Browsing by Author "Pardo, Fabián"
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- ItemAdenosine receptors: Modulators of lipid availability that are controlled by lipid levels(2017) Leiva Mendoza, Andrea Alejandra; Guzmán Gutiérrez, Enrique; Contreras Duarte, Susana de las Mercedes; Fuenzalida Saavedra, Bárbara; Cantin, Claudette; Carvajal, Lorena; Salsoso Rodríguez, M. Rocío; Gutiérrez, Jaime; Pardo, Fabián; Sobrevía Luarte, Luis Alberto
- ItemEscherichia coli Heat-Stable Enterotoxin Mediates Na+/H+ Exchanger 4 Inhibition Involving cAMP in T-84 Human Intestinal Epithelial Cells(2015) Beltrán, Ana R.; Carraro Lacroix, Luciene R.; Bezerra, Camila N. A.; Cornejo, Marcelo; Norambuena, Katrina; Toledo, Fernando; Araos, Joaquín; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sanhueza, Carlos; Malnic, Gerhard; Sobrevía Luarte, Luis Alberto; Ramirez, Marco A.
- ItemInsulin requires normal expression and signaling of insulin receptor A to reverse gestational diabetes-reduced adenosine transport in human umbilical vein endothelium(2015) Westermeier, Francisco; Salomón, Carlos; Farías Jofré, Marcelo Enrique; Arroyo, Pablo; Fuenzalida Saavedra, Bárbara; Sáez, Tamara; Salsoso Rodríguez, M. Rocío; Sanhueza, Carlos; Guzmán-Gutierrez, Enrique; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto
- ItemInsulin reverses endothelial dysfunction via A2B adenosine receptor activation in human umbilical vein endothelium from late-onset preeclampsia(2016) Salsoso Rodríguez, M. Rocío; Leiva Mendoza, Andrea Alejandra; Pardo, Fabián; Chiarello, Indira; Farías Jofré, Marcelo Enrique; Vázquez, Carmen; Sobrevía Luarte, Luis AlbertoPreeclampsia courses with endothelial and insulin resistance. Insulin dilates umbilical vein requiring A2A adenosine receptor (A2AAR) activation in normal pregnancies; however, whether A2AAR are involved in late-onset preeclampsia (LOPE)-reduced insulin dilation is unknown. LOPE increases maternal and foetal plasma adenosine and A2BAR expression in human umbilical vein endothelial cells (HUVECs). A2BAR involvement and insulin effect on the foe- toplacental vascular function in LOPE is unknown. Aim: To evaluate A2AAR and A2BAR involvement on insulin effect on endothelial function in umbilical veins and HUVECs from LOPE. Methods: Protein abundance (total and phosphorylated) of p44/42mapk, protein kinase B/Akt (Akt), endothelial nitric oxide synthase (eNOS) were detected by Western blot. Assays were in the absence or presence (8 h) of 1 nM insulin, 30 nM CGS21680 (A2AAR agonist), 10 nM ZM241385 (A2AAR antagonist), 0.1 nM BAY606583 (A2BAR agonist), or 30 nM MRS1754 (A2BAR antago- nist). Vascular response to insulin (0.1–1000 nM, 5 min) was mea- sured in preconstricted umbilical vein rings (wire myography) in the absence or presence of adenosine (1 mM) and/or A2AAR and A2BAR antagonists. L-Citrulline level was measured by HPLC in the absence or presence (8 h) of NG-nitro- L-arginine methyl ester (100 lM) in HUVECs. Results: Insulin increased Akt (1.3 to 0.1 fold), p44/42mapk (1.2 to 0.1 fold), Ser1177 eNOS phosphorylation (1.2 to 0.01 fold), and total eNOS protein abundance (1.5 to 0.1 fold) in HUVECs from normal pregnancies. A2AAR and A2BAR activation enhanced insulin effect only on Akt (1.6 to 0.1 fold). LOPE only increased Ser1177 eNOS phos- phorylation and total eNOS protein abundance (2.2 to 0.9 and 1.4 to 0.2 fold, respectively). Insulin blocked LOPE-increased Ser1177 eNOS phosphorylation. A2AAR and A2BAR activation did not change insu- lin effect on Akt and p44/42mapk, whereas A2AAR antagonist reversed insulin-decreased Ser1177 eNOS phosphorylation and increased total eNOS protein abundance in LOPE. Insulin dilation of umbilical veins from normal pregnancies was lower (14 to 2%, max- imal relaxation (Rmax)) in the presence of A2AAR. LOPE reduced insulin dilation (18 to 3% Rmax), which was restored by A2AAR antagonists. Insulin increased L-citrulline content (5.3 to 0.3 fold), a phenomenon blocked by A2AAR and A2BAR antagonists in normal pregnancies. LOPE increased L-citrulline content, which was unal- tered by insulin in absence of A2AAR and A2BAR antagonists. However, insulin increased L-citrulline content in the presence of A2AAR antagonists, but blocked by A2BAR antagonists in LOPE. Conclusion: The reduced foetoplacental vascular response to insu- lin in LOPE involves A2AAR activation, a phenomenon counteracted by A2BAR activation.
- ItemPreeclampsia associates with RECK-dependent decrease in human trophoblasts migration and invasion(2017) Gutiérrez, Jaime; Aedo, Alejandro; Mora, Jacob; Maldonado, Jorge; Salsoso Rodríguez, M. Rocío; Toledo, Fernando; Farías Jofré, Marcelo Enrique; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto
- ItemReduced L-Carnitine Transport in Aortic Endothelial Cells from Spontaneously Hypertensive Rats(2014) Salsoso Rodríguez, M. Rocío; Guzmán Gutiérrez, Enrique; Arroyo, Pablo; Salomón, Carlos; Zambrano, Sonia; Ruiz-Armenta, María Victoria; Blanca, Antonio Jesús; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Mate, Alfonso; Sobrevía Luarte, Luis Alberto; Vázquez, Carmen María
- ItemRole for Tetrahydrobiopterin in the Fetoplacental Endothelial Dysfunction in Maternal Supraphysiological Hypercholesterolemia(2016) Leiva Mendoza, Andrea Alejandra; Fuenzalida Saavedra, Bárbara; Westermeier, Francisco; Toledo, Fernando; Salomón, Carlos; Gutiérrez, Jaime; Sanhueza, Carlos; Pardo, Fabián; Sobrevía Luarte, Luis Alberto
- ItemRole of Insulin and Adenosine in the Human Placenta Microvascular and Macrovascular Endothelial Cell Dysfunction in Gestational Diabetes Mellitus(2014) Guzmán Gutiérrez, Enrique; Arroyo, Pablo; Salsoso Rodríguez, M. Rocío; Fuenzalida Saavedra, Bárbara; Sáez, Tamara; Leiva Mendoza, Andrea Alejandra; Pardo, Fabián; Sobrevía Luarte, Luis Alberto
- ItemSodium/proton exchanger isoform 1 regulates intracellular pH and cell proliferation in human ovarian cancer(2017) Sanhueza, Carlos; Araos, Joaquín; Naranjo, Luciano; Toledo, Fernando; Beltrán, Ana R.; Ramirez, Marco A.; Gutiérrez, Jaime; Pardo, Fabián; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto
- ItemTetrahydrobiopterin Role in human umbilical vein endothelial dysfunction in maternal supraphysiological hypercholesterolemia(2016) Leiva Mendoza, Andrea Alejandra; Fuenzalida Saavedra, Bárbara; Salsoso Rodríguez, M. Rocío; Barros, Eric; Toledo, Fernando; Gutiérrez, Jaime; Pardo, Fabián; Sobrevía Luarte, Luis Alberto