Browsing by Author "Larrondo Castro, Luis Fernando"
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- ItemA circadian clock in Neurospora crassa functions during plant cell wall deconstruction(2020) Diaz, R. D.; Larrondo Castro, Luis Fernando
- ItemA developmental cycle masks output from the circadian oscillator under conditions of choline deficiency in Neurospora(2007) Shi M; Larrondo Castro, Luis Fernando
- ItemA kinetic study of the effects of light on circadian rhythmicity of the frq promoter of neurospora crassa(2014) Larrondo Castro, Luis Fernando
- ItemA Novel Extracellular Multicopper Oxidase From Phenerochaete Chrysosporium With Ferroxidase Activity(2003) Larrondo Castro, Luis Fernando; Melo Ledermann, Francisco Javier; Vicuña, Rafael
- ItemAmiodarone versus other pharmacological interventions for prevention of sudden cardiac death(2015) Claro, J. C.; Candia Balboa, Roberto; Rada G., Gabriel; Baraona Reyes, Fernando Exequiel; Larrondo Castro, Luis Fernando; Letelier Saavedra, Luz María; Claro, J. C.; Candia Balboa, Roberto; Rada G., Gabriel; Baraona, F.; Larrondo Castro, Luis Fernando; Letelier Saavedra, Luz María
- ItemAn Out-of-Patagonia migration explains the worldwide diversity and distribution of Saccharomyces eubayanus lineages(2020) Nespolo Rossi, Roberto; Villarroel, C. A.; Oporto, C. I.; Tapia, S. M.; Vega Macaya, F.; Urbina, K.; De Chiara, M.; Mozzachiodi, S.; Mikhalev, E.; Larrondo Castro, Luis Fernando; Thompson, D.; Saenz Agudelo, P.; Liti, G.; Cubillos, F. A.
- ItemAnalysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood(2014) Canessa Aguila, Paulo Francisco.; Larrondo Castro, Luis Fernando
- ItemAssessing the Effects of Light on Differentiation and Virulence of the Plant Pathogen Botrytis cinerea: Characterization of the White Collar Complex(2013) Canessa Aguila, Paulo Francisco.; Hevia Hoffmann, Montserrat Alejandra.; Larrondo Castro, Luis Fernando
- Itembcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea(2015) Canessa Aguila, Paulo Francisco.; Larrondo Castro, Luis Fernando
- ItemBright to dim oscillatory response of the neurospora circadian oscillator(2014) Larrondo Castro, Luis Fernando
- ItemBroad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa(2019) Horta, María Augusta C.; Thieme, Nils; Gao, Yuqian Q.; Burnum Johnson, Kristin E.; Nicora, Carrie D.; Gritsenko, Marina A.; Lipton, Mary S.; Mohanraj, Karthikeyan; De Assis, Leandro José; Larrondo Castro, Luis Fernando; Lin, Liangcai C.; Tian, Chaoguang G.; Braus, Gerhard H.; Borkovich, Katherine A.; Schmoll, Monika; Samal, Areejit.; Goldman, Gustavo H.; Benz, J. Philipp
- ItemBZIP transcription factors and transcriptional regulatory networks in the neurospora circadian system(2014) Montenegro Montero, Alejandro Esteban; Larrondo Castro, Luis Fernando; Pontificia Universidad Católica de Chile. Facultad de Ciencias BiológicasCircadian clocks are endogenous cellular timekeepers that confer daily rhythms to a large number of biological processes. These clocks are present in various organisms across different evolutionary lineages, in which they regulate close to 24-hours rhythms in gene expression, physiology and behavior, enabling individuals to anticipate predictable environmental variations. The ascomycete Neurospora crassa has played a key role in the unveiling of the molecular and genetic basis of these time-telling machineries. In Neurospora, as in other eukaryotes, the integration of a series of cellular and molecular processes gives rise to a robust cell-based pacemaker, capable of coordinating rhythmic control of several aspects of their biology. Although a detailed molecular description of the core oscillator or pacemaker is now possible in model eukaryotes, there is limited information on the mechanisms that allow it to regulate rhythmic processes. Such “output pathways”, the circuits through which the pacemaker endows different processes with rhythmicity, are the least characterized aspect of circadian systems. In Neurospora, a hierarchical arrangement of transcriptional regulators has been proposed as the main mechanism through which the clock regulates rhythmic gene expression.The different actors involved in such time relay, connecting the oscillator with overt rhythms, are however largely unknown. In addition, despite decades as a research model organism, little is known about transcriptional regulatory networks in this fungus and the vast majority of transcription factors in Neurospora remain uncharacterized. In an effort to improve current knowledge of output pathways, the most neglected aspect of circadian biology, in a clock model system and study transcriptional regulatory networks in a model eukaryote, we set out to characterize the bZIP family of transcriptional regulators in Neurospora, in the context of its circadian system. We report a complete revision of the list of sequence-specific DNA-binding proteins in this fungus, which resulted in the identification of several novel ones, including many bZIP proteins. As the few transcription factors that have been associated with output pathways in Neurospora have been shown to exhibit clock input, we evaluated whether the expression of bZIP encoding genes in this organism is under control of the circadian clock. By using a luciferase-based, high-throughput screening system, we identified several bZIP encoding genes whose expression is regulated by the circadian pacemaker. A major limitation in the study of transcriptional regulatory networks in Neurospora, such as those underlying clock regulated transcription, stems from the fact that little is known about the sequence preference of its transcription factors. With the goal of identifying and characterizing transcriptional regulatory networks in which the putative Neurospora transcription factors participate, we employed double-stranded DNA microarrays known as protein-binding microarrays, to determine the sequence preference of Neurospora transcription factors.Such an approach allows for rapid, high-throughput and unbiased characterization of the sequence specificity of DNA-binding proteins. This resulted in the determination of the sequence preference of over half of Neurospora predicted transcription factors, information that together with the various molecular tools available in Neurospora, led to the identification of a rhythmically expressed bZIP transcription factor, ADA-1, as a regulator of output pathways in Neurospora, controlling various output genes. In addition, this information allowed for the evaluation of the role of another bZIP transcription factor, ASL-1, in such pathways. Notably, this is the first report aimed at studying DNA-binding specificities on a global scale in the fungal kingdom outside of the yeast clade, representing a powerful resource for the study of transcriptional regulatory networks in filamentous fungi, the largest group within the fungal kingdom. Indeed, through the use of these data we identified, for the first time, a transcription factor that is required for growth under osmotic stress in Neurospora. Lastly, we report on the identification of a novel process involved in output pathways in Neurospora, namely cell fusion pathways, and we herein show it to be necessary for proper rhythms in a number of genes, including bZIP encoding genes. As a whole, the work reported in this Thesis, represents a major advancement in the study of bZIP proteins and transcriptional regulatory networks in Neurospora.
- ItemChapter Four - Around the Fungal Clock: Recent Advances in the Molecular Study of Circadian Clocks in Neurospora and Other Fungi(2015) Montenegro, A.; Canessa, P.; Larrondo Castro, Luis Fernando
- ItemCharacterization of a multicopper oxidase gene cluster in Phanerochaete chrysosporium and evidence of altered splicing of the mco transcripts(2004) Larrondo Castro, Luis Fernando; González Ojeda, Bernardo; Vicuña, Rafael; Larrondo Castro, Luis Fernando; González Ojeda, Bernardo; Vicuña, Rafael
- ItemCharacterization of PIR1, a GATA family transcription factor involved in iron responses in the white-rot fungus Phanerochaete chrysosporium(2012) Canessa Aguila, Paulo Francisco.; Muñoz Guzmán, Felipe Andrés.; Vicuña, Rafael; Larrondo Castro, Luis Fernando; Canessa Aguila, Paulo Francisco.; Muñoz Guzmán, Felipe Andrés.; Vicuña, Rafael; Larrondo Castro, Luis Fernando
- ItemCircadian clocks and the regulation of virulence in fungi : getting up to speed(2016) Hevia, M.; Canessa, P.; Larrondo Castro, Luis Fernando
- ItemCircadian rhythms synchronize mitosis in Neurospora crassa(2014) Hong, C.; Larrondo Castro, Luis Fernando; Goity Falconi, Alejandra Isabel.
- ItemCloning and characterization of the genes encoding the high-affinity iron-uptake protein complex Fet3/Ftr1 in the basidiomycete Phanerochaete chrysosporium(2007) Larrondo Castro, Luis Fernando; Melo Ledermann, Francisco Javier; Vicuña, Rafael; Larrondo Castro, Luis Fernando; Melo Ledermann, Francisco Javier; Vicuña, Rafael
- ItemCloning and Functional Characterization of the Gene Encoding the Transcription Factor Ace1 in the Basidiomycete Phanerochaete Chrysosporium(2006) Polanco, R.; Larrondo Castro, Luis Fernando; Vicuña, Rafael; Polanco, R.; Larrondo Castro, Luis Fernando; Vicuña, Rafael
- ItemComparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis(2012) Fernández, E.; Canessa Aguila, Paulo Francisco.; Larrondo Castro, Luis Fernando; Vicuña, Rafael; Fernández, E.; Canessa Aguila, Paulo Francisco.; Larrondo Castro, Luis Fernando; Vicuña, Rafael
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