Browsing by Author "Serrano, C."

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    Helicobacter pylori Gastritis in Children Is Associated With a Regulatory T-Cell Response
    (2008) Harris, P.R.; Wright, S.W.; Serrano, C.; Riera, F.; Duarte, I.; Torres, J.; Peña, A.; Rollán, A.; Viviani, P.; Guiraldes, E.; Schmitz, J.M.; Lorenz, R.G.; Novak, L.; Smythies, L.E.; Smith, P.D.
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    Helicobacter pylori-associated hypochlorhydria in children relates to the development of iron deficiency
    (2010) Harris, Paul R.; Villagran, A.; Serrano, C.; Duarte, I.; Windle, H. J.; Crabtree, J. E.
    Introduction Acute H pylori infection is associated with transient hypochlorhydria of variable duration. Hypochlorhydria in H pylori-associated atrophy has a role in iron deficiency (ID) through changes in the physiology of iron absorption. The role of hypochlorhydria in H pylori-associated ID in childhood has not been investigated. The aims were to evaluate the association between H pylori-associated hypochlorhydria and ID in children. Methods A total of 123 children were prospectively enrolled in the study. Any child with peptic ulceration, or who received antacids, PPIs, H2 antagonists, and antibiotics in the previous 4 weeks was excluded. Blood was taken for complete blood count and iron profile. Gastric biopsies were taken for H. pylori determination by histology and rapid urease test. H pylori status was defined as +ve if either test was positive. Gastric juice was obtained at the beginning of the endoscopy avoiding water insufflation. Duodenal biopsies were taken for exclusion of coeliac disease (CD). Stool samples were collected for parasitology/microbiology. Of the 123 children, 2 with CD, 8 with non-specific duodenal inflammation (DI), 5 with lost blood analysis and 9 with parasitic infections (2 with DI) were excluded. Results Of the remaining 101 children 31 were H pylori +ve and 10 were hypochlorhydric (pH>4). There were no differences in demographic characteristics with regard to infection status, or gastric pH. In H pylori +ve children with pH >4 (n=6) serum iron (median (IQR): 60.2 (45–66) μg/dl) and transferrin saturation levels percentage (16.6 (15–19)) were significantly lower (p<0.01) than H pylori positive children (n=25) with pH≤4 (iron 113.2 (91–132); transferrin saturation 32.6 (24–38)). No differences in ferritin or TIBC were observed. In H pylori negative children with pH>4 (n=4) both iron (119 (101–137)), and transferrin saturation (36.4 (31–41)), were not significantly different from children with pH≤4 (n=66) (iron 98.7 (80–122); transferrin saturation 32.2 (25–40)). Conclusion: Low serum iron and transferrin in childhood H pylori infection is associated with hypochlorhydria. In uninfected children, hypochlorhydria was not associated with altered serum iron parameters, indicating a combination of H pylori infection and/or inflammation and hypochlorhydria has a role in the aetiology of iron deficiency. Even though H pylori-associated hypochlorhydria may reflect a transient period during acute gastritis, it alters iron homeostasis with clinical impact in developing countries with a high H pylori prevalence. Funded by EU CONTENT Project (INCO-CT-2006-032136) and CONICYT/BM (RUE #29).
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    High prevalence of undiagnosed liver cirrhosis and advanced fibrosis in type 2 diabetic patients
    (2016) Arab Verdugo, Juan Pablo; Barrera Martínez, Francisco José; Gallego C.; Valderas Igor, Juan Patricio; Uribe Arancibia, Sergio A.; Tejos Núñez, Cristián Andrés; Serrano, C.; Huete, Isidro; Liberona, J.; Labbé P.; Quiroga, T.; Benitez, Carlos; Irarrázaval Mena, Pablo; Riquelme, A.; Arrese Jiménez, Marco
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    Medición volumétrica de grasa visceral abdominal con RM y su relación con elastografía hepática en una población diabética
    (2012) Serrano, C.; Barrera Martínez, Francisco José; Riquelme Pizarro, Carlos Rodrigo; Vidal, H.; Labbé, P.; Cruz, F.; Arrese Jiménez, Marco; Irarrázaval Mena, Pablo; Tejos, C.; Uribe, S.
    Introducción. La acumulación de grasa visceral se asocia al desarrollo de enfermedad hepática. La elastografía hepática es un método novedoso no invasivo para evaluar fibrosis hepática. Objetivo. Evaluar la relación entre el volumen de tejido adiposo visceral (VAT), volumen de tejido adiposo subcutáneo (SAT) medido por resonancia magnética (RM), con índices de elastografía hepática (EH) utilizando ARFI (fuerza de impulso de radiación acústica) en pacientes con Diabetes Mellitus tipo 2 (DM2). Métodos. Fueron incluidos 20 pacientes (edad promedio: 62 años, rango: 55-75 años, peso promedio: 77,8 kg, rango: 61,5-97 kg) con DM2. Los pacientes se sometieron a un examen de RM en un resonador Philips Intera 1.5T. Al protocolo de RM se agregó una secuencia de excitación espectral centrada en el peak de grasa. La secuencia incluyó 32 cortes transversales, grosor 7mm, desde la cúpula diafragmática hasta el borde inferior renal. En las imágenes se midió VAT utilizando el software Image J (freeware). En cada paciente se realizó una EH, utilizando ARFI con 10 medidas en lóbulo hepático derecho. Finalmente, se realizó un análisis estadístico a través de la correlación de Pearson entre los volúmenes de grasa abdominal y ARFI. Resultados. El promedio de VAT fue 2472 ± 861 cc, (1173-4020 cc), el promedio de ARFI fue 1,62 ± 0,8 m/s, (0,8-3,4 m/s). Se obtuvieron correlaciones de r=-0,08 entre VAT y ARFI (p=0,72), de 0,13 entre SAT y ARFI (p=0,57), y de -0,06 entre (VAT+SAT) con ARFI (p=0,77). Subdividiendo el universo muestral, se encontró que el grupo con ARFI mayor que 1,6 m/s (7 pacientes) obtuvo una correlación de 0,63 entre VAT y ARFI (p=0,12), de 0,66 entre SAT y ARFI (p=0,10), y de 0,94 entre (VAT+SAT) con ARFI (p=0,001). En el subgrupo con ARFI inferior a 1,6 m/s (13 pacientes) la correlación fue 0,11 entre VAT y ARFI (p=0,71), de 0,26 entre SAT y ARFI (p=0,38), y de 0,32 entre (VAT+SAT) y ARFI (p=0,28). Al ajustar por género en el subgrupo de mujeres con ARFI mayor que 1,6 m/s (6 pacientes), se encontró que la correlación de ARFI y VAT fue de 0,79 (p=0,05), y de 0,92 (p=0,009) para VAT+SAT. Conclusiones. En los pacientes DM2 con índices ARFI superior a 1,6 m/s habría una buena correlación con VAT y VAT+ SAT, indicando que aquellos con mayor daño hepático tendrían más acumulación de grasa abdominal.
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    Relationship between Helicobacter pylori virulence factors and regulatory cytokines as predictors of clinical outcome
    (2007) Serrano, C.; Díaz Morales, María Inés; Venegas Esparza, Hector Alejandro; Harris D., Paul R.
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    Relevance of adjusted cut-off values in commercial serological immunoassays for Helicobacter pylori infection in children
    (2005) Harris, Paul R.; Perez-Perez, G.; Zylberberg, A.; Rollán, A.; Serrano, C.; Riera, F.; Einisman, H.; García, D.; Viviani, P.
    We assessed the sensitivity and specificity of H. pylori IgG and IgA with a commercial immunoassay performed in Chile and a second non-commercial immunoassay performed in a reference laboratory in the United States, in serum of 80 children and adults referred for gastrointestinal endoscopies in a developing country. Overall, 56% of the patients were infected with H. pylori based on rapid urease test and staining techniques on gastric biopsies. When Receiver Operator Curves (ROC) were developed, the sensitivity and specificity were similar for IgG and IgA. Both immunoassays exhibited better specificity, positive and negative predictive value (NPV) in children than in adults when cut-off values were corrected according to the local population than when they were assessed using the cut-off values pre-defined in other populations. These results underline the need to establish more precise cut-off values corrected in the local populations where assessments of antibodies as diagnostic markers of H. pylori infection are planning. © 2005 Springer Science+Business Media, Inc.