHF+K+), decreased cardiac sympathetic tone (ΔHR to propranolol: −97.4±9.4 vs. −60.8±8.3 bpm, CHF vs. CHF+K+), restored barorefex function and attenuated irregular breathing patterns. Additionally, supplementation of the diet with K+ restores normal central respiratory chemorefex drive and abrogates pathological cardio-respiratory coupling in CHF rats being the outcome an improved cardiac function. Conclusion: Our fndings support that dietary K+ supplementation in non-ischemic CHF alleviate cardiorespiratory dysfunction.