Browsing by Author "Larrondo Castro, Luis Fernando"

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    A circadian clock in Neurospora crassa functions during plant cell wall deconstruction
    (2020) Diaz, R. D.; Larrondo Castro, Luis Fernando
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    Amiodarone versus other pharmacological interventions for prevention of sudden cardiac death
    (2015) Claro, J. C.; Candia Balboa, Roberto; Rada G., Gabriel; Baraona Reyes, Fernando Exequiel; Larrondo Castro, Luis Fernando; Letelier Saavedra, Luz María; Claro, J. C.; Candia Balboa, Roberto; Rada G., Gabriel; Baraona, F.; Larrondo Castro, Luis Fernando; Letelier Saavedra, Luz María
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    An Out-of-Patagonia migration explains the worldwide diversity and distribution of Saccharomyces eubayanus lineages
    (2020) Nespolo Rossi, Roberto; Villarroel, C. A.; Oporto, C. I.; Tapia, S. M.; Vega Macaya, F.; Urbina, K.; De Chiara, M.; Mozzachiodi, S.; Mikhalev, E.; Larrondo Castro, Luis Fernando; Thompson, D.; Saenz Agudelo, P.; Liti, G.; Cubillos, F. A.
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    Assessing the Effects of Light on Differentiation and Virulence of the Plant Pathogen Botrytis cinerea: Characterization of the White Collar Complex
    (2013) Canessa Aguila, Paulo Francisco.; Hevia Hoffmann, Montserrat Alejandra.; Larrondo Castro, Luis Fernando
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    Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa
    (2019) Horta, María Augusta C.; Thieme, Nils; Gao, Yuqian Q.; Burnum Johnson, Kristin E.; Nicora, Carrie D.; Gritsenko, Marina A.; Lipton, Mary S.; Mohanraj, Karthikeyan; De Assis, Leandro José; Larrondo Castro, Luis Fernando; Lin, Liangcai C.; Tian, Chaoguang G.; Braus, Gerhard H.; Borkovich, Katherine A.; Schmoll, Monika; Samal, Areejit.; Goldman, Gustavo H.; Benz, J. Philipp
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    BZIP transcription factors and transcriptional regulatory networks in the neurospora circadian system
    (2014) Montenegro Montero, Alejandro Esteban; Larrondo Castro, Luis Fernando; Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas
    Circadian clocks are endogenous cellular timekeepers that confer daily rhythms to a large number of biological processes. These clocks are present in various organisms across different evolutionary lineages, in which they regulate close to 24-hours rhythms in gene expression, physiology and behavior, enabling individuals to anticipate predictable environmental variations. The ascomycete Neurospora crassa has played a key role in the unveiling of the molecular and genetic basis of these time-telling machineries. In Neurospora, as in other eukaryotes, the integration of a series of cellular and molecular processes gives rise to a robust cell-based pacemaker, capable of coordinating rhythmic control of several aspects of their biology. Although a detailed molecular description of the core oscillator or pacemaker is now possible in model eukaryotes, there is limited information on the mechanisms that allow it to regulate rhythmic processes. Such “output pathways”, the circuits through which the pacemaker endows different processes with rhythmicity, are the least characterized aspect of circadian systems. In Neurospora, a hierarchical arrangement of transcriptional regulators has been proposed as the main mechanism through which the clock regulates rhythmic gene expression.The different actors involved in such time relay, connecting the oscillator with overt rhythms, are however largely unknown. In addition, despite decades as a research model organism, little is known about transcriptional regulatory networks in this fungus and the vast majority of transcription factors in Neurospora remain uncharacterized. In an effort to improve current knowledge of output pathways, the most neglected aspect of circadian biology, in a clock model system and study transcriptional regulatory networks in a model eukaryote, we set out to characterize the bZIP family of transcriptional regulators in Neurospora, in the context of its circadian system. We report a complete revision of the list of sequence-specific DNA-binding proteins in this fungus, which resulted in the identification of several novel ones, including many bZIP proteins. As the few transcription factors that have been associated with output pathways in Neurospora have been shown to exhibit clock input, we evaluated whether the expression of bZIP encoding genes in this organism is under control of the circadian clock. By using a luciferase-based, high-throughput screening system, we identified several bZIP encoding genes whose expression is regulated by the circadian pacemaker. A major limitation in the study of transcriptional regulatory networks in Neurospora, such as those underlying clock regulated transcription, stems from the fact that little is known about the sequence preference of its transcription factors. With the goal of identifying and characterizing transcriptional regulatory networks in which the putative Neurospora transcription factors participate, we employed double-stranded DNA microarrays known as protein-binding microarrays, to determine the sequence preference of Neurospora transcription factors.Such an approach allows for rapid, high-throughput and unbiased characterization of the sequence specificity of DNA-binding proteins. This resulted in the determination of the sequence preference of over half of Neurospora predicted transcription factors, information that together with the various molecular tools available in Neurospora, led to the identification of a rhythmically expressed bZIP transcription factor, ADA-1, as a regulator of output pathways in Neurospora, controlling various output genes. In addition, this information allowed for the evaluation of the role of another bZIP transcription factor, ASL-1, in such pathways. Notably, this is the first report aimed at studying DNA-binding specificities on a global scale in the fungal kingdom outside of the yeast clade, representing a powerful resource for the study of transcriptional regulatory networks in filamentous fungi, the largest group within the fungal kingdom. Indeed, through the use of these data we identified, for the first time, a transcription factor that is required for growth under osmotic stress in Neurospora. Lastly, we report on the identification of a novel process involved in output pathways in Neurospora, namely cell fusion pathways, and we herein show it to be necessary for proper rhythms in a number of genes, including bZIP encoding genes. As a whole, the work reported in this Thesis, represents a major advancement in the study of bZIP proteins and transcriptional regulatory networks in Neurospora.
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    Circadian clocks and the regulation of virulence in fungi : getting up to speed
    (2016) Hevia, M.; Canessa, P.; Larrondo Castro, Luis Fernando
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    Circadian rhythms synchronize mitosis in Neurospora crassa
    (2014) Hong, C.; Larrondo Castro, Luis Fernando; Goity Falconi, Alejandra Isabel.
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    Coupling cell communication and optogenetics: implementation of a light-inducible intercellular system in yeast
    (2023) Rojas Jorquera, Vicente Alberto; Larrondo Castro, Luis Fernando; Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas
    La comunicación celular es un fenómeno extendido en biología, permitiendo la transmisión de información acerca de las condiciones del entorno. Con el fin de entender cómo la comunicación celular modula procesos biológicos relevantes, diferentes sistemas sintéticos basados en inducción química han sido exitosamente desarrollados. En este trabajo, se acopló la comunicación celular y la optogenética en la levadura Saccharomyces cerevisiae. La aproximación consiste en dos poblaciones conectadas por la producción dependiente de luz de la feromona factor-α en un tipo celular, que induce la expresión génica en el otro grupo de células. Después de la caracterización individual de tres variantes de ambas cepas, el sistema intercelular optogenético fue evaluado combinando las diferentes células bajo condiciones contrastantes de iluminación. Usando luciferasa como gen reportero, co-cultivos específicos a una proporción 1:1 muestran activación de la respuesta bajo luz azul constante, lo que no fue observado para las mismas mezclas crecidas en oscuridad. Entonces, el sistema fue evaluado en varias transiciones oscuridad/luz azul, donde el nivel de respuesta varia dependiendo del momento en el que la iluminación fue entregada. Además, la amplitud de la respuesta puede ser ajustada modificando la proporción inicial entre ambos tipos de cepa. También, células expresando todas las partes del circuito sintético se generaron para verificar si la separación de actividades biológicas mejora el rendimiento general del sistema. En ese contexto, el sistema de dos poblaciones mostró mayores veces de inducción en comparación con cepas autónomas. Esta exitosa implementación del sistema intercelular optogenético abrió la oportunidad de expandir el repertorio de las cepas de levadura involucradas variando componentes de la vía de señalización como reguladores negativos y promotores de respuesta a feromona. Así, se obtuvieron diversas versiones del sistema, que muestran un amplio rango de niveles de respuesta bajo regímenes de inducción por luz. En general, estos resultados demuestran que la información externa de la luz es propagada a través de una molécula de señalización difusible para modular la expresión génica en un sistema sintético de células microbianas, lo que pavimentará el camino para estudios que permitan el control optogenético de dinámicas a nivel de población.
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    Daily magnesium fluxes regulate cellular timekeeping and energy balance
    (2016) Kevin, A.; Feeney, Louise L.; Hansen, Marrit Putker; Olivares Yañez, Consuelo; Day, Jason; Eades, Lorna J.; Larrondo Castro, Luis Fernando; Nathaniel, P.; Hoyle, John S; O'Neill; Gerben Van Ooijen
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    Decoupling circadian clock protein turnover from circadian period determination
    (2015) Larrondo Castro, Luis Fernando; Olivares Yáñez, Consuelo del Pilar.
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    Determination and Inference of Eukaryotic Transcription Factor Sequence Specificity
    (2014) Weirauch, M.; Montenegro Montero, Alejandro Esteban.; Goity Falconi, Alejandra Isabel.; Larrondo Castro, Luis Fernando
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    Draft genome sequence of a monokaryotic model brown-rot fungus Postia (Rhodonia) placenta SB12
    (2017) Gaskell, J,; Kersten, P; Larrondo Castro, Luis Fernando; Canessa, P; Martínez, D; Hibbett, D; Schmoll, M|Kubicek, C P; Martínez A T; Yadav, J|Master, E.; Magnuson, J K; Yaver, D; Berka, R; Lail, K; Chen, C; Labutti, K; Nolan, M; Lipzen, A; Aerts, A; Barry, K; Henrissat, B Blanchet, Cullen, D.