Browsing by Author "Toledo, Fernando"
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- ItemConsequences of the exposome to gestational diabetes mellitus(2023) Rudge, Marilza V. C.; Alves, Fernanda C. B.; Hallur, Raghavendra L. S.; Oliveira, Rafael G.; Vega, Sofia; Reyes, David R. A.; Floriano, Juliana F.; Prudencio, Caroline B.; Garcia, Gabriela A.; Reis, Fabiana V. D. S.; Emanueli, Costanza; Fuentes, Gonzalo; Cornejo, Marcelo; Toledo, Fernando; Valenzuela-Hinrichsen, Andres; Guerra, Catalina; Grismaldo, Adriana; Valero, Paola; Barbosa, Angelica M. P.; Sobrevia, LuisThe exposome is the cumulative measure of environmental influences and associated biological responses throughout the lifespan, including those from the environment, diet, behaviour, and endogenous processes. The exposome concept and the 2030 Agenda for the Sustainable Development Goals (SDGs) from the United Nations are the basis for understanding the aetiology and consequences of non-communicable diseases, including gestational diabetes mellitus (GDM). Pregnancy may be developed in an environment with adverse factors part of the immediate internal medium for fetus development and the external medium to which the pregnant woman is exposed. The placenta is the interface between maternal and fetal compartments and acts as a protective barrier or easing agent to transfer exposome from mother to fetus. Under and over-nutrition in utero, exposure to adverse environmental pollutants such as heavy metals, endocrine-disrupting chemicals, pesticides, drugs, pharmaceuticals, lifestyle, air pollutants, and tobacco smoke plays a determinant role in the development of GDM. This phenomenon is worsened by metabolic stress postnatally, such as obesity which increases the risk of GDM and other diseases. Clinical risk factors for GDM development include its aetiology. It is proposed that knowledge-based interventions to change the potential interdependent ecto-exposome and endo-exposome could avoid the occurrence and consequences of GDM.
- ItemEscherichia coli Heat-Stable Enterotoxin Mediates Na+/H+ Exchanger 4 Inhibition Involving cAMP in T-84 Human Intestinal Epithelial Cells(2015) Beltrán, Ana R.; Carraro Lacroix, Luciene R.; Bezerra, Camila N. A.; Cornejo, Marcelo; Norambuena, Katrina; Toledo, Fernando; Araos, Joaquín; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sanhueza, Carlos; Malnic, Gerhard; Sobrevía Luarte, Luis Alberto; Ramirez, Marco A.
- ItemExtracellular Vesicles and Insulin Resistance: A Potential Interaction in Vascular Dysfunction(2019) Sáez, Tamara; Toledo, Fernando; Sobrevía Luarte, Luis Alberto
- ItemExtracellular vesicles in obesity and diabetes mellitus(2018) Pardo, Fabián Nicolás; Villalobos Labra, Roberto Esteban; Sobrevia, B.; Toledo, Fernando; Sobrevía Luarte, Luis Alberto
- ItemFoetoplacental communication via extracellular vesicles in normal pregnancy and preeclampsia(2018) Chiarello, Delia I.; Salsoso Rodríguez, M. Rocío; Toledo, Fernando; Mate, Alfonso; Vázquez, Carmen M.; Sobrevía Luarte, Luis Alberto
- ItemFoetoplacental epigenetic changes associated with maternal metabolic dysfunction(2018) Kerr, Bredford; Leiva Mendoza, Andrea Alejandra; Farías Jofré, Marcelo Enrique; Contreras Duarte, Susana de las Mercedes; Toledo, Fernando; Stolzenbach, Francisca; Silva, Luis; Sobrevía Luarte, Luis Alberto
- ItemHuman umbilical vein endothelium-derived exosomes play a role in foetoplacental endothelial dysfunction in gestational diabetes mellitus(2018) Sáez, Tamara; Salsoso Rodríguez, M. Rocío; Leiva Mendoza, Andrea Alejandra; Toledo, Fernando; de Vos, Paul; Faas, Marijke; Sobrevía Luarte, Luis Alberto
- ItemInsulin therapy and its consequences for the mother, foetus, and newborn in gestational diabetes mellitus(2018) Subiabre Morales, Mario Enrique; Silva, Luis; Toledo, Fernando; Paublo, Mario; López, Marcia A.; Boric P., Mauricio; Sobrevía Luarte, Luis Alberto
- ItemIntracellular acidification reduces L-arginine transport via system y+L but not via system y+/CATs and nitric oxide synthase activity in human umbilical vein endothelial cells(2018) Ramírez Gibert, Marco Antonio; Morales, Jorge; Cornejo, Marcelo; Blanco, Elias H.; Mancilla-Sierpe, Edgardo; Toledo, Fernando; Beltrán, Ana R.; Sobrevía Luarte, Luis Alberto
- ItemMaternal insulin therapy does not restore foetoplacental endothelial dysfunction in gestational diabetes mellitus(2017) Subiabre, Mario; Silva, Luis; Villalobos-Labra, Roberto; Toledo, Fernando; Paublo, Mario; Lopez, Marcia A.; Salsoso, Rocio; Pardo, Fabian; Leiva, Andrea; Sobrevia, LuisPregnant women diagnosed with gestational diabetes mellitus subjected to diet (GDMd) that do not reach normal glycaemia are passed to insulin therapy (GDMi). GDMd associates with increased human cationic amino acid transporter 1 (hCAT-1)-mediated transport of L-arginine and nitric oxide synthase (NOS) activity in foetoplacental vasculature, a phenomenon reversed by exogenous insulin. Whether insulin therapy results in reversal of the GDMd effect on the foetoplacental vasculature is unknown. We assayed whether insulin therapy normalizes GDMd-associated foetoplacental endothelial dysfunction. Primary cultures of human umbilical vein endothelial cells (HUVECs) from GDMi pregnancies were used to assay L-arginine transport kinetics, NOS activity, p44/42(mapk) and protein kinase B/Akt activation, and umbilical vein rings reactivity. HUVECs from GDMi or GDMd show increased hCAT-1 expression and maximal transport capacity, NOS activity, and eNOS, and p44/42(mapk), but not Akt activator phosphorylation. Dilation in response to insulin or calcitonin-gene related peptide was impaired in umbilical vein rings from GDMi and GDMd pregnancies. Incubation of HUVECs in vitro with insulin (1 nmol/L) restored hCAT-1 and eNOS expression and activity, and eNOS and p44/42(mapk) activator phosphorylation. Thus, maternal insulin therapy does not seem to reverse GDMd-associated alterations in human foetoplacental vasculature.
- ItemMechanisms of the effect of magnesium salts in preeclampsia(2018) Chiarello, Delia I.; Marín, Reinaldo; Proverbio, Fulgencio; Coronado, Paula; Toledo, Fernando; Salsoso Rodríguez, M. Rocío; Gutiérrez, Jaime; Sobrevía Luarte, Luis Alberto
- ItemPreeclampsia associates with RECK-dependent decrease in human trophoblasts migration and invasion(2017) Gutiérrez, Jaime; Aedo, Alejandro; Mora, Jacob; Maldonado, Jorge; Salsoso Rodríguez, M. Rocío; Toledo, Fernando; Farías Jofré, Marcelo Enrique; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto
- ItemRole for Tetrahydrobiopterin in the Fetoplacental Endothelial Dysfunction in Maternal Supraphysiological Hypercholesterolemia(2016) Leiva Mendoza, Andrea Alejandra; Fuenzalida Saavedra, Bárbara; Westermeier, Francisco; Toledo, Fernando; Salomón, Carlos; Gutiérrez, Jaime; Sanhueza, Carlos; Pardo, Fabián; Sobrevía Luarte, Luis Alberto
- ItemRole of extracellular vesicles in glioma progression(2018) Quezada, Claudia; Torres, Ángelo; Niechi, Ignacio; Uribe, Daniel; Contreras Duarte, Susana de las Mercedes; Toledo, Fernando; San Martín, Rody; Gutiérrez Pérez, Jaime Agustín; Sobrevía Luarte, Luis Alberto
- ItemSodium/proton exchanger isoform 1 regulates intracellular pH and cell proliferation in human ovarian cancer(2017) Sanhueza, Carlos; Araos, Joaquín; Naranjo, Luciano; Toledo, Fernando; Beltrán, Ana R.; Ramirez, Marco A.; Gutiérrez, Jaime; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto
- ItemTetrahydrobiopterin Role in human umbilical vein endothelial dysfunction in maternal supraphysiological hypercholesterolemia(2016) Leiva Mendoza, Andrea Alejandra; Fuenzalida Saavedra, Bárbara; Salsoso Rodríguez, M. Rocío; Barros, Eric; Toledo, Fernando; Gutiérrez, Jaime; Pardo, Fabián; Sobrevía Luarte, Luis Alberto