Browsing by Author "ORTIZ, ME"

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    DIFFERENTIAL TRANSPORT OF FERTILIZED AND UNFERTILIZED OVA IN PSEUDOPREGNANT RATS
    (SOC BIOL CHILE, 1979) VILLALON, M; ORTIZ, ME; AGUAYO, C; MUNOZ, J; CROXATTO, HB
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    DIFFERENTIAL TRANSPORT OF FERTILIZED AND UNFERTILIZED OVA IN THE RAT
    (SOC STUDY REPRODUCTION, 1982) VILLALON, M; ORTIZ, ME; AGUAYO, C; MUNOZ, J; CROXATTO, HB
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    EFFECT OF ESTRADIOL 3-BENZOATE AND ESTRADIOL 3,17BETA ON OVUM TRANSPORT IN RATS
    (ASSN LATINOAMER CIENC FISIOL, 1975) ORTIZ, ME; VILLALON, M; ALONSO, ML; CROXATTO, HB
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    OVUM TRANSPORT AND FERTILITY FOLLOWING POST-OVULATORY TREATMENT WITH ESTRADIOL IN RATS
    (SOC STUDY REPRODUCTION, 1979) ORTIZ, ME; VILLALON, M; CROXATTO, HB
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    UTERINE KALLIKREIN IN THE EARLY PREGNANT RAT
    (OXFORD UNIV PRESS INC, 1993) VALDES, G; CORTHORN, J; SCICLI, AG; GAETE, V; SOTO, J; ORTIZ, ME; FORADORI, A; SAED, GM
    Uterine homogenates of cycling and early pregnant Sprague Dawley rats and purified rat urinary kallikrein showed similar curves of displacement of I-125-kallikrein binding to a polyclonal antibody. Uterine kallikrein concentration measured by RIA was 8.7 +/- 2 SEM ng/g wet weight during the cycle (n = 6 in diestrus and metestrus) and 20.8 +/- 2 SEM (n = 7) ng/g wet weight on Day 7 of pregnancy (P7) (p < 0.001). On P7, kallikrein concentration was increased 12.4-fold in the implantation nodes, as compared to the interimplantation segments. Uterine homogenates of rats on P7, submitted to DEAE-cellulose chromatography and Sephadex gel filtration, yielded two fractions containing kallikrein immunoreactivity and kininogenase activity, with molecular masses that ranged from 120-125 kDa and 39-43 kDa, respectively. In the RIA, both fractions displayed parallelism with purified kallikrein. Enzymatic activity was expressed after activation by trypsin. It was inhibited by aprotinin, PMSF, p-amino-benzamidine, and leupeptin, but not by soybean or ovomucoid trypsin inhibitors. Kallikrein mRNA was demonstrated by reverse transcriptase/polymerase chain reaction in uteri of nonpregnant and P7 rats. These results show that rat uterus synthesizes one or more serine proteases that are immunologically and enzymatically related to tissue kallikrein. The increase of kallikrein in the implantation node on P7-determined both by an increment of whole uterus kallikrein content and a depletion of the interimplantation segments-suggests that kallikrein may play a role in the vasoactive changes of implantation.