Browsing by Author "Perez-Cotapos, Maria Luisa"
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- ItemComparative analysis of loss of heterozygosity and microsatellite instability in adult and pediatric melanoma.(2005) Uribe González, Pablo Francisco; Wistuba, Ignacio I.; Solar, Antonieta; Balestrini, Claudia; Perez-Cotapos, Maria Luisa; Gonzalez, SergioAlthough 0.3% of melanomas occur in children, the incidence has risen in past decades. In adult melanoma, some chromosomal regions in 1p, 6q, 9p, 10q, and 11q are frequently deleted. Microsatellite instability (MSI), which reflects impaired DNA repair, has been found at low levels in adult melanoma and melanocytic nevi. To investigate the molecular changes in pediatric melanoma, a screening for loss of heterozygosity and microsatellite instability was performed and compared with changes found in adult melanoma. Formalin-fixed, paraffin-embedded tissues from 10 adult melanomas, 9 melanocytic nevi, and 8 pediatric melanomas were microdissected and the DNA was extracted. Loss of heterozygosity and microsatellite instability were evaluated using 13 microsatellite repeat polymorphisms located in 1p36, 1q32, 2p12, 2p22-25, 2q33-37, 9p21, 10q23.3, 11q23, 13q14, 17p13, and 17q21. The overall frequency of loss of heterozygosity was 0.09 for nevi, 0.30 for adult melanoma, and 0.43 for pediatric melanoma (nevi vs. adult melanoma, P = 0.0082; nevi vs. pediatric melanoma, P = 0.0092). Pediatric melanoma has more loss of heterozygosity (44%) in 11q23 than adult melanoma (7%, P = 0.046). The microsatellite instability overall frequency was greater in pediatric melanoma (0.24) than nevi (0.05, P = 0.0031) and adult melanoma (0.09, P = 0.0195). Our findings suggest that pediatric melanoma has a different abnormal pattern than adult melanoma. Pediatric melanoma has more microsatellite instability than adult melanoma. 11q23 could contain genes related to the early age onset of melanoma. The high frequency of microsatellite instability is coincidental with the finding of higher levels of microsatellite instability in pediatric brain tumors and could play a role in the pathogenesis of pediatric melanoma.
- ItemOral and written counseling is a useful instrument to improve short-term adherence to treatment in acne patients: a randomized controlled trial(2015) Navarrete Dechent, Cristián Patricio; Curi Tuma, Maximiliano; Nicklas, Claudia; Cárdenas de la Torre, Consuelo Paz; Perez-Cotapos, Maria Luisa; Salomone, ClaudiaBackground: Therapeutic success in acne patients not only depends in the appropriate selection of drugs but also on patient’s treatment adherence or compliance. Lack of adherence is a very important problem both in general medicine and in dermatologic practice. Objective: To evaluate the impact of oral and written counseling in adherence to treatment in acne patients. Patients and Methods: Eighty patients were randomized into a two study groups of 40 patients each in which instructions were given in a written note (besides oral counseling) and reinforced by a telephone within 15 days of onset, and a control group that received indications as usual (oral counseling in-office only). Both groups were followed-up, evaluating adherence to treatment according to self-reporting of patients at 30, 60, 90 days and 6 months. Results: Better adherence to treatment was observed in the intervention group, being statistically significant only in the first month of treatment (80% versus 62%, p = 0.043). The beneficial effect of written counseling plus a phone call diluted in subsequent months. Conclusion: Written counseling significantly improves adherence in the first month of treatment. Good adherence could also lead to more effective treatments so it seems reasonable to use time and resources to optimize adherence to treatment.