Browsing by Author "Sáez Steeger, Claudia"

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    Effect of Tamoxifen and Retinoic Acid on Bradykinin Induced Proliferation in MCF-7 Cells
    (2009) Paola Searovic; Alonso, Marcelo; Oses, Carolina; Pereira Flores, K.; Velarde Aliaga, María Victoria; Sáez Steeger, Claudia
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    Regional brain perfusion Is associated with endothelial dysfunction markers in major depressive disorder
    (2020) Massardo, T.; Quintana Fresno, Juan Carlos; Jaimovich, Rodrigo; Sáez Steeger, Claudia; Risco, L.; Liberman, C.; Araya, A. V.; Galleguillos, T.; Pereira Garcés, Jaime Ignacio; Castro-Mora, G.
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    Vernix caseosa reveals mechanistic clues linking maternal obesity to atopic dermatitis pathogenesis
    (2023) Arenas Cabalín, Carolina Andrea; Dibarrart Duhalde, Marisol; Núñez Rosales, Juan José; Faunes Pérez, Miriam Elizabeth; Avaca Bengochea, Mónica; Ávalos Odano, Patricia De Las Mercedes; Fabres Biggs, Jorge Guillermo Eduardo; Álvarez Figueroa, María Javiera; Vera Kellet, Cristián Andrés; Silva Valenzuela, Sergio; Sáez Steeger, Claudia; Borzutzky Schachter, Arturo José
    Background Maternal overweight and obesity have been associated with an increased risk of atopic dermatitis (AD) in the offspring, but the underlying mechanisms are unclear. Vernix caseosa (VC) is a proteolipid material covering the fetus produced during skin development. However, whether maternal prepregnancy weight excess influences fetal skin development is unknown. Characterizing the VC of newborns from mothers with prepregnancy overweight and obesity might reveal AD-prone alterations during fetal skin development.ObjectiveWe sought to explore AD biomarkers and staphylococcal loads in VC from the offspring of mothers who were overweight/obese (O/O) before pregnancy versus in those from offspring of normal weight mothers.MethodsThe VC of newborns of 14 O/O and 12 normal weight mothers were collected immediately after birth. Biomarkers were determined by ELISA and staphylococcal species by quantitative PCR.ResultsThe VC from the O/O group showed decreased expression of skin barrier proteins (filaggrin and loricrin) and increased levels of proinflammatory biomarkers (IgA, thymic stromal lymphopoietin [TSLP], S100A8, IL-25, and IL-33). No differences in concentrations of antimicrobial peptides and enzymes were detected. The VC from the O/O group had a lower Staphylococcus epidermidis and Staphylococcus hominis commensal bacterial load, whereas Staphylococcus aureus bacterial load was not significantly different between the 2 groups. Maternal body mass index was negatively correlated with VC filaggrin expression and S epidermidis load and was positively associated with TSLP concentration. One-year follow-up established that the offspring of O/O mothers had a higher incidence of AD that was specifically linked with decreased VC filaggrin expression and lower S epidermidis load.ConclusionsVC from neonates of mothers with prepregnancy overweight and obesity exhibit skin barrier molecular alterations and staphylococcal dysbiosis that suggest early mechanistic clues to this population’s increased risk of AD.