Browsing by Author "Sjoholm, I."
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- ItemAn experimental set-up for studying acrylamide formation in potato crisps(2007) Viklund, G.; Mendoza, F.; Sjoholm, I.; Skog, K.The objective of this work was to set up lab-scale equipment for production of crisps mimicking industrial conditions. Slices of Saturna potatoes were deep-fat fried for 2-4.5 min at 160 degrees C. A solid phase extraction method for acrylamide from potato crisps was used, and the extraction recovery was calculated to 95%. Acrylamide was analysed using liquid chromatography tandem mass spectrometry. The relative standard deviation was below 3% for analyses performed on the same day and below 5% for inter-day analyses. The limit of quantification was estimated to be 160 mu g/kg potato crisps. The colour of potato slices was determined using a digital imaging method and related to the acrylamide content. There were tendencies that L*(lightness) decreased and that that a*(redness) and b*(yellowness) increased with increasing acrylamide content. In another experiment, potatoes with different glucose levels were fried for 4 min but no significant difference in acrylamide content (2200-2800 mu g/kg) was observed. The experiment was repeated after three months of storage. The levels of acrylamide increased significantly to 8200-13200 mu g/kg. The potatoes had been fertilized with different levels of nitrogen, but no relation was found between the nitrogen supplied and the acrylamide content. The experimental set-up was shown to give realistic and reproducible experimental data, regarding colour, water content and acrylamide levels. It will be used together with the analytical methods as a platform for further research on the formation of acrylamide. (c) 2006 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.
