Browsing by Author "Rozas Rojas, Pablo"
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- ItemDevelopment of a Hibernation-Inspired Preservation Strategy to Enhance the Clinical Translation of Photosynthetic Biomaterials(2023) Corrales-Orovio, Rocio; Castillo, Valentina; Rozas Rojas, Pablo; Schenck, Thilo L.; Egaña, José TomasPhotosynthetic biomaterials have emerged as a promising approach for delivering oxygen and other bioactive molecules in several biomedical applications. This technology is based on the use of standard biomaterials loaded with photosynthetic cells for the controlled release of oxygen at the target site. However, as well as for other cell-based approaches, a main drawback for their clinical translation is the low shelf-life of living materials. Here, the potential of inducing a dormant hibernation-inspired state to preserve photosynthetic biomaterials for clinical applications is explored. First, a protocol to preserve microalgae Chlamydomonas reinhardtii is optimized and then applied to photosynthetic scaffolds, showing that the viability and functionality of the biomaterial is preserved for up to 6 weeks. To evaluate the clinical viability of this approach, both fresh and preserved photosynthetic scaffolds are implanted in a full-skin defect mouse model. The safety of this approach is evaluated and confirmed by several means, including clinical parameters, histological assays, and local and systemic molecular analysis. Altogether, for the first time the successful preservation of photosynthetic biomaterials through a hibernation-inspired strategy is described here, which could have a tremendous impact for the clinical translation of these materials as well as other photosynthetic therapies., Photosynthetic biomaterials have garnered attention for local and controlled delivery of oxygen to tissues. A novel hibernation-inspired preservation method is presented, extending the lifespan of photosynthetic scaffolds. The research demonstrates successful preservation for up to 6 weeks without cryopreservation agents, demonstrating in vivo safety and applicability. This advancement significantly enhances the clinical potential of photosynthetic materials and related therapies.image
- ItemGenetically modifed organisms: adapting regulatory frameworks for evolving genome editing technologies(2022) Rozas Rojas, Pablo; Kessi-Pérez, Eduardo I.; Martínez, ClaudioGenetic modification of living organisms has been a prosperous activity for research and development of agricultural, industrial and biomedical applications. Three decades have passed since the first genetically modified products, obtained by transgenesis, become available to the market. The regulatory frameworks across the world have not been able to keep up to date with new technologies, monitoring and safety concerns. New genome editing techniques are opening new avenues to genetic modification development and uses, putting pressure on these frameworks. Here we discuss the implications of definitions of living/genetically modified organisms, the evolving genome editing tools to obtain them and how the regulatory frameworks around the world have taken these technologies into account, with a focus on agricultural crops. Finally, we expand this review beyond commercial crops to address living modified organism uses in food industry, biomedical applications and climate change-oriented solutions.