Colecciones Institucionales

Permanent URI for this community

Now showing 1 - 1 of 1

Isobaric labeling and tandem mass spectrometry: A novel approach for profiling and quantifying proteins differentially expressed in amniotic fluid in preterm labor with and without intra-amniotic infection/inflammation
(TAYLOR & FRANCIS LTD, 2010) Romero, Roberto; Kusanovic, Juan Pedro; Gotsch, Francesca; Erez, Offer; Vaisbuch, Edi; Mazaki Tovi, Shali; Moser, Allan; Tam, Sunny; Leszyk, John; Master, Stephen R.; Juhasz, Peter; Pacora, Percy; Ogge, Giovanna; Gomez, Ricardo; Yoon, Bo H.; Yeo, Lami; Hassan, Sonia S.; Rogers, Wade T.
Methods. A cross-sectional study was designed and included AF samples from patients with spontaneous PTL and intact membranes in the following groups: (1) patients without IAI who delivered at term (n = 26); (2) patients who delivered preterm without IAI (n = 25); and (3) patients with IAI (n = 24). Proteomic profiling of AF samples was performed using a workflow involving tryptic digestion, iTRAQ labeling and multiplexing, strong cation exchange fractionation, and liquid chromatography tandem mass spectrometry. Twenty-five separate 4-plex samples were prepared and analyzed.