Browsing by Author "Shoji, Kenji F."
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- ItemATP is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro(2013) Sáez, Pablo J.; Shoji, Kenji F.; Retamal, Mauricio A.; Harcha, Paloma A.; Ramírez, Ggigliola; Jiang, Jean X.; Bernhardi Montgomery, Rommy von; Sáez, Juan Carlos
- ItemATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X(7) receptors(2017) Sáez, Pablo J; Vargas, Pablo; Shoji, Kenji F.; Harcha, Paloma A.; Lennon-Duménil, Ana María; Sáez, Juan Carlos
- ItemCannabinoids Prevent the Amyloid beta-Induced Activation of Astroglial Hemichannels: A Neuroprotective Mechanism(2017) Gajardo Gómez, Rosario; Labra, Valeria C.; Maturana, Carola J.; Shoji, Kenji F.; Sáez, Juan Carlos; Orellana Roca, Juan Andrés
- ItemConnexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+](i) responses(AMER SOC CELL BIOLOGY, 2008) Schalper, Kurt A.; Palacios Prado, Nicolas; Retamal, Mauricio A.; Shoji, Kenji F.; Martinez, Agustin D.; Saez, Juan C.Cell surface hemichannels (HCs) composed of different connexin (Cx) types are present in diverse cells and their possible role on FGF-1-induced cellular responses remains unknown. Here, we show that FGF-1 transiently (4-14 h, maximal at 7 h) increases the membrane permeability through HCs in HeLa cells expressing Cx43 or Cx45 under physiological extracellular Ca2+/Mg2+ concentrations. The effect does not occur in HeLa cells expressing HCs constituted of Cx26 or Cx43 with its C-terminus truncated at aa 257, or in parental nontransfected HeLa cells. The increase in membrane permeability is associated with a rise in HC levels at the cell surface and a proportional increase in HC unitary events. The response requires an early intracellular free Ca2+ concentration increase, activation of a p38 MAP kinase-dependent pathway, and a regulatory site of Cx subunit C-terminus. The FGF-1-induced rise in membrane permeability is also associated with a late increase in intracellular free Ca2+ concentration, suggesting that responsive HCs allow Ca2+ influx. The cell density of Cx26 and Cx43 HeLa transfectants cultured in serum-free medium was differentially affected by FGF-1. Thus, the FGF-1-induced cell permeabilization and derived consequences depend on the Cx composition of HCs.
- ItemPannexin1 channels act downstream of P2X 7 receptors in ATP-induced murine T-cell death(2014) Shoji, Kenji F.; Sáez Pedraza, Pablo José; Harcha, Paloma A.; Aguila, Hector L.; Sáez, Juan Carlos