Browsing by Author "Quiroz, Karla."

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    Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
    (2018) Carrasco Gálvez, Basilio Alejandro; Garcia Gonzales, Rolando.; Pico Mendoza, José.; Quiroz, Karla.; Cáceres, Pablo.; Chong Perez, Borys.; Pino, Hugo.; Seiltgens, Marjorie.; Greck, Eglis.; Caligari, Peter D. S.
    Abstract Background Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.