Browsing by Author "Smith, Patricio C."
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- ItemAgeing, dental caries and periodontal diseases(2017) López, R.; Smith, Patricio C.; Göstemeyer, G.; Schwendicke, F.
- Itemc-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells(2014) Arancibia, Rodrigo.; Smith, Patricio C.; Tobar, Nicolás.; Toyos, Marcela.; Urra, Carla.; Méndez, Nicolás.; Martínez, Jorge.Abstract Background Hard consistency, developed under the influence of tumor cell factors, is a characteristic feature of a breast tumor. Activation of resident fibroblasts leading to a myofibroblast phenotype is the principal feature that orchestrates this fibrotic process. The aim of this study was to assess the effects induced by TGF-β1, a growth factor abundantly present in tumor microenvironment, on the molecular mechanisms that mediate myofibroblastic differentiation of normal human mammary fibroblasts. Methods We used an immortalized fibroblastic cell line derived from normal mammary tissue (RMF-EG cells) to study the effect of TGF-β1 in the expression of α-SMA and CTGF as markers of myofibroblastic differentiation. The influence of redox status and JNK activity on TGF-β1-induced transcriptional activity was measured by a luciferase reporter assay. We also used a shRNA approach to evaluate the influence of NOX4 in myofibroblastic differentiation. Results TGF-β1 stimulates the expression of myofibroblast markers α-SMA and CTGF. Using a NOX inhibitor (DPI) and cells expressing a shRNA for NOX4, we demonstrated that TGF-β1 promotes an oxidative environment that favors myofibroblastic differentiation. We also found that activation of c-Jun N-terminal kinase is required for TGF-β1-dependent expression of CTGF, NOX4 and α-SMA. Conclusions Human mammary stromal fibrosis, evaluated by the expression of early and late markers as CTGF and α-SMA, depends on the activation of JNK signaling pathway. Our results show that JNK activation is an early event that precedes the increase in ROS levels leading to myofibroblastic differentiation and tumor fibrosis, suggesting that inhibition of JNK may be used a method to interrupt the development of tumor desmoplasia.
- ItemChitosan and platelet-derived growth factor synergistically stimulate cell proliferation in gingival fibroblasts(2013) Silva, Daniel; Arancibia, Rodrigo; Tapia, Cristián; Acuña Rougier, Cristina; Díaz Dosque, Mario; Cáceres, Mónica; Martínez, J.; Smith, Patricio C.
- ItemDefective Wound-healing in Aging Gingival Tissue(2014) Cáceres, M.; Oyarzún, A.; Smith, Patricio C.
- ItemEffects of Chitosan Particles in Periodontal Pathogens and Gingival Fibroblasts(2013) Arancibia Reyes, Rodrigo Esteban; Maturana Salgado, Cristián Roberto; Silva Vargas, Daniel Ignacio; Tobar Chler, Nicólas Andrés; Tapia Villanueva, Cristián Adolfo; Salazar, Juan C.; Martínez Winkler, Jorge; Smith, Patricio C.
- ItemGalectin-8 induces partial epithelial–mesenchymal transition with invasive tumorigenic capabilities involving a FAK/EGFR/proteasome pathway in Madin–Darby canine kidney cells(2018) Oyanadel, Claudia; Holmes Videla, Christopher Edward; Pardo Huguet, Evelyn Cristina; Retamal Villarroel, Claudio Enrique; Shaughnessy, Ronan Patrick; Smith, Patricio C.; Cortés Martínez, Priscilla Rocío; Bravo Zehnder, Marcela; Metz Baer, Claudia Andrea; Feuerhake, Teo; Romero, Diego; Roa Strauch, Juan Carlos Enrique; Montecinos, Viviana; Soza Gajardo, Andrea; González, Alfonso
- ItemGlucose Promotes a Pro‐Oxidant and Pro‐Inflammatory Stromal Microenvironment Which Favors Motile Properties in Breast Tumor Cells(2017) Kallens, Violeta; Tobar, Nicolás; Molina, Jessica; Bidegain, Arantzazú; Smith, Patricio C.; Porras, Omar; Martínez, Jorge
- ItemGlycated Collagen Stimulates Differentiation of Gingival Myofibroblasts.(2017) Retamal, Ignacio N.; Hernández, Romina; Melo Ledermann, Francisco Javier; Zapata, Paulina; Martinez, Constanza; Martínez, Jorge; Smith, Patricio C.
- ItemModulation of Mammary Stromal Cell Lactate Dynamics by Ambient Glucose and Epithelial Factors(2017) Tobar, N; Porras, O.; Smith, Patricio C.; Barros, L.; Martinez, J.
- ItemResearch on growth factors in periodontology(2015) Smith, Patricio C.; Martínez, Constanza; Cáceres, Mónica; Martínez Castillo, Jorge
- ItemRole of myofibroblasts in normal and pathological periodontal wound healing(2018) Smith, Patricio C.
- ItemSoluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells(2014) Tobar, N.; Avalos M., Celeste; Mendez, N; Smith, Patricio C.; Bernabeu, C.; Quintanilla, M.; Martínez, J.
- ItemTumor Necrosis Factor-alpha Inhibits Transforming Growth Factor-beta-Stimulated Myofibroblastic Differentiation and Extracellular Matrix Production in Human Gingival Fibroblasts(2013) Arancibia, R.; Oyarzún, A.; Silva, D.; Tobar, N.; Martínez, J.; Smith, Patricio C.