Browsing by Author "Retamal, Claudio"

Now showing 1 - 5 of 5
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    Anti-Ribosomal P Protein Autoantibodies From Patients With Neuropsychiatric Lupus Impair Memory in Mice
    (2015) Bravo Zehnder, Marcela; Toledo, Enrique M.; Segovia Miranda, Fabian; Serrano, Felipe G.; Benito, Maria J.; Metz Baer, Claudia Andrea; Retamal, Claudio; Álvarez Rojas, Alejandra; Massardo Vega, Loreto; Inestrosa Cantín, Nibaldo
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    Epidermal growth factor receptor endocytic traffic perturbation by phosphatidate phosphohydrolase inhibition : new strategy against cancer
    (2014) Shaughnessy, Ronan Patrick; Retamal, Claudio; Oyanadel, Claudia; Norambuena Pérez, Andrés; López, Alejandro; Bravo Zehnder, Marcela; Montecino, Fabián, J.; Metz Baer, Claudia Andrea; Soza Gajardo, Andrea; González de la Rosa, Alfonso
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    Fact or Fiction, It Is Time for a Verdict on Vasculogenic Mimicry?
    (2019) Valdivia Román, Andrés Felipe; Mingo Orsini, Gabriel Antonio; Aldana, Varina; Pinto, Mauricio P.; Ramírez, Marco; Retamal, Claudio; González, Alfonso; Nualart, Francisco; Corválan, Alejandro H.; Owen, Gareth Ivor
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    Pathogenicity of lupus anti-ribosomal P antibodies : Role of cross-reacting neuronal surface P antigen in glutamatergic transmission and plasticity in a mouse model
    (2015) Segovia Miranda, Fabián; Serrano, Felipe; Dyrda, Agnieszka; Ampuero, Estibaliz; Retamal, Claudio; Bravo Zehnder, Marcela; Parodi, Jorge; Zamorano, Pedro; Valenzuela, David; Massardo Vega, Loreto; Van Zundert, Brigitte; Inestrosa Cantín, Nibaldo; González Alfonso
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    Phosphatidic Acid Induces Ligand-independent Epidermal Growth Factor Receptor Endocytic Traffic through PDE4 Activation
    (AMER SOC CELL BIOLOGY, 2010) Norambuena, Andres; Metz, Claudia; Jung, Juan E.; Silva, Antonia; Otero, Carolina; Cancino, Jorge; Retamal, Claudio; Valenzuela, Juan C.; Soza, Andrea; Gonzalez, Alfonso
    Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to test phosphatidic acid (PA) generated by phospholipase D (PLD) as an endogenous down-regulator of PKA activity, which activates rolipram-sensitive type 4 phosphodiesterases (PDE4) that degrade cAMP. We found that inhibition of PA hydrolysis by propranolol, in the absence of ligand, provokes internalization of inactive (neither tyrosine-phosphorylated nor ubiquitinated) EGFR, accompanied by a transient increase in PA levels and PDE4s activity. This EGFR internalization is mimicked by PA micelles and is strongly counteracted by PLD2 silencing, rolipram or forskolin treatment, and PKA overexpression. Accelerated EGFR endocytosis seems to be mediated by clathrin-dependent and -independent pathways, leading to receptor accumulation in juxtanuclear recycling endosomes, also due to a decreased recycling. Internalized EGFR can remain intracellular without degradation for several hours or return rapidly to the cell surface upon discontinuation of the stimulus. This novel regulatory mechanism of EGFR, also novel function of signaling PA, can transmodulate receptor accessibility in response to heterologous stimuli.