Browsing by Author "Serrano Honeyman, Carolina"

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    Caregiver's depressive symptoms and asthma control in children from an underserved community
    (2017) Rioseco Castillo, Andrea; Serrano Honeyman, Carolina; Celedón, Juan C.; Padilla Pérez, Oslando; Puschel Illanes, Klaus; Castro Rodríguez, José Antonio
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    Conversion to mycophenolate mofetil monotherapy in liver recipients: Calcineurin inhibitor levels are key
    (2017) Norero, Blanca; Serrano Honeyman, Carolina; Sánchez Fueyo, A.; Duarte, Ignacio; Torres Montes, Paula Javiera; Ocqueteau Tachini, Mauricio; Barrera Martínez, Francisco José; Arrese Jiménez, Marco; Soza, Alejandro; Benítez, Carlos
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    Desarrollo de la Microbiota Gastrointestinal en Lactantes y Su Rol en Salud y Enfermedad
    (2016) Serrano Honeyman, Carolina; León, M. A.; Harris D., Paul R.; Serrano Honeyman, Carolina; León, M. A.; Harris D., Paul R.
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    Desarrollo del microbioma intestinal en niños: impacto en salud y enfermedad
    (2016) Serrano Honeyman, Carolina; Harris D., Paul R.; Serrano Honeyman, Carolina; Harris D., Paul R.
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    Downregulated Th17 responses are associated with reduced gastritis in Helicobacter pylori-infected children
    (2013) Serrano Honeyman, Carolina; Venegas Muñoz, Alejandro Andrés; Harris D., Paul R.; Serrano Honeyman, Carolina; Venegas Muñoz, Alejandro Andrés; Harris D., Paul R.
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    Early origins of allergy and asthma (ARIES): study protocol for a prospective prenatal birth cohort in Chile.
    (2020) Hernández Vargas, Caroll Daffner; Casanello Toledo, Paola Cecilia; Harris D., Paul R.; Castro Rodríguez, José Antonio; Iturriaga, Carolina; Pérez Mateluna, Guillermo; Farías Jofré, Marcelo Enrique; Urzúa, Marcela; Hernández Carreño, Cherie Francisca; Serrano Honeyman, Carolina; Hernández Vargas, Caroll Daffner; Casanello Toledo, Paola Cecilia; Harris D., Paul R.; Castro Rodríguez, José Antonio; Iturriaga, Carolina; Pérez Mateluna, Guillermo; Farías Jofré, Marcelo Enrique; Urzúa, Marcela; Hernández Carreño, Cherie Francisca; Serrano Honeyman, Carolina
    Abstract Background Growing evidence shows that atopic dermatitis (AD), food allergy (FA), allergic rhinitis, and asthma are largely determined during the first 1000 days (time elapsed from conception to the 2nd birthday). The ARIES birth cohort aims to determine prenatal and perinatal conditions, as well as genetic and epigenetic factors, that participate in the early setting of immune responses, and the role of these in the later determination of the risk of allergic diseases and asthma in the offspring. Methods We have designed a birth cohort of 250 families with prenatal recruitment (~ 14 weeks). We will genotype relevant allergy/asthma-associated variants in trios and will perform immunophenotyping and evaluation of allergy biomarkers in cord blood. At 1 and 2 years of age we will assess if infants have developed allergic sensitization, AD, FA, as well as biomarkers of asthma including the asthma predictive index. We will also evaluate how maternal conditions modify immune programming through epigenetic modifications and will then depict newborn epigenetic cues of allergy/asthma risk. Next, we will assess composition/diversity of maternal gut, placenta, breastmilk and infant gut microbiome and their association with immunophenotype and biomarkers at birth, and clinical outcomes at age 1 and 2. Finally, we plan to assess how environmental exposures (perinatal outdoor and indoor pollution, allergens and endotoxin) affect the incidence of allergic sensitization, AD, FA, and risk of asthma. Discussion The in-depth study of the ARIES birth cohort shall provide crucial information to understand the rising incidence of allergies and asthma in developing countries, and hopefully provide cues on how to prevent and treat these diseases. Trial registration clinicaltrials.gov NCT04186949, retrospectively registered on December 5, 2019.
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    Eradication of Helicobacter pylori in Children Restores the Structure of the Gastric Bacterial Community to That of Noninfected Children
    (2019) Serrano Honeyman, Carolina; Pierre, Reinaldo; Van Der Pol, William J.; Morrow, Casey D.; Smith, Phillip D.; Harris D., Paul R.; Serrano Honeyman, Carolina; Pierre, Reinaldo; Van Der Pol, William J.; Morrow, Casey D.; Smith, Phillip D.; Harris D., Paul R.
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    Functional gastrointestinal disorders in children from low socio-economic status and Helicobacter pylori infection
    (2018) Jaime Méndez, María Francisca; Villagrán Torres, Andrea Alejandra; Hernández Rocha, Cristián Antonio; Ortiz Aparicio, Freddy Miguel; Serrano Honeyman, Carolina; Harris Diez, Paul Richard
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    Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana
    (2008) Serrano Honeyman, Carolina; González Cruz, Javiera; Jauregui, Francisca; Medina, Consuelo; Mancilla Oyarzo, Pablo Eduardo; Matus Picero, José Tomás; Arce Johnson, Jorge Patricio
    Abstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.Abstract Background Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes. We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied. Results TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology. Conclusion The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.
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    Helicobacter pylori infection and UBT-13C values are associated with changes in body mass index in children and adults
    (2022) Bruera, María J.; Amezquita García, María Virginia; Riquelme Pérez, Arnoldo; Serrano Honeyman, Carolina; Harris D., Paul R.
    Background: The urea breath test (UBT-13C) is a non-invasive technique that allows the diagnosis and confirmation of eradication of Helicobacter pylori infection. Aim: To evaluate H. pylori positivity and values of UBT-13C among infected Chilean children and adults, and to analyze its variation in relation to sex, nutritional status, and age of the patients. Material and Methods: Retrospective study of 1141 patients aged 6 to 94 years, with an indication for a UBT-13C either for diagnosis or for confirmation of eradication of H. pylori infection. 13C enrichment was measured using an infrared spectrometer calculating the delta 13C values before and after the ingestion of 13C marked urea. The clinical data of the patients were obtained at the time of the examination. Results: We included 241 children and 900 adults. Infected children obtained lower UBT-13C delta values than infected adults (16.1 ± 8.7 and 37 ± 52.9, respectively). The rates of infection were higher in males who were recruited for diagnosis. Significant differences were obtained between positivity for H. pylori in overweight and obese children but not adults. UBT-13C titers were significantly associated with the body mass index (BMI) only in adults. Conclusions: H. pylori infection rates are similar between sexes and are higher in children probably because of selection bias. In children, H. pylori positivity is associated with higher BMI and excess malnutrition although with similar UBT-13C values. In adults, H. pylori infection is not related with BMI, but a higher BMI impacts UBT-13C titers.
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    Helicobacter pylori infection is associated with an altered gastric microbiota in children
    (2017) Brawner, K.; Kumar, R.; Serrano Honeyman, Carolina; Ptacek, T.; Lefkowitz, E.; Morrow, C.; Zhi, D.; Kyanam, K.; Smythies, L.; Harris D., Paul R.; Smith, P.; Brawner, K.; Kumar, R.; Serrano Honeyman, Carolina; Ptacek, T.; Lefkowitz, E.; Morrow, C.; Zhi, D.; Kyanam, K.; Smythies, L.; Harris D., Paul R.; Smith, P.
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    Helicobacter pylori Infection Is Associated with Decreased Expression of SLC5A8, a Cancer Suppressor Gene, in Young Children
    (2016) Orellana Manzano, Andrea; O’Ryan, Miguel G.; Lagomarcino, Anne J.; George, Sergio; Muñoz, Mindy S.; Mamani, Nora; Serrano Honeyman, Carolina; Harris D., Paul R.; Ramilo, Octavio; Mejías, Asunción; Torres, Juan P.; Lucero, Yalda; Quest, Andrew F. G.; Orellana Manzano, Andrea; O’Ryan, Miguel G.; Lagomarcino, Anne J.; George, Sergio; Muñoz, Mindy S.; Mamani, Nora; Serrano Honeyman, Carolina; Harris D., Paul R.; Ramilo, Octavio; Mejías, Asunción; Torres, Juan P.; Lucero, Yalda; Quest, Andrew F. G.
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    Helicobacter pylori pediatric infection changes Fc epsilon RI expression in dendritic cells and Treg profile in vivo and in vitro
    (2019) Leon, M.A.; Palma, C.; Hernandez, C.; Sandoval, M.; Cofre, C.; Pérez Mateluna, G.; Borzutzky Schachter, Arturo; Harris D., Paul R.; Serrano Honeyman, Carolina
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    Helicobacter pylori vacA virulence factor in uncultured Helicobacter heilmannii sensu lato from an infected child
    (2016) Hernandez, Caroll; Serrano Honeyman, Carolina; Villagran, Andrea; Torres Montes, Paula Javiera; Venegas, Alejandro; Harris D., Paul R.; Hernandez, Caroll; Serrano Honeyman, Carolina; Villagran, Andrea; Torres Montes, Paula Javiera; Venegas, Alejandro; Harris D., Paul R.
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    Helicobacter pylori-associated hypochlorhydria in children, and development of iron deficiency
    (2013) Harris D., Paul R.; Serrano Honeyman, Carolina; Villagrán Torres, Andrea Alejandra; Walker, Marjorie M.; Thomson, Melanie; Duarte, Ignacio; Windle, Henry J.; Crabtree, Jean E.
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    Helicobacter pylori-Clarithromycin Resistance in Symptomatic Pediatric Patients in a High Prevalence Country
    (2017) Serrano Honeyman, Carolina; Leon, Miguel A.; Palma, Camila; Vera, Macarena; Hernandez, Caroll; Harris D., Paul R.; Serrano Honeyman, Carolina; Leon, Miguel A.; Palma, Camila; Vera, Macarena; Hernandez, Caroll; Harris D., Paul R.
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    Helicobacter pylori: una causa no tradicional de deficiencia de hierro y anemia
    (2012) Serrano Honeyman, Carolina; Villagrán T., Andrea; Harris D., Paul R.
    Helicobacter Pylori (H. pylori) is the most common cause for chronic gastritis, duodenal ulcers, MALT lymphoma and gastric cancer. H. pylori has also been associated with several extra gastric diseases such as iron deficiency (ID) and iron deficiency anemia (IDA). Several mechanisms by which H. pylori might contribute to the development of ID/IDA have been proposed. Alterations in the physiology of gastric mucosa and of iron metabolism as a consequence of the infection as well as direct competition for iron by H. pylori have been proposed as possible causes for ID/IDA development. H. pylori causes alterations in gastric pH as well as in the concentration of ascorbic acid in the stomach limiting the absorption of dietary iron in the gastrointestinal tract. In addition, pro-inflammatory cytokine secretion in response to the infection, in particular IL-1β are able to modulate acid secretion and stimulate the secretion of hepcidin from the liver inhibiting liberation of iron from iron stores. These results in less iron available for eritropoyesis, thus contributing to ID/IDA development. Several studies have suggested a direct relationship between the presence of the infection and the development of ID/IDA in children, adolescents and adults. H. pylori eradication has been associated to a better response to oral iron therapy in IDA patients. However some controversy still remains regarding the role of the infection in ID/IDA and the mechanisms involved. Studies aimed to evaluate the relationship between H. pylori and acid output in gastric mucosa, as well as studies to determine iron capture in strains isolated from IDA patients are still being conducted in order the shed some definitive light in the relationship between this bacterium and ID/IDA and their contributing mechanisms.
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    High Helicobacter pylori Bacterial Load and Low Cytokine Expression Levels Are Associated with Nodular Gastropathy
    (2020) Mansilla-Vivar, R.; Serrano Honeyman, Carolina; Palma, C.; Vera, M.; Hernandez, C.; Pizarro Rojas, Margarita Alicia; Torres Montes, Paula Javiera; Harris D., Paul R.; Fuentes López, Eduardo; Riquelme Pérez, Arnoldo; Espino Espino, Alberto Antonio; Mansilla-Vivar, R.; Serrano Honeyman, Carolina; Palma, C.; Vera, M.; Hernandez, C.; Pizarro Rojas, Margarita Alicia; Torres, Javiera; Harris D., Paul R.; Fuentes López, Eduardo; Riquelme Pérez, Arnoldo; Espino Espino, Alberto Antonio
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    La infección materna por Helicobacter pylori no aumenta el riesgo de contraer la bacteria en el primer trimestre de vida de sus lactantes
    (2016) Troncoso, Paula; Villagrán, Andrea; Vera, Macarena; Estay Navarrete, Alberto Santiago; Ortiz, Marlene; Serrano Honeyman, Carolina; Hernández Vargas, Caroll Daffner; Harris D., Paul R.; Troncoso, Paula; Villagrán, Andrea; Vera, Macarena; Estay Navarrete, Alberto Santiago; Ortiz, Marlene; Serrano Honeyman, Carolina; Hernández Vargas, Caroll Daffner; Harris D., Paul R.
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    Iron Deficiency and IL1 beta Polymorphisms in Helicobacter pylori-infected Children
    (2016) Serrano Honeyman, Carolina; Villagran, Andrea; Toledo, H.; Crabtree, J.; Harris D., Paul R.; Serrano Honeyman, Carolina; Villagran, A.; Toledo, H.; Crabtree, J.; Harris D., Paul R.