Browsing by Author "Villalón, Manuel J."
Now showing 1 - 20 of 52
Results Per Page
Sort Options
- Item17-Beta-Estradiol Upregulates Cox-2 in the Rat Oviduct(2006) Pérez S; Villalón, Manuel J.
- Item2-Methoxyestradiol inhibits progesterone-dependent tissue factor expression and activity in breast cancer cells(2010) Quezada, M.; Diaz, J.; Henriquez, S.; Bravo Castillo, María Loreto; Aranda, E.; Oliva, B.; Villalón, Manuel J.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Brosens, J. J.; Lange, C. A.; Owen, Gareth Ivor2-Methoxyestradiol (2ME) is an endogenous metabolite of 17β-estradiol with antiangiogenic and antitumor properties, although its mechanisms of action remain unclear. Progestins in hormone replacement therapy increase the risk of breast cancer. Progesteron
- ItemA non-genomic signaling pathway shut down by mating changes the estradiol-induced gene expression profile in the rat oviduct(2010) Parada Bustamante, Alexis Andrés.; Rios Raggio, Mariana; Villalón, Manuel J.
- ItemAcidic pH and Increasing [Ca2+] Reduce The Swelling of Mucins in Primary Cultures of Human Cervical Cells(2002) Espinosa, M.; Villalón, Manuel J.
- ItemAn autocrine ATP release mechanism regulates basal ciliary activity in airway epithelium(2017) Droguett, K.; Ríos, M.; Carreño, D.; Navarrete, C.; Fuentes, C.; Villalón, Manuel J.; Barrera Rojas, Nelson Patricio
- ItemATP and adenosine trigger the interaction of plasma membrane IP3 receptors with protein kinase A in oviductal ciliated cells(2007) Barrera Rojas, Nelson Patricio; Villalón, Manuel J.
- ItemCalcium (Ca2+) waves data calibration and analysis using image processing techniques(2013) Milovic Fabregat, Carlos Andrés; Oses, Carolina; Villalón, Manuel J.; Uribe Arancibia, Sergio A.; Prieto Vásquez, Claudia; Andía Kohnenkampf, Marcelo Edgardo; Irarrázaval Mena, Pablo; Tejos Núñez, Cristián Andrés; Lizama, CarlosAbstract Background Calcium (Ca2+) propagates within tissues serving as an important information carrier. In particular, cilia beat frequency in oviduct cells is partially regulated by Ca2+ changes. Thus, measuring the calcium density and characterizing the traveling wave plays a key role in understanding biological phenomena. However, current methods to measure propagation velocities and other wave characteristics involve several manual or time-consuming procedures. This limits the amount of information that can be extracted, and the statistical quality of the analysis. Results Our work provides a framework based on image processing procedures that enables a fast, automatic and robust characterization of data from two-filter fluorescence Ca2+ experiments. We calculate the mean velocity of the wave-front, and use theoretical models to extract meaningful parameters like wave amplitude, decay rate and time of excitation. Conclusions Measurements done by different operators showed a high degree of reproducibility. This framework is also extended to a single filter fluorescence experiments, allowing higher sampling rates, and thus an increased accuracy in velocity measurements.
- ItemCatechol-O-methyltransferase and methoxyestradiols participate in the intraoviductal nongenomic pathway through which estradiol accelerates egg transport in cycling rats(2007) Parada, Andrés; Villalón, Manuel J.; Croxatto A., Horacio
- ItemCharacterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells(2008) Kato Cardemil, Sumie Rode; Villalón, Manuel J.; Cuello F., Mauricio; Owen, Gareth Ivor; Espinoza, Natalia; Lange, Soledad
- ItemCharacterization of a Marker for Tracheal Basal Cells(1995) Aitken, ML.; Villalón, Manuel J.
- ItemCharacterization of a Marker of Differentiation for Tracheal Ciliated Cells Independent of Ciliation(1993) Aitken, ML.; Villalón, Manuel J.
- ItemClass of Skew-Distributions: Theory and Applications in Biology(2006) Barrera Rojas, Nelson Patricio; Villalón, Manuel J.
- ItemConcanavalin-A Induces Granulosa Cell Death and Inhibits FSH-Mediated Follicular Growth and Ovarian Maturation in Female Rats(2013) Velásquez Opazo, Ethel Virginia; Ríos Raggio, Mariana; Ortíz Scarlazetta, María Elena; Lizama, Carlos; Orge, Felipe; Oliva, Barbara; Orellana Walden, Renán Felipe; Villalón, Manuel J.; Moreno Mauro, Ricardo D.; Owen, Gareth Ivor; Núñez, Elizabeth; Abramovich, Dalhia; Tesone, Marta, Rokka, Anne; Corthals, Garry; Croxatto A., Horacio; Parborell, Fernanda
- ItemControl of basal ciliary activity and response to mechanical and chemical stimuli depend on extracellular ATP through an autocrine/paracrine mechanism.(2014) Droguett Quezada, Karla Denise; Villalón, Manuel J.; Pontificia Universidad Católica de Chile. Facultad de Ciencias BiológicasEn el epitelio mucociliado de las vías respiratorias y del oviducto, la frecuencia de batido ciliar (CBF) es el principal factor que determina la efectividad del transporte mucociliar. CBF se puede medir en un estado basal o incrementada por un estimulo químico o mecánico. ATP es un conocido estimulante de la CBF, efecto que es mediado por la activación de receptores purinérgicos. El incremento en la CBF inducido por ATP depende de la liberación de Ca2+ de almacenes intracelulares y de la entrada de Ca2+ desde el medio extracelular. En células del epitelio respiratorio, ATP es liberado al medio extracelular de manera constitutiva o en respuesta a una estimulación mecánica (MS), por un mecanismo que involucraría hemicanales (HCs) de panexina 1 (Panx1) o de conexina 43 (Cx43). El objetivo de este estudio fue determinar si ATP liberado por el epitelio mucociliado contribuye en el mecanismo de control de la actividad ciliar basal y si el incremento de la CBF inducido por una estimulación química y mecánica es mediado por la liberación de ATP.
- ItemControl of Ciliary Movement in Mamalian Oviductal Ciliated Cells(1991) Verdugo, P.; Villalón, Manuel J.
- ItemCultivo primario de células ciliadas de adenoides humanos. Un modelo experimental para evaluar la actividad ciliar in vitro(2007) González, Claudia.; Sánchez Dueñas, María Trinidad; Villalón, Manuel J.
- ItemEffect of Nitric Oxide Synthase Inhibitors on Ovum Transport and Oviductal Smooth Muscle Activity in the Rat Oviduct(2000) Pérez S; Villalón, Manuel J.
- ItemEffect of oestradiol and progesterone on the instant and directional velocity of microsphere movements in the rat oviduct: gap junctions mediate the kinetic effect of oestradiol(2007) Ríos, M.; Croxatto A., Horacio; Villalón, Manuel J.
- ItemEpidermal Growth Factor Prevents Prepartum Luteolysis in the Rat(2005) Ribeiro, M.; Villalón, Manuel J.
- «
- 1 (current)
- 2
- 3
- »