Browsing by Author "Parra Cares, Julio Alejandro"

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    Color Doppler Ultrasound Assessment of Subclinical Activity With Scoring of Morphea
    (2023) Parra Cares, Julio Alejandro; Wortsman Canovas, Ximena Loreto; Alfaro Sepúlveda, Daniela Alejandra; Mellado Francisco, Gonzalo; Ramírez Cornejo, Cristian Alejandro; Vera Kellet, Cristián Andrés
    Background: Detection of activity in morphea is paramount for adequately managing the disease. Subclinical ultrasoundinvolvement on inactive lesions or healthy skin areas adjacent to morphea has not been described to date.Objectives: The study aimed to detect morphea’s subclinical activity by Color Doppler ultrasound not identified with theclinical scorings.Materials & methods: This cross-sectional retrospective study was done from January 2014 to July 2019 in patients witha clinicopathological diagnosis of morphea. The modified Localized Scleroderma Skin Severity Index (mLoSSI) and TheUltrasound Morphea Activity Score (US-MAS) were used to correlate clinical and subclinical activity.Results: A total of 36 patients met the inclusion criteria. 54% of cases presented subclinical activity in areas adjacent to theclinically active lesion, 23% in nonadjacent regions, and 23% demonstrated activity at a clinically inactive lesion site.100% of patients with morphea “en coup de sabre” involving the frontal region of the face concomitantly presented bothsubclinical activities of morphea on the frontal facial region and the scalp following the same axis.A positive relationship was observed between the degree of clinical activity measured by mLoSSI and US-MAS scoring.The main limitations of our study were the low number of patients and the inability to detect alterations < 0.1 mm.Conclusions: Subclinical activity is frequent in morphea, can extend beyond the lesional areas, including apparently noninvolved adjacent and distant corporal regions, and can be detected by color Doppler ultrasound.
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    Npc1 deficiency in the C57BL/6J genetic background enhances Niemann–Pick disease type C spleen pathology
    (2011) Parra Cares, Julio Alejandro; Klein, Andres D.; Castro, Juan Francisco; Morales France, María Gabriela; Mosqueira Montero, Matías José; Valencia Araya, Ilse; Cortés Mora, Víctor Antonio; Rigotti Rivera, Attilio; Zanlungo Matsuhiro, Silvana
    Niemann–Pick type C (NPC) disease is an autosomal recessive neurovisceral lipid storage disorder. The affected genes are NPC1 and NPC2. Mutations in either gene lead to intracellular cholesterol accumulation. There are three forms of the disease, which are categorized based on the onset and severity of the disease: the infantile form, in which the liver and spleen are severely affected, the juvenile form, in which the liver and brain are affected, and the adult form, which affects the brain. In mice, a spontaneous mutation in the Npc1 gene originated in the BALB/c inbred strain mimics the juvenile form of the disease. To study the influence of genetic background on the expression of NPC disease in mice, we transferred the Npc1 mutation from the BALB/c to C57BL/6J inbred background. We found that C57BL/6J-Npc1−/− mice present with a much more aggressive form of the disease, including a shorter lifespan than BALB/c-Npc1−/− mice. Surprisingly, there was no difference in the amount of cholesterol in the brains of Npc1−/− mice of either mouse strain. However, Npc1−/− mice with the C57BL/6J genetic background showed striking spleen damage with a marked buildup of cholesterol and phospholipids at an early age, which correlated with large foamy cell clusters. In addition, C57BL/6J Npc1−/− mice presented red cell abnormalities and abundant ghost erythrocytes that correlated with a lower hemoglobin concentration. We also found abnormalities in white cells, such as cytoplasmic granulation and neutrophil hypersegmentation that included lymphopenia and atypias. In conclusion, Npc1 deficiency in the C57BL6/J background is associated with spleen, erythrocyte, and immune system abnormalities that lead to a reduced lifespan.
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    Performance of a deep neural network in teledermatology : a single-centre prospective diagnostic study
    (2021) Ramirez Cornejo, Cristian Alejandro; Del Barrio Diaz, Pablo Ignacio; Jaque Silva, Ana Alejandra; Uribe González, Pablo Francisco; Majerson Bursztyn, David; Curi Tuma, Maximiliano; Del Puerto Troncoso, Constanza; Reyes Baraona, Francisco Javier; Parra Cares, Julio Alejandro; Vera Kellet, Cristián Andrés; Navarrete Dechent, Cristián Patricio; Muñoz-López, C.; Marchetti, M. A.; Han, S. S.; Meza-Romero, R.; Araneda-Ortega, P.; Guzmán, M.; Millán-Apablaza, R.; Nuñez-Mora, M.; Liopyris, K. ;