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Browsing Colecciones Institucionales by browse.metadata.categoriaods "03 Salud y Bienestar"

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    Purinergic signalling modulates B cell activation
    (2024) Alamo Rollandi, Martina Roxana; Yuseff Sepúlveda, María Isabel; Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas
    B lymphocytes are cells of the adaptive immune system that produce specific antibodies after activating their BCR by antigen recognition, forming an immunological synapse that facilitates synapse that facilitates antigen retrieval, processing and presentation.While B-lymphocytes are known to respond to various microenvironmental signals, the full extent of their activation remains a mystery. This study, however, delves into uncharted territory by assessing the impact of ATP, a danger signal released by stressed cells that promotes a pro-inflammatory response, and adenosine, a metabolite of ATP known for its immunosuppressive role, on B-lymphocyte activation.To evaluate this, B cells were incubated with different concentrations of ATP and adenosine prior to activation with antigen-coated beads that mimic the immunological synapse with an antigen-presenting cell. Our results indicate that extracellular ATP enhances the ability of B cells to extract and present antigens. We also found that the P2X4 receptor is recruited to the immune synapse following BCR activation, mediating the effects of ATP on B cell functions by regulating actin reorganisation and promoting the cellular stretch response of B cells. In contrast, stimulation with adenosine decreases the ability of B cells to extract and present antigens.Overall, our findings suggest that the presence of ATP and adenosine in the extracellular milieu locally regulates B-cells activation and function, providing new insights into the modulation of their immune response and possible therapeutic targets for inflammatory diseases.