Browsing by Author "Vásquez Pérez, Luz Mónica"
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- ItemA Previously Unexposed Forest Soil Microbial Community Degrades High Levels of the Pollutant 2,4,6-Trichlorophenol(2004) Sánchez Zúñiga, Miguel Ángel.; Vásquez Pérez, Luz Mónica; González Ojeda, Bernardo
- ItemAscorbic acid contribution to ORAC values in berry extracts: An evaluation by the ORAC-pyrogallol red methodology(2009) Atala Riffo, Elías Duban.; Vásquez Pérez, Luz Mónica; López Alarcón, Camilo Ignacio
- ItemBioaugmentation with Pseudomonas sp strain MHP41 promotes simazine attenuation and bacterial community changes in agricultural soils(2010) Morgante, V.; Vásquez Pérez, Luz Mónica
- ItemCharacterization and identification of an iron-oxidizing, Leptospirillum-like bacterium, present in the high sulfate leaching solution of a commercial bioleaching plant(2003) Romero, Jaime.; Vásquez Pérez, Luz Mónica
- ItemCo-occurrence of microcystin and microginin congeners in Brazili82an strains of Microcystis sp.(2012) Leal Carneiro, Ronaldo; Dörr, Felipe Augusto; Dörr, Fabiane; Bortoli, Stella; Delherbe Martínez, Nathalie Andreé; Vásquez Pérez, Luz Mónica; Pinto, Ernani
- ItemComplete genome sequence of a cylindrospermopsin-producing cyanobacterium, Cylindrospermopsis raciborskii CS505, containing a circular chromosome and a single extrachromosomal element(2016) Fuentes Valdés, Juan J.; Plominsky, Alvaro M.; Allen, Eric E.; Tamames, Javier; Vásquez Pérez, Luz Mónica
- ItemCyDiv, a Conserved and Novel Filamentous Cyanobacterial Cell Division Protein Involved in Septum Localization(2016) Mandakovic, D.; Trigo, C.; Andrade, D.; Riquelme, B.; Gómez, G.; Soto, K.|Diez Moreno, Beatríz; Vásquez Pérez, Luz Mónica
- ItemDinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505(2013) Plominsky, A.; Delherbe Martínez, Nathalie Andreé.; Osses López, Igor Bastián.; Vásquez Pérez, Luz Mónica
- ItemDiurnal changes in active carbon and nitrogen pathways along the temperature gradient in porcelana hot spring microbial mat(2018) Alcamán Arias, María E.; Pedrós Alió, Carlos; Tamames, Javier; Fernández, Camila; Pérez Pantoja, Danilo; Diez Moreno, Beatriz; Vásquez Pérez, Luz Mónica
- ItemEditorial overview : Remapping the body of the world : Exploring interactions at the interface of microbial ecology, biological engineering and information science(2016) Hallam, Steven J.; Vásquez Pérez, Luz Mónica
- ItemGenomic characterization of Cylindrospermopsis raciborskii : a cyanobacterium able to produce bioactive compounds(2019) Fuentes Valdés, Juan José; Garrido Cortés, Daniel; Vásquez Pérez, Luz Mónica; Pontificia Universidad Católica de Chile. Escuela de IngenieríaLas cianobacterias son microorganismos esenciales para la vida, ya que, desarrollan el proceso de fotosíntesis y fijación de nitrógeno atmosférico. Los compuestos producidos por estos microorganismos tienen un potencial biotecnológico destacable, sin embargo, también son responsables de generar intoxicaciones graves producto del consumo de agua contaminadas, como es el caso de la intoxicación por la toxina saxitoxina. Avances en genómica y bioinformática han facilitado el descubrimiento de nuevos compuestos además de ayudar a comprender su evolución cianobacteriana y diseminación en el medio ambiente. En esta tesis, mediante un enfoque molecular se buscó e identificó un grupo de compuestos bioactivos, además de proporcionar una caracterización molecular y química de las moléculas identificadas. El primer objetivo fue obtener las secuencias genómicas de aislamientos de Cylidrospermopsis raciborskii australianos y desarrollar un análisis de genómica comparativa, para comprender su relación genética con la especie brasileña Raphidiopsis brookii, con un enfoque especial en la producción de saxitoxina. En general, observamos una fuerte correlación biogeográfica entre las cepas analizadas. La comparación genómica del grupo C. raciborskii y R. brookii mostró un alto grado de similitud en términos globales, sin embargo, se observaron diferencias significativas en términos de la biosíntesis de metabolitos secundarios. En segundo lugar, los clústeres de genes para metabolitos secundarios identificados en los genomas de Cylindrospermopsis, se estudiaron a nivel molecular. Se determinó el potencial de C. raciborskii para producir compuestos activos del tipo hassallidinas y saxitoxinas. Usando LC-MS / MS determinamos el perfil de hsasallidina en C. raciborskii CS-505, e identificamos una nueva variante de hassallidina en la cepa CS-509. Curiosamente, no logramos detectar hassallidina en extractos de C. raciborskii CS-508, a pesar de la presencia del cluster de genes aparentemente completo. Esto podría explicarse por mecanismos reguladores o mutaciones puntuales. Finalmente, determinamos el perfil de saxitoxina en la cepa C. raciborskii MVCC14, que resultó ser el mismo perfil identificado en R. brookii D9. Cylindrospermopsis raciborskii y Raphidiopsis brookii se clasifican morfológicamente como especies diferentes, mediante análisis de genómica comparativa los datos sugieren que ambos podrían ser miembros de la misma especie. En resumen, este trabajo proporcionó información importante referente a genomas cianobacterianos, determinando las estructuras genéticas responsables de la producción de metabolitos secundarios de compuestos de importancia biotecnológica como hassallidina y saxitoxina.
- ItemHigh-efficiency nuclear transformation of the microalgae Nannochloropsis oceanica using Tn5 Transposome for the generation of altered lipid accumulation phenotypes(2019) Osorio, Hector.; Jara Lagos, Carol Andrea.; Fuenzalida Morales, Karen Andrea; Rey Jurado, Emma.; Vásquez Pérez, Luz MónicaAbstract Background One of the major problems in the production of lipids for biotechnological purposes using microalgae is maintaining a high productivity of these molecules without reducing cellular biomass. High production rates are usually obtained by cultivating microalgae under different stress conditions. However, many of these changes usually result in lower biomass productivity. Therefore, the optimization of the culture conditions and genetic modification techniques in these organisms is needed to generate robust new strains for profitable economic use. Results In this work, we describe a new strategy for random mutation of genomic DNA in the microalgae Nannochloropsis oceanica by insertion of a Transposome complex Tn5. This complex contains an antibiotic-resistance cassette commanded by a CMV viral promoter that allows high efficiency of transformation and the generation of mutants. This strategy, complemented with a large-scale identification and selection system for mutants, such as flow cytometry with cell selection, allowed us to obtain clonal cultures of mutants with altered phenotypes in the accumulation of intracellular lipids. The characterization of some of these mutants uncovered new genes that are likely to be involved in the regulation of lipid synthesis, revealing possible cellular responses that influence the intracellular homeostasis of lipids. Conclusion The strategies proposed here are easy to implement in different types of microalgae and provide a promising scenario for improving biotechnological applications.Abstract Background One of the major problems in the production of lipids for biotechnological purposes using microalgae is maintaining a high productivity of these molecules without reducing cellular biomass. High production rates are usually obtained by cultivating microalgae under different stress conditions. However, many of these changes usually result in lower biomass productivity. Therefore, the optimization of the culture conditions and genetic modification techniques in these organisms is needed to generate robust new strains for profitable economic use. Results In this work, we describe a new strategy for random mutation of genomic DNA in the microalgae Nannochloropsis oceanica by insertion of a Transposome complex Tn5. This complex contains an antibiotic-resistance cassette commanded by a CMV viral promoter that allows high efficiency of transformation and the generation of mutants. This strategy, complemented with a large-scale identification and selection system for mutants, such as flow cytometry with cell selection, allowed us to obtain clonal cultures of mutants with altered phenotypes in the accumulation of intracellular lipids. The characterization of some of these mutants uncovered new genes that are likely to be involved in the regulation of lipid synthesis, revealing possible cellular responses that influence the intracellular homeostasis of lipids. Conclusion The strategies proposed here are easy to implement in different types of microalgae and provide a promising scenario for improving biotechnological applications.
- ItemImpact of nitrogen sources on gene expression and toxin production in the diazotroph Cylindrospermopsis raciborskii CS-505 and non-diazotroph Raphidiopsis brookii D9(2014) Stucken, K.; Soto Liebe, Katia Leandra.; Vásquez Pérez, Luz Mónica
- ItemIn Silico Analysis of Putative Paralytic Shellfish Poisoning Toxins Export Proteins in Cyanobacteria(2013) Soto Liebe, Katia Leandra.; Fuentes Valdés, Juan José.; Vásquez Pérez, Luz Mónica
- ItemIntercellular transfer along the trichomes of the invasive terminal heterocyst forming cyanobacterium Cylindrospermopsis raciborskii CS-505(2015) Plominsky, Álvaro M.; Delherbe, Nathalie; Mandakovic, Dinka; Riquelme del Río, Brenda Olivia; González, Karen; Bergman, Birgitta; Mariscal, Vincente; Vásquez Pérez, Luz Mónica
- ItemLatitudinal transects in the southeastern Pacific Ocean reveal a diverse but patchy distribution of phycotoxins(2011) Trefault Carrillo, Nicole Natalie.; Delherbe Martínez, Nathalie Andreé.; Vásquez Pérez, Luz Mónica
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- ItemNext Generation Sequencing and mass spectrometry reveal high taxonomic diversity and complex phytoplankton-phycotoxins patterns in Southeastern Pacific fjords(2018) Moreno-Pino, Mario; Krock, Bernd; De la Iglesia Cabezas, Rodrigo Alonso; Echenique-Subiabre, Isidora; Pizarro, Gemita; Vásquez Pérez, Luz Mónica; Trefault Carrillo, Nicole Natalie
- ItemOptimization of 2D-PAGE protocols for proteomic analysis of two nonaxenic toxin-producing freshwater cyanobacteria: Cylindrospermopsis raciborskii and Raphidiopsis sp(2009) Plominsky, Am.; Soto Liebe, Katia Leandra.; Vásquez Pérez, Luz Mónica
- ItemPharmacologic antagonism of dopamine receptor D3 attenuates neurodegeneration and motor impairment in a mouse model of Parkinson's disease(2017) Elgueta, Daniela; Aymerich, Maria S.; Contreras, Francisco; Montoya, Andro.; Celorrio, Marta; Rojo Bustamante, Estefanía; Riquelme, Eduardo; González, Hugo; Vásquez Pérez, Luz Mónica; Franco, Rafael; Pacheco, Rodrigo